Basic Information | |
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Species | Brassica rapa |
Cazyme ID | Bra009448 |
Family | GT64 |
Protein Properties | Length: 764 Molecular Weight: 86005 Isoelectric Point: 8.8322 |
Chromosome | Chromosome/Scaffold: 10 Start: 16684412 End: 16687191 |
Description | glycosyltransferase family protein 47 |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT64 | 516 | 750 | 0 |
FTLATMTYDARLWNLKMYVKHYSRCASVKEIVVIWNKGHPPDPTELDSAVPVRIRVEKRNSLNNRFSIDPLITTRAVLELDDDIMMSCDEIEKGFRVWRE HPERLVGFYPRYVDQSMTYNAEKFARSHKGYNMILTGAAFMDVGFAFGLYQSERARLGREFVDEQFNCEDVLLNFLYANASGSAKAVEYVRPSALVLDTS KFSGVAISGNTDQHYRKRSECVRRFSELYGSLSDR |
Full Sequence |
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Protein Sequence Length: 764 Download |
MMEETAEIVG KSSLHKNVHN HNRYRYLSSY GGSSSVRRFL FFVSCFGLYA LVALTYAWLA 60 FPPHIGRTYH VSSSSLGCRE DNEGSWSVGV YYGDSPFTLK PIETVNVWRN ESAAWPVANP 120 VLTCASLTNS GFPSNFIADP FLYVQGDTLY LFFENKNPIT LQGDIGVAES TDKGATWKPL 180 GIALDEPWHL SFPFVFNFNG QIYMMPESSQ IGELRLYRAI NFPLTWKLEK VILQKPLVDT 240 TIIHHQGNYW LLGSDHSSFG AKKNGQLEIW YSTTPLGPWK QHKNNPIYNG KRNVGARNGG 300 RGFLHDGNLY RVGQDCGENY GKRIRMFKIE TLSKEEYIEV EVPFDLEVPR KGKNSWNGLR 360 HHHFEVRQLS SGGYVGLVDG DRVTSGDLFH RVLLGYTSLA GAITLVVLLG FLLGVVNCIV 420 PSTWCMNYYA GKRTDALFNL ETDGFFSGKL RRMCSRLNRV PPFLRGLVKP SSALGRLSLA 480 LILLVGVVLS CLGVSYIYGG SGAVQPYTFK GHASQFTLAT MTYDARLWNL KMYVKHYSRC 540 ASVKEIVVIW NKGHPPDPTE LDSAVPVRIR VEKRNSLNNR FSIDPLITTR AVLELDDDIM 600 MSCDEIEKGF RVWREHPERL VGFYPRYVDQ SMTYNAEKFA RSHKGYNMIL TGAAFMDVGF 660 AFGLYQSERA RLGREFVDEQ FNCEDVLLNF LYANASGSAK AVEYVRPSAL VLDTSKFSGV 720 AISGNTDQHY RKRSECVRRF SELYGSLSDR RGEFGGRKDG GDL* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd08978 | GH_F | 0.0007 | 105 | 299 | 211 | + Glycosyl hydrolase families 43 and 62 form CAZY clan GH-F. This glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) includes family 43 (GH43) and 62 (GH62). GH43 includes enzymes with beta-xylosidase (EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanases (beta-xylanases) and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. GH62 includes enzymes characterized as arabinofuranosidases (alpha-L-arabinofuranosidases; EC 3.2.1.55) that specifically cleave either alpha-1,2 or alpha-1,3-L-arabinofuranose side chains from xylans. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. GH62 are also predicted to be inverting enzymes. A common structural feature of both, GH43 and GH62 enzymes, is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam09258 | Glyco_transf_64 | 2.0e-78 | 516 | 754 | 247 | + Glycosyl transferase family 64 domain. Members of this family catalyze the transfer reaction of N-acetylglucosamine and N-acetylgalactosamine from the respective UDP-sugars to the non-reducing end of [glucuronic acid]beta 1-3[galactose]beta 1-O-naphthalenemethanol, an acceptor substrate analog of the natural common linker of various glycosylaminoglycans. They are also required for the biosynthesis of heparan-sulphate. |
Gene Ontology | |
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GO Term | Description |
GO:0016758 | transferase activity, transferring hexosyl groups |
GO:0031227 | intrinsic to endoplasmic reticulum membrane |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAB85556.1 | 0 | 2 | 763 | 1 | 764 | putative protein [Arabidopsis thaliana] |
RefSeq | NP_196070.2 | 0 | 1 | 763 | 1 | 765 | glycosyltransferase family protein 47 [Arabidopsis thaliana] |
RefSeq | XP_002262646.1 | 0 | 74 | 763 | 69 | 756 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002315348.1 | 0 | 71 | 763 | 95 | 847 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002532924.1 | 0 | 74 | 763 | 17 | 704 | transferase, transferring glycosyl groups, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1on8_B | 2e-26 | 510 | 747 | 24 | 270 | A Chain A, Crystal Structure Of Chondroitin Polymerase From Escherichia Coli Strain K4 (K4cp) Complexed With Udp-Glcua And Udp |
PDB | 1on8_A | 2e-26 | 510 | 747 | 24 | 270 | A Chain A, Crystal Structure Of Chondroitin Polymerase From Escherichia Coli Strain K4 (K4cp) Complexed With Udp-Glcua And Udp |
PDB | 1on6_B | 2e-26 | 510 | 747 | 24 | 270 | A Chain A, Crystal Structure Of Chondroitin Polymerase From Escherichia Coli Strain K4 (K4cp) Complexed With Udp-Glcua And Udp |
PDB | 1on6_A | 2e-26 | 510 | 747 | 24 | 270 | A Chain A, Crystal Structure Of Chondroitin Polymerase From Escherichia Coli Strain K4 (K4cp) Complexed With Udp-Glcua And Udp |
PDB | 1omz_B | 2e-26 | 510 | 747 | 24 | 270 | A Chain A, Crystal Structure Of Chondroitin Polymerase From Escherichia Coli Strain K4 (K4cp) Complexed With Udp-Glcua And Udp |