| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra009589 |
| Family | GT4 |
| Protein Properties | Length: 430 Molecular Weight: 48358.4 Isoelectric Point: 7.5534 |
| Chromosome | Chromosome/Scaffold: 10 Start: 17339613 End: 17341557 |
| Description | UDP-Glycosyltransferase superfamily protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT4 | 201 | 335 | 2.5e-32 |
| ITIVVISRLVYRKGADLLVEVIPEVCRLYPNVRFVVGGDGPKHVRLEEMREKHSLQDRVEMLGAVPHSRVRYVLVTGGIFLNSSLTEAFCIAILEAASCG LLTVSTRVGGVLPDDMVVLAEPDPDDMVRAIEKAI | |||
| Full Sequence |
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| Protein Sequence Length: 430 Download |
| MAEPKLRILM VSDFFFPNFG GVENHIYYLS QCLLNLGHKV VVMTHAYGNR TGVRYMTGGL 60 KVYYVPWRPF TMQNTFPTVY GTLPIVRTIL KRERITVVHG HQAFSTLCHE ALMHARTMGY 120 RVVFTDHSLY GFADVGSIHM NKVLQFTLAD IDQAICVSHT SKENTVLRSG LAPGKVFMIP 180 NAVDTAMFKP ADVRPSSDDV ITIVVISRLV YRKGADLLVE VIPEVCRLYP NVRFVVGGDG 240 PKHVRLEEMR EKHSLQDRVE MLGAVPHSRV RYVLVTGGIF LNSSLTEAFC IAILEAASCG 300 LLTVSTRVGG VLPDDMVVLA EPDPDDMVRA IEKAISILPS INPEEMHNRM TKLYSWQDVA 360 KRTEIVYDRA LKCSNRSLLE RLSRFLSCGA WAGKVFCMVM IIDYLLWRLL QLLQPDDDIE 420 EAPEINFCN* 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG0438 | RfaG | 7.0e-42 | 7 | 372 | 386 | + Glycosyltransferase [Cell envelope biogenesis, outer membrane] | ||
| pfam08288 | PIGA | 5.0e-52 | 45 | 134 | 90 | + PIGA (GPI anchor biosynthesis). This domain is found on phosphatidylinositol n-acetylglucosaminyltransferase proteins. These proteins are involved in GPI anchor biosynthesis and are associated with disease the paroxysmal nocturnal haemoglobinuria. | ||
| cd03798 | GT1_wlbH_like | 6.0e-54 | 8 | 370 | 379 | + This family is most closely related to the GT1 family of glycosyltransferases. wlbH in Bordetella parapertussis has been shown to be required for the biosynthesis of a trisaccharide that, when attached to the B. pertussis lipopolysaccharide (LPS) core (band B), generates band A LPS. | ||
| cd03801 | GT1_YqgM_like | 5.0e-68 | 7 | 367 | 383 | + This family is most closely related to the GT1 family of glycosyltransferases and named after YqgM in Bacillus licheniformis about which little is known. Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea. | ||
| cd03796 | GT1_PIG-A_like | 0 | 7 | 400 | 399 | + This family is most closely related to the GT1 family of glycosyltransferases. Phosphatidylinositol glycan-class A (PIG-A), an X-linked gene in humans, is necessary for the synthesis of N-acetylglucosaminyl-phosphatidylinositol, a very early intermediate in glycosyl phosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is an important cellular structure that facilitates the attachment of many proteins to cell surfaces. Somatic mutations in PIG-A have been associated with Paroxysmal Nocturnal Hemoglobinuria (PNH), an acquired hematological disorder. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0006506 | GPI anchor biosynthetic process |
| GO:0009058 | biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAB72148.1 | 0 | 1 | 429 | 1 | 435 | n-acetylglucosaminyl-phosphatidylinositol-like protein [Arabidopsis thaliana] |
| RefSeq | NP_566874.1 | 0 | 1 | 429 | 1 | 432 | SETH2; transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002323038.1 | 0 | 7 | 412 | 2 | 432 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002461824.1 | 0 | 5 | 426 | 7 | 430 | hypothetical protein SORBIDRAFT_02g008800 [Sorghum bicolor] |
| RefSeq | XP_002529442.1 | 0 | 1 | 429 | 1 | 431 | glycosyltransferase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2gek_A | 0.0000003 | 6 | 306 | 21 | 313 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 Complexed With Udp-Glucose |
| PDB | 2gej_A | 0.0000003 | 6 | 306 | 21 | 313 | A Chain A, Crystal Structure Of Phosphatidylinositol Mannosyltransferase (Pima) From Mycobacterium Smegmatis In Complex With Gdp-Man |
| PDB | 3okp_A | 0.000001 | 7 | 327 | 6 | 334 | A Chain A, Crystal Structure Of Phosphatidylinositol Mannosyltransferase (Pima) From Mycobacterium Smegmatis In Complex With Gdp-Man |
| PDB | 3okc_A | 0.000001 | 7 | 327 | 6 | 334 | A Chain A, Crystal Structure Of Corynebacterium Glutamicum Pimb' Bound To Gdp (Orthorhombic Crystal Form) |
| PDB | 3oka_B | 0.000001 | 7 | 327 | 6 | 334 | A Chain A, Crystal Structure Of Corynebacterium Glutamicum Pimb' In Complex With Gdp-Man (Triclinic Crystal Form) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EE592228 | 219 | 5 | 223 | 0 |
| EE592228 | 213 | 217 | 426 | 0 |
| DK461907 | 257 | 1 | 257 | 0 |
| DY295948 | 313 | 1 | 313 | 0 |
| DK498787 | 244 | 1 | 244 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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