| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra008926 |
| Family | GH32 |
| Protein Properties | Length: 516 Molecular Weight: 57798 Isoelectric Point: 4.7431 |
| Chromosome | Chromosome/Scaffold: 10 Start: 14449427 End: 14451310 |
| Description | 6-&1-fructan exohydrolase |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 1 | 308 | 0 |
| MYYKGFYHMFYQHNNLAPRFSEIMIWGHSVSQDMVNWIQLEPAFVPTESFDRHSCWSGSATILPDGKPVILYTGLEEHEELDRRQVTVLAEPKDASDPLL REWVKPKNNPVMLPPHDVPHDCFRDPTTGWQGQDGIWRVIVGAKEINTERGMAVLYRSKDFVEWTKYPTPLLATQDTGMWECPDFFPVSLTGKEGVETSV NNASVKHVLKVSFGGHDCYVIGTYSSESEDFAADSEFTNTTADLRYDHGTFYASKAFFDSVKNRRINWGWVIETDSVEDDLEKGWSGLLSLPREMWLDTS GKRLIQWP | |||
| Full Sequence |
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| Protein Sequence Length: 516 Download |
| MYYKGFYHMF YQHNNLAPRF SEIMIWGHSV SQDMVNWIQL EPAFVPTESF DRHSCWSGSA 60 TILPDGKPVI LYTGLEEHEE LDRRQVTVLA EPKDASDPLL REWVKPKNNP VMLPPHDVPH 120 DCFRDPTTGW QGQDGIWRVI VGAKEINTER GMAVLYRSKD FVEWTKYPTP LLATQDTGMW 180 ECPDFFPVSL TGKEGVETSV NNASVKHVLK VSFGGHDCYV IGTYSSESED FAADSEFTNT 240 TADLRYDHGT FYASKAFFDS VKNRRINWGW VIETDSVEDD LEKGWSGLLS LPREMWLDTS 300 GKRLIQWPIE EINYLRTKQV SLDNTHLAGC SILEISGITA AQADVEVTFD LPVLEGNPQV 360 LDSDHVDDAV LFDRDSSVGC VYGPFGLLAL ATSDLSEQTA IFFKIIRRGN GYSVVMGSDE 420 NKSSLRDNAR KSAHGTVLDI DPLHEKISLR CLIDHSIIES YGAGGRNVIT SRVYPKVAIG 480 EAAKLYLFND GTRGVNISSM EAWSMRNAEV NLNAL* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 5.0e-40 | 2 | 476 | 492 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 4.0e-49 | 1 | 477 | 484 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 5.0e-76 | 1 | 311 | 313 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 3.0e-113 | 1 | 308 | 317 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 3.0e-137 | 1 | 465 | 474 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1ST8 | 0 | 1 | 509 | 27 | 532 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
| EMBL | CAB87665.1 | 0 | 1 | 513 | 33 | 544 | fructosidase-like protein [Arabidopsis thaliana] |
| RefSeq | NP_001078574.1 | 0 | 1 | 376 | 36 | 410 | AtcwINV6 (6-&1-fructan exohydrolase); hydrolase, hydrolyzing O-glycosyl compounds / inulinase/ levanase [Arabidopsis thaliana] |
| RefSeq | NP_568254.1 | 0 | 1 | 513 | 36 | 547 | AtcwINV6 (6-&1-fructan exohydrolase); hydrolase, hydrolyzing O-glycosyl compounds / inulinase/ levanase [Arabidopsis thaliana] |
| RefSeq | XP_002309497.1 | 0 | 1 | 511 | 63 | 567 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2aey_A | 0 | 1 | 509 | 27 | 532 | A Chain A, Native Cytokinin Dehydrogenase |
| PDB | 2ade_A | 0 | 1 | 509 | 27 | 532 | A Chain A, Native Cytokinin Dehydrogenase |
| PDB | 2add_A | 0 | 1 | 509 | 27 | 532 | A Chain A, Native Cytokinin Dehydrogenase |
| PDB | 1st8_A | 0 | 1 | 509 | 27 | 532 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
| PDB | 2aez_A | 0 | 1 | 509 | 27 | 532 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| fructan degradation | RXN-1841 | EC-3.2.1.80 | fructan β-fructosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CT842376 | 525 | 1 | 511 | 0 |
| FD949297 | 182 | 1 | 182 | 0 |
| FD956604 | 219 | 245 | 463 | 0 |
| FD952457 | 214 | 303 | 516 | 0 |
| EE591202 | 415 | 106 | 511 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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