| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra005624 |
| Family | GT48 |
| Protein Properties | Length: 1956 Molecular Weight: 225868 Isoelectric Point: 9.5081 |
| Chromosome | Chromosome/Scaffold: 05 Start: 6592268 End: 6602240 |
| Description | glucan synthase-like 3 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
 | |||
| Family | Start | End | Evalue |
| GT48 | 1042 | 1806 | 0 |
| VPSNLEARRRLTFFSNSLFMEMPDAPKIRNMLSFSVLTPYYQEDVLFSIFGLEKQNEDGVSILFYLQKIFPDEWTNFLERIKCGSEEEIRAREDLEEELR LWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKDEELMKGYKALELTSEDASKSGKSLWAQCQALADMKFTFVVSCQQYSIQKRSGDQRAKDILRLMTT YPSLRVAYIDEVEQTHKDSYKGADDKIYYSALVKAAPQTKPMDSSESVQTLDQVIYRIKLPGPAILGEGKPENQNHAIIFTRGEGLQTIDMNQDNYMEEA FKMRILLQEFLEKHGGVRTPTILGLREHIFTGSVSSLAWFMSNQENSFVTIGQRVLASPLKVRFHYGHPDVFDRLFHLTRGGVCKASKVINLSEDIFAGT CFNSLLLVTRFNSTLREGNVTHHEYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIGFYFSTMLTVLTVYVFLYGRL YLVLSGLEEGLSNQKAFRSNMPLQAALASQSFVQIGFLMALPMMMEIGLERGFHNALIDFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRG FVVFHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGHAYRGVVTYILITVSIWFMVVTWLFAPFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPPEK SWESWWEKEIGHLRHSGKRGIILEIVLALRFFIFQYGLVYQLSTFKQENQSLWIYGASWFVILFI | |||
| Full Sequence |
|---|
| Protein Sequence Length: 1956 Download |
| MSQRRGGGGG PDPPPPQRRI LRTQTAGNLG EAMLDSEVVP SSLVEIAPIL RVANEVEASN 60 PRVAYLCRFY AFEKAHRLDP TSSGRGVRQF KTALLQRLER ENETTLAGRQ KSDAREMQSF 120 YQHYYKKYIQ ALLNAADKAD RAQLTKAYQT AAVLFEVLKA VNQTEDVEVA DEILEAHTKV 180 EEKSQIYVPY NILPLDPDSQ NQAIMRFPEI QATVIALRNT RGLPWPAGHK KKLDEDMLDW 240 LQTMFGFQKD NVANQREHLI LLLANVHIRQ FPRPEQQPKL DDRALTIVMK KLFKNYKKWC 300 KYLGRKSSLW LPTIQQEVQQ RKLLYMGLYL LIWGEAANLR FMPECLCYIY HHMAFELYGM 360 LAGSVSPTTG EHIKPAYGGD DEAFLQKVVT PIYKTIAKEA KRSRGGKSKH SEWRNYDDLN 420 EYFWSVRCFR LGWPMRADAD FFCQTAEELR LDKGEDKPKT GDRWMGKVNF VEIRSFWHIF 480 RSFDRMWSFY ILSLQAMIII AWNGSGDLGA IFHGDVFLKV LSIFITAAIL KLAQAVLDIA 540 LSWKSRHSMS FHAKLRFILK AIAAAVWVVL MPVTYAYSWR SPSGIAETIK NWLGGHSGSS 600 PSLFIMVILI YLSPNMLSTL LFVFPFIRRY LERSDIKIVM LMMWWSQPRL YIGRGMHESA 660 WSLFKYTMYW VVLLISKLAF SFYAEIKPLV VPTKDIMRVH ISVYRWHEFF PHAKNNLGVV 720 VALWSPVILV YFMDTQIWYA IVSTLVGGLN GAFRRLGEIR TLGMLRSRFQ SLPGAFNACL 780 VPNEKSETAK KRGIRATFSR KFDQIPSSKD KEAARFAQMW NKIISSFREE DLISNREMEL 840 LLVPYWADPD LDLIRWPPFL LASKIPIALD MAKDSNGKDR ELKKRLSIDS YMTCAVRECY 900 ASFRNLINFL VVGERERQVI NEIFAKIDEY IANETLIETL DLSALPDLYG QFVRLIEYLM 960 ENKEEDKDQI VIVLLNMLEV VTRDIMDYEV PSLLETAHNG TYVKYDVMTP LHQQKKYFSQ 1020 LRFPVYSQTE AWKEKAKSAM DVPSNLEARR RLTFFSNSLF MEMPDAPKIR NMLSFSVLTP 1080 YYQEDVLFSI FGLEKQNEDG VSILFYLQKI FPDEWTNFLE RIKCGSEEEI RAREDLEEEL 1140 RLWASYRGQT LTKTVRGMMY YRKALELQAF LDMAKDEELM KGYKALELTS EDASKSGKSL 1200 WAQCQALADM KFTFVVSCQQ YSIQKRSGDQ RAKDILRLMT TYPSLRVAYI DEVEQTHKDS 1260 YKGADDKIYY SALVKAAPQT KPMDSSESVQ TLDQVIYRIK LPGPAILGEG KPENQNHAII 1320 FTRGEGLQTI DMNQDNYMEE AFKMRILLQE FLEKHGGVRT PTILGLREHI FTGSVSSLAW 1380 FMSNQENSFV TIGQRVLASP LKVRFHYGHP DVFDRLFHLT RGGVCKASKV INLSEDIFAG 1440 TCFNSLLLVT RFNSTLREGN VTHHEYIQVG KGRDVGLNQI SMFEAKIANG NGEQTLSRDL 1500 YRLGHRFDFF RMLSCYFTTI GFYFSTMLTV LTVYVFLYGR LYLVLSGLEE GLSNQKAFRS 1560 NMPLQAALAS QSFVQIGFLM ALPMMMEIGL ERGFHNALID FVLMQLQLAS VFFTFQLGTK 1620 THYYGRTLFH GGAEYRGTGR GFVVFHAKFA ENYRFYSRSH FVKGIELMIL LLVYQIFGHA 1680 YRGVVTYILI TVSIWFMVVT WLFAPFLFNP SGFEWQKIVD DWTDWNKWIY NRGGIGVPPE 1740 KSWESWWEKE IGHLRHSGKR GIILEIVLAL RFFIFQYGLV YQLSTFKQEN QSLWIYGASW 1800 FVILFILLIV KGLGMGRRRF STNFQLLFRI IKGLVFLTFL AILITFIALR LLTPKDILLC 1860 MLAFMPTGWG MLLIAQACKP LIQRLGIWSS VRTLARGYEI LMGLLLFTPV AFLAWFPFVS 1920 EFQTRMLFNQ AFSRGLQISR ILGGQRKDRS SKNKE* |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
 | ||||||||
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam04652 | DUF605 | 8.0e-13 | 46 | 159 | 122 | + Vta1 like. Vta1 (VPS20-associated protein 1) is a positive regulator of Vps4. Vps4 is an ATPase that is required in the multivesicular body (MVB) sorting pathway to dissociate the endosomal sorting complex required for transport (ESCRT). Vta1 promotes correct assembly of Vps4 and stimulates its ATPase activity through its conserved Vta1/SBP1/LIP5 region. | ||
| pfam14288 | FKS1_dom1 | 2.0e-56 | 320 | 436 | 118 | + 1,3-beta-glucan synthase subunit FKS1, domain-1. The FKS1_dom1 domain is likely to be the 'Class I' region just N-terminal to the first set of transmembrane helices that is involved in 1,3-beta-glucan synthesis itself. This family is found on proteins with family Glucan_synthase, pfam02364. | ||
| pfam02364 | Glucan_synthase | 0 | 1042 | 1807 | 829 | + 1,3-beta-glucan synthase component. This family consists of various 1,3-beta-glucan synthase components including Gls1, Gls2 and Gls3 from yeast. 1,3-beta-glucan synthase EC:2.4.1.34 also known as callose synthase catalyzes the formation of a beta-1,3-glucan polymer that is a major component of the fungal cell wall. The reaction catalyzed is:- UDP-glucose + {(1,3)-beta-D-glucosyl}(N) <=> UDP + {(1,3)-beta-D-glucosyl}(N+1). | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0000148 | 1,3-beta-D-glucan synthase complex |
| GO:0003843 | 1,3-beta-D-glucan synthase activity |
| GO:0006075 | (1->3)-beta-D-glucan biosynthetic process |
| GO:0016020 | membrane |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAD30609.1 | 0 | 1 | 1955 | 1 | 1878 | AC007153_1 Highly similar to putative callose synthase catalytic subunit [Arabidopsis thaliana] |
| GenBank | AAK37413.1 | 0 | 1 | 1955 | 1 | 1950 | AF237733_1 callose synthase 1 catalytic subunit [Arabidopsis thaliana] |
| RefSeq | NP_563743.2 | 0 | 1 | 1955 | 1 | 1950 | CALS1 (CALLOSE SYNTHASE 1); 1,3-beta-glucan synthase/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | NP_850178.1 | 0 | 1 | 1955 | 1 | 1959 | ATGSL03 (GLUCAN SYNTHASE-LIKE 3); 1,3-beta-glucan synthase/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| Swiss-Prot | Q9SL03 | 0 | 1 | 1955 | 1 | 1951 | CALS2_ARATH RecName: Full=Callose synthase 2; AltName: Full=1,3-beta-glucan synthase; AltName: Full=Protein GLUCAN SYNTHASE-LIKE 3 |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2lxm_A | 0.008 | 50 | 169 | 21 | 139 | B Chain B, Lip5-chmp5 |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| callose biosynthesis | 13-BETA-GLUCAN-SYNTHASE-RXN | EC-2.4.1.34 | 1,3-β-glucan synthase |
