| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra006129 |
| Family | GH32 |
| Protein Properties | Length: 801 Molecular Weight: 90367.8 Isoelectric Point: 4.9904 |
| Chromosome | Chromosome/Scaffold: 03 Start: 2133594 End: 2139231 |
| Description | 6-&1-fructan exohydrolase |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 551 | 689 | 4.1e-37 |
| VAGPLYYKGFYHLFYQHNPLNPFFGDIMVWGHSVSQDLVNWIKLEPALSPSVPSDINSFWSGSTTILPDGKPIILYTGNDANKHQVTVLAEPKDPSDPLL REWVKPEGNPVMVPLPPSNVPVHCFRDPTTAWQEHDVSM | |||
| GH32 | 19 | 344 | 0 |
| HFQPQRNWLNAPMYYKGFYHLFYQHNPLAPEFSRRIIWGHSVSQDMVNWIQLPPALSPSESYDINSCWSGSATILPDGKPVILYTGIDNQERREDRRQVT VLAVPKDDSDPLLREWVKPTQNPVMVPPEDILHYCFRDPTTAWQGQDGKWRVLIGAKERDTLRGVALLYHSTDDCEQWTRYQEPLLVAQANEMLECVDFF PVKLMGKEGVDTSVNNASVRHVLKVSFEEELGGKDCYVIGSYCSETDRFVPDSELTYTRADLRYDDGWFYASKSFFDSAKNRRINWGWVVETDSREDDIE KGWAGLLGLPRQMWLDTSGKRLMQWP | |||
| Full Sequence |
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| Protein Sequence Length: 801 Download |
| MAEDGNVQNL PVLFRTSFHF QPQRNWLNAP MYYKGFYHLF YQHNPLAPEF SRRIIWGHSV 60 SQDMVNWIQL PPALSPSESY DINSCWSGSA TILPDGKPVI LYTGIDNQER REDRRQVTVL 120 AVPKDDSDPL LREWVKPTQN PVMVPPEDIL HYCFRDPTTA WQGQDGKWRV LIGAKERDTL 180 RGVALLYHST DDCEQWTRYQ EPLLVAQANE MLECVDFFPV KLMGKEGVDT SVNNASVRHV 240 LKVSFEEELG GKDCYVIGSY CSETDRFVPD SELTYTRADL RYDDGWFYAS KSFFDSAKNR 300 RINWGWVVET DSREDDIEKG WAGLLGLPRQ MWLDTSGKRL MQWPIQEINN LRTRQVSFHN 360 RELEGRSMFE ITGITAAQAD VEVTFDLPVL EDNSRIRDAT HVADAVLFNS GNSLGCVYGP 420 FGLLALASND LSEQTAIFFK IIRRGNGYSV IMGSDENRSS LRENVQKIPH GTFLDIDPRH 480 EKISLRCLID HSIIESYGAG GRNVITSRVY PKLAIGEAAK LYAFNNGTKA TFDGISSLSI 540 ASSSLEMVSG VAGPLYYKGF YHLFYQHNPL NPFFGDIMVW GHSVSQDLVN WIKLEPALSP 600 SVPSDINSFW SGSTTILPDG KPIILYTGND ANKHQVTVLA EPKDPSDPLL REWVKPEGNP 660 VMVPLPPSNV PVHCFRDPTT AWQEHDVSMP LLESQVTGMW ECVDFFPVSV TGKEGFDNSV 720 NDSSVRHVLK TSYGGNDCYV IGTYSSETET FSADSEFNTA ADLRYDYEIY LRPRLSSIVL 780 RIGGSHGDGL WREIAMKMIL * 840 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| smart00640 | Glyco_32 | 4.0e-60 | 553 | 767 | 251 | + Glycosyl hydrolases family 32. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-65 | 553 | 767 | 252 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| cd08996 | GH32_B_Fructosidase | 2.0e-75 | 25 | 347 | 333 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-116 | 19 | 344 | 336 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 6.0e-142 | 19 | 501 | 493 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAB87665.1 | 0 | 6 | 556 | 8 | 547 | fructosidase-like protein [Arabidopsis thaliana] |
| EMBL | CAB87665.1 | 0 | 553 | 770 | 31 | 281 | fructosidase-like protein [Arabidopsis thaliana] |
| RefSeq | NP_001078574.1 | 0 | 6 | 409 | 8 | 407 | AtcwINV6 (6-&1-fructan exohydrolase); hydrolase, hydrolyzing O-glycosyl compounds / inulinase/ levanase [Arabidopsis thaliana] |
| RefSeq | NP_568254.1 | 0 | 6 | 556 | 8 | 550 | AtcwINV6 (6-&1-fructan exohydrolase); hydrolase, hydrolyzing O-glycosyl compounds / inulinase/ levanase [Arabidopsis thaliana] |
| RefSeq | NP_568254.1 | 0 | 553 | 770 | 34 | 284 | AtcwINV6 (6-&1-fructan exohydrolase); hydrolase, hydrolyzing O-glycosyl compounds / inulinase/ levanase [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2aey_A | 0 | 8 | 529 | 1 | 516 | A Chain A, Recombinant Horseradish Peroxidase C Complex With Ferulic Acid |
| PDB | 2aey_A | 0 | 553 | 767 | 25 | 270 | A Chain A, Recombinant Horseradish Peroxidase C Complex With Ferulic Acid |
| PDB | 2ade_A | 0 | 8 | 529 | 1 | 516 | A Chain A, Recombinant Horseradish Peroxidase C Complex With Ferulic Acid |
| PDB | 2ade_A | 0 | 553 | 767 | 25 | 270 | A Chain A, Recombinant Horseradish Peroxidase C Complex With Ferulic Acid |
| PDB | 2add_A | 0 | 8 | 529 | 1 | 516 | A Chain A, Recombinant Horseradish Peroxidase C Complex With Ferulic Acid |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| fructan degradation | RXN-1841 | EC-3.2.1.80 | fructan β-fructosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| FD956604 | 219 | 281 | 499 | 0 |
| CT842376 | 524 | 13 | 527 | 0 |
| CT842376 | 249 | 553 | 767 | 0 |
| FD952457 | 193 | 339 | 531 | 0 |
| FD956604 | 23 | 778 | 800 | 0.55 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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