| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra012548 |
| Family | AA3 |
| Protein Properties | Length: 771 Molecular Weight: 84690.7 Isoelectric Point: 7.8825 |
| Chromosome | Chromosome/Scaffold: 03 Start: 23643396 End: 23647199 |
| Description | Long-chain fatty alcohol dehydrogenase family protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA3 | 225 | 756 | 0 |
| GIINLKKPREAVTKKLTDHGFTVSTQKSNNNRSSISNPVMTIRCDAVVVGSGSGGGVVAGLLAKAGFKVLVVEKGNYYARSNLSQLEGQAMDEMYLSGGL LATSDMNVVILAGSTVGGGSTINWSASIKTPEHVMKEWAEKSELELFESDLYREAMDVVCERMGVQCGFVEEGFNNEVLRKGCEELGLKVKNIPRNASKD HYCGFCSLGCKKGQKQGTSETWLVDLVESGNGSILPGCKAMEVLYECEGGNKKKTSTGIAFAFGEDIYVVESRATVVACGALRTPHLLKRSGLKNGNIGR NLCLHPVVMAWGWFPEEEKWPEKKKKSYEGGIMTAMSSVLNAEAKTTYGETVIQTPSLHPGMFSGVIPWTSSNDFKTRMLKFSRTAHVFTLLRDKGTGTI NSKSYIDYNLNDEDEESLKKGLESVLNILIAAGAEEIGTHNSKGKSLNVRTASAVEIERFVREESSKSLKDLSGQICSAHQMGSCRMGIRPEESAVRPMG ETWEVEGLFVADTSVFPTALGVNPMVTVQSIA | |||
| Full Sequence |
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| Protein Sequence Length: 771 Download |
| MEYQNKSSLH KDSTEAKLDV EFGSPRQQDE TKTLYANSLT AREMESLVAI CDTLIPSIDG 60 AEVGHLDDGV AGYYSASASH TGTPDRVARL MSQRLHHPRK WILRVGLWSL STWIGSLVLC 120 GRRSFIGGFP FFQRFCRLPK KQREETLLNW ASSYFLLLRM FFRSIKLITA LVFFTQVDEK 180 GKNLAWKAIG YNGPSSDHSD HEEELNETKT NKEMPKEIFG PLYNGIINLK KPREAVTKKL 240 TDHGFTVSTQ KSNNNRSSIS NPVMTIRCDA VVVGSGSGGG VVAGLLAKAG FKVLVVEKGN 300 YYARSNLSQL EGQAMDEMYL SGGLLATSDM NVVILAGSTV GGGSTINWSA SIKTPEHVMK 360 EWAEKSELEL FESDLYREAM DVVCERMGVQ CGFVEEGFNN EVLRKGCEEL GLKVKNIPRN 420 ASKDHYCGFC SLGCKKGQKQ GTSETWLVDL VESGNGSILP GCKAMEVLYE CEGGNKKKTS 480 TGIAFAFGED IYVVESRATV VACGALRTPH LLKRSGLKNG NIGRNLCLHP VVMAWGWFPE 540 EEKWPEKKKK SYEGGIMTAM SSVLNAEAKT TYGETVIQTP SLHPGMFSGV IPWTSSNDFK 600 TRMLKFSRTA HVFTLLRDKG TGTINSKSYI DYNLNDEDEE SLKKGLESVL NILIAAGAEE 660 IGTHNSKGKS LNVRTASAVE IERFVREESS KSLKDLSGQI CSAHQMGSCR MGIRPEESAV 720 RPMGETWEVE GLFVADTSVF PTALGVNPMV TVQSIAYCIG LNVVGALKKK * 780 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR03970 | Rv0697 | 0.001 | 681 | 756 | 81 | + dehydrogenase, Rv0697 family. This model describes a set of dehydrogenases belonging to the glucose-methanol-choline oxidoreductase (GMC oxidoreductase) family. Members of the present family are restricted to Actinobacterial genome contexts containing also members of families TIGR03962 and TIGR03969 (the mycofactocin system), and are proposed to be uniform in function. | ||
| TIGR01810 | betA | 1.0e-5 | 436 | 741 | 346 | + choline dehydrogenase. Choline dehydrogenase catalyzes the conversion of exogenously supplied choline into the intermediate glycine betaine aldehyde, as part of a two-step oxidative reaction leading to the formation of osmoprotectant betaine. This enzymatic system can be found in both gram-positive and gram-negative bacteria. As in Escherichia coli , Staphylococcus xylosus , and Sinorhizobium meliloti, this enzyme is found associated in a transciptionally co-induced gene cluster with betaine aldehyde dehydrogenase, the second catalytic enzyme in this reaction. Other gram-positive organisms have been shown to employ a different enzymatic system, utlizing a soluable choline oxidase or type III alcohol dehydrogenase instead of choline dehydrogenase. This enzyme is a member of the GMC oxidoreductase family (pfam00732 and pfam05199), sharing a common evoluntionary origin and enzymatic reaction with alcohol dehydrogenase. Outgrouping from this model, Caulobacter crescentus shares sequence homology with choline dehydrogenase, yet other genes participating in this enzymatic reaction have not currently been identified [Cellular processes, Adaptations to atypical conditions]. | ||
| pfam05199 | GMC_oxred_C | 1.0e-21 | 617 | 759 | 150 | + GMC oxidoreductase. This domain found associated with pfam00732. | ||
| COG2303 | BetA | 5.0e-28 | 312 | 757 | 501 | + Choline dehydrogenase and related flavoproteins [Amino acid transport and metabolism] | ||
| pfam00732 | GMC_oxred_N | 3.0e-78 | 315 | 531 | 223 | + GMC oxidoreductase. This family of proteins bind FAD as a cofactor. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016614 | oxidoreductase activity, acting on CH-OH group of donors |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAA18625.1 | 5e-26 | 4 | 87 | 2 | 86 | putative protein [Arabidopsis thaliana] |
| EMBL | CAA18625.1 | 0 | 176 | 770 | 85 | 677 | putative protein [Arabidopsis thaliana] |
| RefSeq | NP_193673.2 | 0 | 44 | 770 | 1 | 725 | alcohol oxidase-related [Arabidopsis thaliana] |
| RefSeq | XP_002269629.1 | 0 | 14 | 770 | 16 | 786 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002328753.1 | 0 | 44 | 770 | 1 | 744 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ljp_B | 0.0000000008 | 403 | 755 | 170 | 518 | A Chain A, Crystal Structure Of Choline Oxidase V464a Mutant |
| PDB | 3ljp_A | 0.0000000008 | 403 | 755 | 170 | 518 | A Chain A, Crystal Structure Of Choline Oxidase V464a Mutant |
| PDB | 3nne_H | 0.000000001 | 403 | 755 | 170 | 518 | A Chain A, Crystal Structure Of Choline Oxidase S101a Mutant |
| PDB | 3nne_G | 0.000000001 | 403 | 755 | 170 | 518 | A Chain A, Crystal Structure Of Choline Oxidase S101a Mutant |
| PDB | 3nne_F | 0.000000001 | 403 | 755 | 170 | 518 | A Chain A, Crystal Structure Of Choline Oxidase S101a Mutant |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DC883850 | 463 | 315 | 770 | 0 |
| DK515004 | 250 | 20 | 269 | 0 |
| HO794753 | 751 | 37 | 767 | 0 |
| BX836801 | 285 | 432 | 716 | 0 |
| BX836801 | 51 | 717 | 767 | 1e-18 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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