| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra014080 |
| Family | CE10 |
| Protein Properties | Length: 315 Molecular Weight: 35098.8 Isoelectric Point: 5.5853 |
| Chromosome | Chromosome/Scaffold: 08 Start: 3734190 End: 3737442 |
| Description | alpha/beta-Hydrolases superfamily protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE10 | 37 | 311 | 0 |
| LDPETGVLSKDIIIDPKTGLSARIYRPDSIQPGQKLPVVLYFHGGAFLIASASLPNYHTSINKLVAQANIIAVSVNYRLAPEHPLPTAYEDSWNALQGVQ AGNEPWINDYADFNQFFLAGDSAGANISHHLAFRAKQSNHTVKIKGIGMIHPYFWGTQPVGSEVTDEARKQMVDGWWRFVCPSDKGSDDPWINPFADGSP NLEGLECERVIITVAERDILRERGKMYYEKLVNSKWRGNAEIMETKGKDHVFHIFEPDCDEAKEMVRRLALFINQ | |||
| Full Sequence |
|---|
| Protein Sequence Length: 315 Download |
| MESTKKQVSL EVLPWLVVHS DGTLERLAGT EVCPPGLDPE TGVLSKDIII DPKTGLSARI 60 YRPDSIQPGQ KLPVVLYFHG GAFLIASASL PNYHTSINKL VAQANIIAVS VNYRLAPEHP 120 LPTAYEDSWN ALQGVQAGNE PWINDYADFN QFFLAGDSAG ANISHHLAFR AKQSNHTVKI 180 KGIGMIHPYF WGTQPVGSEV TDEARKQMVD GWWRFVCPSD KGSDDPWINP FADGSPNLEG 240 LECERVIITV AERDILRERG KMYYEKLVNS KWRGNAEIME TKGKDHVFHI FEPDCDEAKE 300 MVRRLALFIN QVEA* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00312 | Esterase_lipase | 1.0e-10 | 60 | 167 | 124 | + Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate. | ||
| COG2272 | PnbA | 2.0e-11 | 55 | 161 | 118 | + Carboxylesterase type B [Lipid metabolism] | ||
| pfam00135 | COesterase | 6.0e-12 | 60 | 117 | 58 | + Carboxylesterase family. | ||
| COG0657 | Aes | 7.0e-31 | 54 | 310 | 263 | + Esterase/lipase [Lipid metabolism] | ||
| pfam07859 | Abhydrolase_3 | 4.0e-56 | 75 | 290 | 220 | + alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM61103.1 | 0 | 1 | 314 | 1 | 314 | unknown [Arabidopsis thaliana] |
| GenBank | ABB89003.1 | 0 | 3 | 311 | 4 | 315 | CXE carboxylesterase [Malus pumila] |
| RefSeq | NP_564507.1 | 0 | 1 | 314 | 1 | 314 | hydrolase [Arabidopsis thaliana] |
| RefSeq | XP_002327124.1 | 0 | 6 | 311 | 5 | 318 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002510251.1 | 0 | 3 | 311 | 2 | 311 | Gibberellin receptor GID1, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2zsi_A | 1e-38 | 38 | 310 | 65 | 349 | A Chain A, Crystal Structure Of Human C3po Complex |
| PDB | 2zsh_A | 1e-38 | 38 | 310 | 65 | 349 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 2o7v_A | 1e-36 | 10 | 236 | 19 | 254 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 2o7r_A | 1e-36 | 10 | 236 | 19 | 254 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ed1_F | 3e-36 | 16 | 310 | 32 | 348 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| formononetin biosynthesis | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| formononetin biosynthesis | RXN-3625 | - | 2,7-dihydroxy-4'-methoxyisoflavanone dehydratase |
| isoflavonoid biosynthesis I | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-3303 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-5502 | - | 2,7,5-trihydroxy-4'-methoxyisoflavanone dehydratase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EE539191 | 248 | 1 | 248 | 0 |
| EW737450 | 246 | 70 | 315 | 0 |
| FD949941 | 253 | 1 | 253 | 0 |
| EX017970 | 224 | 3 | 226 | 0 |
| FD945520 | 228 | 88 | 315 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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