| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra006247 |
| Family | CE10 |
| Protein Properties | Length: 432 Molecular Weight: 47492.4 Isoelectric Point: 8.0844 |
| Chromosome | Chromosome/Scaffold: 03 Start: 2682729 End: 2684650 |
| Description | carboxyesterase 16 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE10 | 80 | 421 | 0 |
| SALSPTEPRSDRRHSLTPLNHSSPAPETRRNSYGSENLEPYGGYAPSPKRSSRKLPVMLQFHGGGWVSGGSDSAANDFFCRRIAKVCDVIVLAVGYRLAP ENRYPAAFEDGVKVLHWLGKQANLADCCKSLGNNNRRVNGVELKKVNVQGHIVDAFGASMVEPWLAAHADPSRCVLLGVSCGGNIADYVARKAVEAGKLL DPVKVVAQVLMYPFFIGNNPTQSEIKLANSYFYDKPVSVLAWKLFLPEKEFDFDHPAANPLAHNRSGPPLKLMPPTLTVVAEHDWMRDRAIAYAEELRKV NVDSPVLEYKDAVHEFATLDMLLKTPQAQACAEDIAIWVKKY | |||
| Full Sequence |
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| Protein Sequence Length: 432 Download |
| MPSVAVKLYS VFFKLLLKHR LQSLISISPD HAPPDSFGVS TRSDESVAAA NPSFADGVAT 60 KDIHIDPMTS LTVRIFLPES ALSPTEPRSD RRHSLTPLNH SSPAPETRRN SYGSENLEPY 120 GGYAPSPKRS SRKLPVMLQF HGGGWVSGGS DSAANDFFCR RIAKVCDVIV LAVGYRLAPE 180 NRYPAAFEDG VKVLHWLGKQ ANLADCCKSL GNNNRRVNGV ELKKVNVQGH IVDAFGASMV 240 EPWLAAHADP SRCVLLGVSC GGNIADYVAR KAVEAGKLLD PVKVVAQVLM YPFFIGNNPT 300 QSEIKLANSY FYDKPVSVLA WKLFLPEKEF DFDHPAANPL AHNRSGPPLK LMPPTLTVVA 360 EHDWMRDRAI AYAEELRKVN VDSPVLEYKD AVHEFATLDM LLKTPQAQAC AEDIAIWVKK 420 YISLRGHEFS Y* 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00312 | Esterase_lipase | 5.0e-6 | 123 | 196 | 83 | + Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate. | ||
| PRK10162 | PRK10162 | 2.0e-6 | 119 | 202 | 89 | + acetyl esterase; Provisional | ||
| pfam00135 | COesterase | 1.0e-7 | 123 | 179 | 60 | + Carboxylesterase family. | ||
| COG0657 | Aes | 3.0e-35 | 64 | 415 | 359 | + Esterase/lipase [Lipid metabolism] | ||
| pfam07859 | Abhydrolase_3 | 2.0e-61 | 137 | 396 | 260 | + alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAS99713.1 | 0 | 1 | 431 | 1 | 428 | At3g27320 [Arabidopsis thaliana] |
| EMBL | CAB87770.1 | 0 | 1 | 431 | 1 | 439 | putative protein [Arabidopsis thaliana] |
| RefSeq | NP_001030781.1 | 0 | 1 | 431 | 1 | 428 | hydrolase [Arabidopsis thaliana] |
| RefSeq | NP_568298.1 | 0 | 1 | 431 | 1 | 446 | AtCXE16 (Arabidopsis thaliana carboxyesterase 16); hydrolase |
| RefSeq | XP_002526925.1 | 0 | 1 | 431 | 1 | 472 | conserved hypothetical protein [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2zsi_A | 5e-33 | 47 | 398 | 61 | 331 | A Chain A, Characterization And Engineering Of The Bifunctional N- And O-glucosyltransferase Involved In Xenobiotic Metabolism In Plants |
| PDB | 2zsh_A | 5e-33 | 47 | 398 | 61 | 331 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 3ed1_F | 2e-29 | 134 | 398 | 112 | 330 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 3ed1_E | 2e-29 | 134 | 398 | 112 | 330 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 3ed1_D | 2e-29 | 134 | 398 | 112 | 330 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| formononetin biosynthesis | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| formononetin biosynthesis | RXN-3625 | - | 2,7-dihydroxy-4'-methoxyisoflavanone dehydratase |
| isoflavonoid biosynthesis I | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-3303 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-5502 | - | 2,7,5-trihydroxy-4'-methoxyisoflavanone dehydratase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EX130706 | 271 | 162 | 432 | 0 |
| ES789976 | 341 | 106 | 432 | 0 |
| EV055706 | 230 | 161 | 390 | 0 |
| EB438845 | 281 | 152 | 432 | 0 |
| JK515504 | 270 | 163 | 432 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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| Phylogeny |
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