| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra013734 |
| Family | GH28 |
| Protein Properties | Length: 445 Molecular Weight: 48448.9 Isoelectric Point: 8.0185 |
| Chromosome | Chromosome/Scaffold: 01 Start: 7490454 End: 7493404 |
| Description | Pectin lyase-like superfamily protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH28 | 72 | 416 | 0 |
| RRGGTLLYIPPGVFLTESFNLTSHMTLFLAKGAVLKAVQDTWNWPLIDPLPSYGRGRELPGARYMSFIHGDGLRDVVITGENGTIDGQGEVWWNMWRSRT LKFTRPNLIELKNSKEIIISNVVFQNSPFWNIHPVYCSDVVIHHVTILAPQDSPNTDGIDPDSSSNVCIEDSYISTGDDLVAIKSGWDEYGIAFGRPSSN ITIRRITGSTPFAGIAIGSETSGGIKNIVAEHISLSNMGVGVNIKTNIGRGGYIKNIRISNVYVENARYGIKIAGDTGDHPDAYYNPNALPVVKGIHINN VWGVNVRNAGSIQGLKDSPFTGICLSEINIHGSLNTNRTWKCSDV | |||
| Full Sequence |
|---|
| Protein Sequence Length: 445 Download |
| MWRFSVLILL CSHLAVSSSS LGDSEATCSG IVPLPNRNDR ISITDFGGVG DGRTVNTKAF 60 REAIYRIQHL KRRGGTLLYI PPGVFLTESF NLTSHMTLFL AKGAVLKAVQ DTWNWPLIDP 120 LPSYGRGREL PGARYMSFIH GDGLRDVVIT GENGTIDGQG EVWWNMWRSR TLKFTRPNLI 180 ELKNSKEIII SNVVFQNSPF WNIHPVYCSD VVIHHVTILA PQDSPNTDGI DPDSSSNVCI 240 EDSYISTGDD LVAIKSGWDE YGIAFGRPSS NITIRRITGS TPFAGIAIGS ETSGGIKNIV 300 AEHISLSNMG VGVNIKTNIG RGGYIKNIRI SNVYVENARY GIKIAGDTGD HPDAYYNPNA 360 LPVVKGIHIN NVWGVNVRNA GSIQGLKDSP FTGICLSEIN IHGSLNTNRT WKCSDVIGTS 420 LKVSPWPCSE LRTTGGSYSC SSTF* 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03003 | PLN03003 | 2.0e-25 | 38 | 433 | 419 | + Probable polygalacturonase At3g15720 | ||
| PLN02793 | PLN02793 | 1.0e-26 | 37 | 444 | 435 | + Probable polygalacturonase | ||
| PLN02218 | PLN02218 | 3.0e-30 | 41 | 403 | 376 | + polygalacturonase ADPG | ||
| pfam00295 | Glyco_hydro_28 | 5.0e-32 | 104 | 416 | 319 | + Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes is important in cell wall metabolism. | ||
| COG5434 | PGU1 | 4.0e-51 | 42 | 400 | 394 | + Endopygalactorunase [Cell envelope biogenesis, outer membrane] | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004650 | polygalacturonase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACC91250.1 | 0 | 1 | 444 | 1 | 444 | glycoside hydrolase family 28 protein [Arabidopsis halleri] |
| EMBL | CBI34471.1 | 0 | 1 | 442 | 1 | 445 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_194113.1 | 0 | 1 | 444 | 1 | 444 | glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein [Arabidopsis thaliana] |
| RefSeq | XP_002304552.1 | 0 | 37 | 444 | 4 | 412 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002509949.1 | 0 | 11 | 441 | 12 | 443 | Polygalacturonase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3jur_D | 3.9937e-43 | 34 | 404 | 23 | 413 | A Chain A, The Crystal Structure Of A Hyperthermoactive Exopolygalacturonase From Thermotoga Maritima |
| PDB | 3jur_C | 3.9937e-43 | 34 | 404 | 23 | 413 | A Chain A, The Crystal Structure Of A Hyperthermoactive Exopolygalacturonase From Thermotoga Maritima |
| PDB | 3jur_B | 3.9937e-43 | 34 | 404 | 23 | 413 | A Chain A, The Crystal Structure Of A Hyperthermoactive Exopolygalacturonase From Thermotoga Maritima |
| PDB | 3jur_A | 3.9937e-43 | 34 | 404 | 23 | 413 | A Chain A, The Crystal Structure Of A Hyperthermoactive Exopolygalacturonase From Thermotoga Maritima |
| PDB | 1bhe_A | 7e-22 | 84 | 344 | 51 | 310 | A Chain A, Polygalacturonase From Erwinia Carotovora Ssp. Carotovora |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| homogalacturonan degradation | RXN-2103 | EC-3.2.1.15 | polygalacturonase |
