| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra008366 |
| Family | GT20 |
| Protein Properties | Length: 929 Molecular Weight: 104523 Isoelectric Point: 7.3076 |
| Chromosome | Chromosome/Scaffold: 02 Start: 15091980 End: 15101240 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 91 | 558 | 0 |
| NRQRLLVVANRLPVSAVRRGEDSWSLEISAGGLVSALLGVKEFDARWIGWAGVNVPDEVGQKSLTKTLAEKRCIPVFLDEEIVHQYYNGYCNNILWPLFH YLGLPQEDRLATTRSFQSQFAAYKKANLMFADVVNEHYEEGDVVWCHDYHLMFLPKCLKEYNSKMKVGWFLRTPFPSSEIHRTLPSRSELLRSVLAADLV GIYSTYARHFVSACTRILGLEGTPEGVEDQGKLTRVAAFPIGIDSERFIRALEVPDVIQHMKELKERFAGRKVMLGVDRLDIIKGIPQKILAFEKFLEEN ASWRDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTAAPIHHLDRSLDFYALCALYAVTDVALVTSLRDGMNLVSYEFVACQEAKKGVLIL SEFAGAAQSLGAGAILVNPWNITEVAASIGQALNMSSEEREKRHRHNFHHVKTHTSQEWAETFVSELN | |||
| Full Sequence |
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| Protein Sequence Length: 929 Download |
| MPGDKYNSSS YHIPLSRTER LLRERELREK RRSNRARNPN EAVGSSENSD NDLRLEGDSS 60 RQYVEQYLEG AAASARAHDN VCERQEVRPY NRQRLLVVAN RLPVSAVRRG EDSWSLEISA 120 GGLVSALLGV KEFDARWIGW AGVNVPDEVG QKSLTKTLAE KRCIPVFLDE EIVHQYYNGY 180 CNNILWPLFH YLGLPQEDRL ATTRSFQSQF AAYKKANLMF ADVVNEHYEE GDVVWCHDYH 240 LMFLPKCLKE YNSKMKVGWF LRTPFPSSEI HRTLPSRSEL LRSVLAADLV GIYSTYARHF 300 VSACTRILGL EGTPEGVEDQ GKLTRVAAFP IGIDSERFIR ALEVPDVIQH MKELKERFAG 360 RKVMLGVDRL DIIKGIPQKI LAFEKFLEEN ASWRDKVVLL QIAVPTRTDV PEYQKLTSQV 420 HEIVGRINGR FGTLTAAPIH HLDRSLDFYA LCALYAVTDV ALVTSLRDGM NLVSYEFVAC 480 QEAKKGVLIL SEFAGAAQSL GAGAILVNPW NITEVAASIG QALNMSSEER EKRHRHNFHH 540 VKTHTSQEWA ETFVSELNDT VIEAQLRISK VPPELPQNDA IRRYSKSTNR LLILGFNATL 600 TEPVDNQGRR GDQIKEMDLN LHPELKGPLK ALCSDPNTTI VVLSGSSRSV LDKNFGEYDM 660 WLAAENGMFL RLTNGEWMTT MPEHLNMEWV DSVKHVFKYF TERTPRSHFE TRDTSLIWNY 720 KYADIEFGRL QARDLLQHLW TGPISNASVD VVQGSRSVEV RAVGVTKGAA IDRILGEIVH 780 SKSMTTPIDY VLCIGHFLGK DEDVYTFFEP ELPSDIIPAI ARPRPSSDSG TKSSPGDRKP 840 PSKSTHSKKH GSKSSSSSNS YNNNKASQRL SPEKISWNVL EHKGENYFSC AVGRTRTSAR 900 YLLGSPDDVV CFLEKLADTT VPNSSSSS* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 0 | 1 | 923 | 938 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 92 | 928 | 840 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| TIGR02400 | trehalose_OtsA | 0 | 94 | 559 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| pfam00982 | Glyco_transf_20 | 0 | 93 | 559 | 475 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PRK14501 | PRK14501 | 0 | 94 | 795 | 712 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABO61742.1 | 0 | 1 | 926 | 1 | 926 | trehalose-phosphate synthase 1 [Solanum lycopersicum] |
| EMBL | CAA69879.1 | 0 | 1 | 920 | 1 | 939 | trehalose-6-phosphate synthase [Arabidopsis thaliana] |
| RefSeq | NP_177979.1 | 0 | 1 | 920 | 1 | 939 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002331300.1 | 0 | 1 | 920 | 1 | 921 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002531237.1 | 0 | 1 | 917 | 1 | 920 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1gz5_D | 0 | 94 | 561 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_C | 0 | 94 | 561 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_B | 0 | 94 | 561 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_A | 0 | 94 | 561 | 2 | 454 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 2wtx_D | 0 | 94 | 561 | 3 | 455 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| ES813375 | 373 | 454 | 826 | 0 |
| FY441598 | 284 | 562 | 841 | 0 |
| FY436130 | 274 | 561 | 834 | 0 |
| CX546763 | 316 | 68 | 382 | 0 |
| CV711307 | 294 | 91 | 383 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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