| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra013391 |
| Family | AA3 |
| Protein Properties | Length: 509 Molecular Weight: 55077 Isoelectric Point: 6.5773 |
| Chromosome | Chromosome/Scaffold: 01 Start: 5365170 End: 5366696 |
| Description | Long-chain fatty alcohol dehydrogenase family protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA3 | 1 | 493 | 3.36312e-44 |
| MTIQCDAVVVGSGSGGGVVAGMLAKAGYKVLVVEKGNYYARSNLSLHEGQALDEMYLSGGLLATTDMDVVILAGSTVGGGSTINWSASIKTPEHVMKEWS EKSELEMFGSHLYREAMDVVCERMGVQCEFLEEGFNNEILRKGCENLGLPVKNIPRNAPSDHYCGFCCLGCKKGQKQGTSETWLVDLVESGNGLILPGCE AMEVLYECKGGKKTKTSTGIAFAFGKDIYMVESRATIVACGALRTPHLLKHSGLKNGSIGRNLCLHPVVMAWGWFPEEEEWPEKKKRSYQGGIMTAMSSV VNEETKNSYGETVIQTPSLHPGLFSGVIPWTSSKEFKSRMLKFSRTAHVFTLLRDKGTGTINSKSSIEYNLNNEDEESLKKGLERVLNILTAAGAEEIGT HNSEGKSLNVKTTSAVEIERFVREESSKSLKDLSGQICSAHQMGSCRMGTSPEVSAVKPTGETWEVEGLFVADTSVFPTALGVNPMVTVQSIA | |||
| Full Sequence |
|---|
| Protein Sequence Length: 509 Download |
| MTIQCDAVVV GSGSGGGVVA GMLAKAGYKV LVVEKGNYYA RSNLSLHEGQ ALDEMYLSGG 60 LLATTDMDVV ILAGSTVGGG STINWSASIK TPEHVMKEWS EKSELEMFGS HLYREAMDVV 120 CERMGVQCEF LEEGFNNEIL RKGCENLGLP VKNIPRNAPS DHYCGFCCLG CKKGQKQGTS 180 ETWLVDLVES GNGLILPGCE AMEVLYECKG GKKTKTSTGI AFAFGKDIYM VESRATIVAC 240 GALRTPHLLK HSGLKNGSIG RNLCLHPVVM AWGWFPEEEE WPEKKKRSYQ GGIMTAMSSV 300 VNEETKNSYG ETVIQTPSLH PGLFSGVIPW TSSKEFKSRM LKFSRTAHVF TLLRDKGTGT 360 INSKSSIEYN LNNEDEESLK KGLERVLNIL TAAGAEEIGT HNSEGKSLNV KTTSAVEIER 420 FVREESSKSL KDLSGQICSA HQMGSCRMGT SPEVSAVKPT GETWEVEGLF VADTSVFPTA 480 LGVNPMVTVQ SIAYCIGLNV VDALKRKK* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01810 | betA | 0.0002 | 417 | 478 | 63 | + choline dehydrogenase. Choline dehydrogenase catalyzes the conversion of exogenously supplied choline into the intermediate glycine betaine aldehyde, as part of a two-step oxidative reaction leading to the formation of osmoprotectant betaine. This enzymatic system can be found in both gram-positive and gram-negative bacteria. As in Escherichia coli , Staphylococcus xylosus , and Sinorhizobium meliloti, this enzyme is found associated in a transciptionally co-induced gene cluster with betaine aldehyde dehydrogenase, the second catalytic enzyme in this reaction. Other gram-positive organisms have been shown to employ a different enzymatic system, utlizing a soluable choline oxidase or type III alcohol dehydrogenase instead of choline dehydrogenase. This enzyme is a member of the GMC oxidoreductase family (pfam00732 and pfam05199), sharing a common evoluntionary origin and enzymatic reaction with alcohol dehydrogenase. Outgrouping from this model, Caulobacter crescentus shares sequence homology with choline dehydrogenase, yet other genes participating in this enzymatic reaction have not currently been identified [Cellular processes, Adaptations to atypical conditions]. | ||
| PRK02106 | PRK02106 | 3.0e-5 | 417 | 479 | 64 | + choline dehydrogenase; Validated | ||
| pfam05199 | GMC_oxred_C | 6.0e-23 | 354 | 496 | 150 | + GMC oxidoreductase. This domain found associated with pfam00732. | ||
| COG2303 | BetA | 8.0e-26 | 23 | 494 | 526 | + Choline dehydrogenase and related flavoproteins [Amino acid transport and metabolism] | ||
| pfam00732 | GMC_oxred_N | 1.0e-74 | 53 | 268 | 222 | + GMC oxidoreductase. This family of proteins bind FAD as a cofactor. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016614 | oxidoreductase activity, acting on CH-OH group of donors |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAA18625.1 | 0 | 1 | 508 | 172 | 678 | putative protein [Arabidopsis thaliana] |
| RefSeq | NP_193673.2 | 0 | 1 | 508 | 220 | 726 | alcohol oxidase-related [Arabidopsis thaliana] |
| RefSeq | XP_002269629.1 | 0 | 1 | 508 | 271 | 787 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002328753.1 | 0 | 1 | 508 | 231 | 745 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002519938.1 | 0 | 1 | 508 | 203 | 709 | electron carrier, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ljp_B | 0.0000005 | 140 | 492 | 170 | 518 | A Chain A, Crystal Structure Of Choline Oxidase V464a Mutant |
| PDB | 3ljp_A | 0.0000005 | 140 | 492 | 170 | 518 | A Chain A, Crystal Structure Of Choline Oxidase V464a Mutant |
| PDB | 2jbv_B | 0.0000008 | 140 | 492 | 170 | 518 | A Chain A, Crystal Structure Of Choline Oxidase Reveals Insights Into The Catalytic Mechanism |
| PDB | 2jbv_A | 0.0000008 | 140 | 492 | 170 | 518 | A Chain A, Crystal Structure Of Choline Oxidase Reveals Insights Into The Catalytic Mechanism |
| PDB | 3nne_H | 0.0000008 | 140 | 492 | 170 | 518 | A Chain A, Crystal Structure Of Choline Oxidase S101a Mutant |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DC883850 | 462 | 52 | 508 | 0 |
| BX836801 | 308 | 146 | 453 | 0 |
| EV020282 | 219 | 249 | 467 | 0 |
| HO794753 | 519 | 1 | 504 | 0 |
| BX836801 | 50 | 455 | 504 | 9e-20 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
