| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra009521 |
| Family | GH13 |
| Protein Properties | Length: 852 Molecular Weight: 96971.3 Isoelectric Point: 5.2555 |
| Chromosome | Chromosome/Scaffold: 10 Start: 17062064 End: 17067516 |
| Description | starch branching enzyme 2.2 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 354 | 675 | 5.9e-29 |
| LPRIKKLGYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRCGTPEDLKSLIDRAHELGLIVLMDIVHSHASKNTLDGLNMFDGTDAHYFHSGPRGYHWMWD SRLFNYGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLSVGFTGNYTEYFGLETDVDAVTYLMLVNDMIHGLYPEAVTVGEDVSGMPTFCIP VQDGGVGFDYRLHMAIADKWIEILKKRDEDWKMGDIIYTLTNRRWSEKCIAYAESHDQALVGDKTIAFWLMDKDMYDFMAVDRPSTPLVDRGIALHKMIR LITMGLGGEGYLNFMGNEFGHP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 852 Download |
| MVVVHGVSLT PGVLVSSFLP LPLSSSNARS LNSTVSFFLK KRPLSRKIFA GNQSAKFDSK 60 SQAVSKKVLV PDNVDDGASL VTDDALQGPS SVSEESQILN LESQTMEEDG IVITEDQASV 120 SVNSSKVVKE KEVKPRTVPP PGNGQIIYEI DPMLRSYKDH LDYRYKQYKR LREEIDKYEG 180 GLEAFSRGYE KLGFLRSDAG ITYREWAPGA KSASLIGDFN NWNPNADIMT RNEFGVWEIF 240 LPNNTDGSPA IPHGSRVKIR MDTPSGIKDS IPAWIKFSVQ APGEIPFNGI YYDPPEEEKY 300 VFKHPQPKRP KSLRIYESHV GMSSTDTFHC DDDSELVMQE PMINTYANFR DDVLPRIKKL 360 GYNAVQIMAI QEHSYYASFG YHVTNFFAPS SRCGTPEDLK SLIDRAHELG LIVLMDIVHS 420 HASKNTLDGL NMFDGTDAHY FHSGPRGYHW MWDSRLFNYG SWEVLRYLLS NARWWLEEYK 480 FDGFRFDGVT SMMYTHHGLS VGFTGNYTEY FGLETDVDAV TYLMLVNDMI HGLYPEAVTV 540 GEDVSGMPTF CIPVQDGGVG FDYRLHMAIA DKWIEILKKR DEDWKMGDII YTLTNRRWSE 600 KCIAYAESHD QALVGDKTIA FWLMDKDMYD FMAVDRPSTP LVDRGIALHK MIRLITMGLG 660 GEGYLNFMGN EFGHPEWIDF PRGEQRLSDG SVIPGNNFSY DKCRRRFDLG DADYLRYHGL 720 QEFDRAMQHL EEKYGFMTSE HQFISRKDEG DRVIVFERGD LVFVFNFHWT SSYFDYRIGC 780 SKPGKYKIVL DSDDPLFGGF GRLDRTAEYF TFEGSFDGRP SSFMVYTPCR TAVVYALADR 840 DDDLETLGSK P* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02960 | PLN02960 | 2.0e-11 | 155 | 241 | 93 | + alpha-amylase | ||
| PLN03244 | PLN03244 | 6.0e-136 | 249 | 838 | 598 | + alpha-amylase; Provisional | ||
| PLN02447 | PLN02447 | 0 | 63 | 843 | 785 | + 1,4-alpha-glucan-branching enzyme | ||
| cd11321 | AmyAc_bac_euk_BE | 0 | 296 | 725 | 431 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02960 | PLN02960 | 0 | 249 | 838 | 594 | + alpha-amylase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| GO:0043169 | cation binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAB03100.1 | 0 | 8 | 841 | 3 | 800 | starch branching enzyme class II [Arabidopsis thaliana] |
| GenBank | ABO31358.1 | 0 | 29 | 839 | 27 | 840 | starch branching enzyme II-1 [Malus x domestica] |
| GenBank | ABO31359.1 | 0 | 24 | 839 | 21 | 840 | starch branching enzyme II-2 [Malus x domestica] |
| RefSeq | NP_195985.3 | 0 | 1 | 841 | 1 | 805 | SBE2.2 (starch branching enzyme 2.2); 1,4-alpha-glucan branching enzyme [Arabidopsis thaliana] |
| RefSeq | XP_002534111.1 | 0 | 3 | 839 | 5 | 838 | starch branching enzyme II, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3amk_A | 0 | 147 | 841 | 13 | 696 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3vu2_B | 0 | 147 | 841 | 13 | 696 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3vu2_A | 0 | 147 | 841 | 13 | 696 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3aml_A | 0 | 147 | 841 | 13 | 696 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3k1d_A | 0 | 199 | 833 | 136 | 717 | A Chain A, Crystal Structure Of Glycogen Branching Enzyme Synonym: 1,4- Glucan:1,4-Alpha-D-Glucan 6-Glucosyl-Transferase From Mycob Tuberculosis H3 |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch biosynthesis | RXN-7710 | EC-2.4.1.18 | 1,4-α-glucan branching enzyme |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO794536 | 705 | 141 | 845 | 0 |
| HO777638 | 651 | 195 | 845 | 0 |
| HO458123 | 393 | 444 | 836 | 0 |
| HO458123 | 308 | 141 | 443 | 0 |
| HO777638 | 47 | 150 | 196 | 0.0000000007 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
