| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra011802 |
| Family | PL4 |
| Protein Properties | Length: 665 Molecular Weight: 75984.5 Isoelectric Point: 9.5218 |
| Chromosome | Chromosome/Scaffold: 01 Start: 440202 End: 443720 |
| Description | Rhamnogalacturonate lyase family protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| PL4 | 49 | 645 | 0 |
| AREVIVDNGIIRVSFSSPQGLITSIKYKGIDNVLNPHVRTRGYWDITWQGENIRGLDGIEGTNFRVITQNEEQVEISFSRKWNGGSKHIPLNIDKRYIIR TNTSGIYAYGVFERLSEWPEVEMGQVRIVFKLNTDRFRYMVVADDRQRQMPTDNDRDIHRGHAKALAYKEAVQLTNPADLRFKNQVDDKYQYSCEVKDNK VHGWISTKSHVGFWLISPSGEYRSGGPSKQELTSHVGPTAISSFTTGHYVGRDMETTYKTGEAWKKVFGPVFIYLNSDSTGNNPRHSLWEDAKRQTEEEV EAWPYDFVASSDFPSRQERGTVTGRLLVNDRFLTPAQSAYIGLAPPGEAGSWQTNTKGYQFWTQTNETGYFTIDNVRPGTYNLYGWVPGFIGDFQYQNRV NVAAGSEISLDRVVFKPPRNGPTLWEIGVPDRTAREYFVPEPYKDTMNPLYLNHTDKFRQYGLWQRYTELYPNHDLVYTIGVSNYSQNWFYAHVTRNIDK STYVPTTWQIVFQLPYVNRQGSYTLQLALASAARANLQVRFNNEYSRPLFSTGNIGKDNAIARHGIHGVYRLYSVNVPGRLLRTGTNTIYLRQAKAT | |||
| Full Sequence |
|---|
| Protein Sequence Length: 665 Download |
| MILQKIASSS MICFLLLLLP IAFSQPTRNL DGISARAPTV QQIRNRHGAR EVIVDNGIIR 60 VSFSSPQGLI TSIKYKGIDN VLNPHVRTRG YWDITWQGEN IRGLDGIEGT NFRVITQNEE 120 QVEISFSRKW NGGSKHIPLN IDKRYIIRTN TSGIYAYGVF ERLSEWPEVE MGQVRIVFKL 180 NTDRFRYMVV ADDRQRQMPT DNDRDIHRGH AKALAYKEAV QLTNPADLRF KNQVDDKYQY 240 SCEVKDNKVH GWISTKSHVG FWLISPSGEY RSGGPSKQEL TSHVGPTAIS SFTTGHYVGR 300 DMETTYKTGE AWKKVFGPVF IYLNSDSTGN NPRHSLWEDA KRQTEEEVEA WPYDFVASSD 360 FPSRQERGTV TGRLLVNDRF LTPAQSAYIG LAPPGEAGSW QTNTKGYQFW TQTNETGYFT 420 IDNVRPGTYN LYGWVPGFIG DFQYQNRVNV AAGSEISLDR VVFKPPRNGP TLWEIGVPDR 480 TAREYFVPEP YKDTMNPLYL NHTDKFRQYG LWQRYTELYP NHDLVYTIGV SNYSQNWFYA 540 HVTRNIDKST YVPTTWQIVF QLPYVNRQGS YTLQLALASA ARANLQVRFN NEYSRPLFST 600 GNIGKDNAIA RHGIHGVYRL YSVNVPGRLL RTGTNTIYLR QAKATGPFEG LMYDYIRLEA 660 ISRA* 720 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam13620 | CarboxypepD_reg | 0.0002 | 411 | 459 | 49 | + Carboxypeptidase regulatory-like domain. | ||
| cd10316 | RGL4_M | 8.0e-34 | 366 | 458 | 93 | + Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10317 | RGL4_C | 4.0e-49 | 473 | 659 | 189 | + C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10320 | RGL4_N | 3.0e-69 | 43 | 328 | 293 | + N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| pfam06045 | Rhamnogal_lyase | 1.0e-83 | 15 | 234 | 224 | + Rhamnogalacturonate lyase family. Rhamnogalacturonate lyase (EC:4.2.2.-) degrades the rhamnogalacturonan I (RG-I) backbone of pectin. This family contains mainly members from plants, but also contains the plant pathogen Erwinia chrysanthemi. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAS99719.1 | 0 | 24 | 664 | 34 | 677 | At2g22620 [Arabidopsis thaliana] |
| DDBJ | BAD94618.1 | 0 | 157 | 664 | 2 | 510 | LG127/30 like gene [Arabidopsis thaliana] |
| EMBL | CAB37543.1 | 0 | 25 | 664 | 26 | 649 | LG127/30 like gene [Arabidopsis thaliana] |
| RefSeq | NP_179847.1 | 0 | 24 | 664 | 34 | 677 | lyase [Arabidopsis thaliana] |
| RefSeq | NP_195516.2 | 0 | 25 | 664 | 26 | 667 | lyase [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3njx_A | 0.008 | 408 | 639 | 283 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus Mutant H210a |
| PDB | 3njv_A | 0.009 | 408 | 639 | 283 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus Mutant H210a |
| PDB | 2xhn_B | 0.009 | 408 | 639 | 283 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
| PDB | 2xhn_A | 0.009 | 408 | 639 | 283 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
| PDB | 1nkg_A | 0.009 | 408 | 639 | 283 | 483 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus |
