| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra015364 |
| Family | GT4 |
| Protein Properties | Length: 855 Molecular Weight: 95283.7 Isoelectric Point: 6.4393 |
| Chromosome | Chromosome/Scaffold: 10 Start: 2259020 End: 2262762 |
| Description | sucrose phosphate synthase 3F |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT4 | 272 | 440 | 0 |
| TNPHKPMILALSRPDPKKNITTLLKAFGECRHLRELANLTLIMGNRDDIDELPSGNASVLTTALKLIDKYDLYGSVAYPKHHKQSDVPDIYRLAANTKGV FINPALVEPFGLTLIEAAAHGLPMVATKNGGPVDILQALHNGLLVDPHDQEAIANALLKLVSEKNLWNE | |||
| Full Sequence |
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| Protein Sequence Length: 855 Download |
| MPGVYRVDLF TRQICSSEVD WSYAEPTEML TTASAEDCDG DETGESSGAY IIRIPFGPRD 60 KYLPKEILWP YIQEFVDGAL AHILNMSKVL GEQIGNGKPV WPYVIHGHYA DAGDSAALLS 120 GALNVPMVLT GHSLGRNKLE QLLKQGRQSK EDINSTYKIK RRIEAEELSL DAAELVITST 180 RQEIDEQWGL YDGFDVKLEK VLRARARRGV NCHGRYMPRM AVIPPGMDFT NVVVQEETLD 240 GDGELASLAG GAEGSSPKAV PTIWSDVMRF FTNPHKPMIL ALSRPDPKKN ITTLLKAFGE 300 CRHLRELANL TLIMGNRDDI DELPSGNASV LTTALKLIDK YDLYGSVAYP KHHKQSDVPD 360 IYRLAANTKG VFINPALVEP FGLTLIEAAA HGLPMVATKN GGPVDILQAL HNGLLVDPHD 420 QEAIANALLK LVSEKNLWNE CRINGWKNIH LFSWPEHCRT YLTRVASCRM RHPQWQTDAD 480 EMAAQEDEFS LNDSLKDVQD MSLRLSVDGD KPSWNASLEP NSTDPVKQIM SRMKQPETKS 540 KPEVQGKKQG DNVGSRFPVL RRRERLIVIA FDCYNEEGAV DVKSMVKMIQ NIIKAVRSDP 600 KMAKNSGFAL STSMPLDELT SFFKSAKIQV SEFDTLICSS GSEVYYPGAE EGKLLPDPDY 660 ASHIDYRWGN EGLKNTVWKL MNTTAVGGEA RNKGSPSLVE EDKPSSNEHC VAYLIKDRSK 720 VMRIDDLRQK LRLRGLRCHP MYCRNSTRLQ IVPLLASRSQ ALRYLFVRWR LNVANMYVVV 780 GEHGDTDYEE LISGTHKTVI VKGLVTLGSD ALLRSTDLRD DIVPSESPFI GFLKADSPVN 840 EITDILKQLS KAAT* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR02470 | sucr_synth | 8.0e-30 | 49 | 463 | 450 | + sucrose synthase. This model represents sucrose synthase, an enzyme that, despite its name, generally uses rather produces sucrose. Sucrose plus UDP (or ADP) becomes D-fructose plus UDP-glucose (or ADP-glucose), which is then available for cell wall (or starch) biosynthesis. The enzyme is homologous to sucrose phosphate synthase, which catalyzes the penultimate step in sucrose synthesis. Sucrose synthase is found, so far, exclusively in plants and cyanobacteria [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| cd03801 | GT1_YqgM_like | 7.0e-30 | 104 | 462 | 362 | + This family is most closely related to the GT1 family of glycosyltransferases and named after YqgM in Bacillus licheniformis about which little is known. Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea. | ||
| cd03800 | GT1_Sucrose_synthase | 6.0e-122 | 4 | 463 | 461 | + This family is most closely related to the GT1 family of glycosyltransferases. The sucrose-phosphate synthases in this family may be unique to plants and photosynthetic bacteria. This enzyme catalyzes the synthesis of sucrose 6-phosphate from fructose 6-phosphate and uridine 5'-diphosphate-glucose, a key regulatory step of sucrose metabolism. The activity of this enzyme is regulated by phosphorylation and moderated by the concentration of various metabolites and light. | ||
| TIGR02472 | sucr_P_syn_N | 4.0e-131 | 2 | 462 | 462 | + sucrose-phosphate synthase, putative, glycosyltransferase domain. This family consists of the N-terminal regions, or in some cases the entirety, of bacterial proteins closely related to plant sucrose-phosphate synthases (SPS). The C-terminal domain (TIGR02471), found with most members of this family, resembles both bona fide plant sucrose-phosphate phosphatases (SPP) and the SPP-like domain of plant SPS. At least two members of this family lack the SPP-like domain, which may have binding or regulatory rather than enzymatic activity by analogy to plant SPS. This enzyme produces sucrose 6-phosphate and UDP from UDP-glucose and D-fructose 6-phosphate, and may be encoded near the gene for fructokinase. | ||
| TIGR02468 | sucrsPsyn_pln | 0 | 1 | 850 | 859 | + sucrose phosphate synthase/possible sucrose phosphate phosphatase, plant. Members of this family are sucrose-phosphate synthases of plants. This enzyme is known to exist in multigene families in several species of both monocots and dicots. The N-terminal domain is the glucosyltransferase domain. Members of this family also have a variable linker region and a C-terminal domain that resembles sucrose phosphate phosphatase (SPP) (EC 3.1.3.24) (see TIGR01485), the next and final enzyme of sucrose biosynthesis. The SPP-like domain likely serves a binding and not a catalytic function, as the reported SPP is always encoded by a distinct protein. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0009058 | biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAF40445.1 | 0 | 1 | 853 | 211 | 1063 | AC004809_3 Strong similarity to the sucrose-phosphate synthase from Craterostigma plantagineum gb |
| RefSeq | NP_171984.2 | 0 | 1 | 853 | 211 | 1061 | ATSPS3F (sucrose phosphate synthase 3F); sucrose-phosphate synthase/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002298684.1 | 0 | 1 | 853 | 209 | 1066 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002328899.1 | 0 | 1 | 853 | 209 | 1066 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002518055.1 | 0 | 1 | 854 | 207 | 1064 | sucrose phosphate syntase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2r68_A | 0 | 41 | 471 | 73 | 462 | A Chain A, Endopolygalacturonase From The Phytopathogenic Fungus Fusarium Moniliforme |
| PDB | 2r66_A | 0 | 41 | 471 | 73 | 462 | A Chain A, Endopolygalacturonase From The Phytopathogenic Fungus Fusarium Moniliforme |
| PDB | 2r60_A | 0 | 41 | 471 | 73 | 462 | A Chain A, Structure Of Apo Sucrose Phosphate Synthase (Sps) Of Halothermothrix Orenii |
| PDB | 3s29_H | 2e-30 | 25 | 463 | 331 | 764 | A Chain A, Structure Of Apo Sucrose Phosphate Synthase (Sps) Of Halothermothrix Orenii |
| PDB | 3s29_G | 2e-30 | 25 | 463 | 331 | 764 | A Chain A, Structure Of Apo Sucrose Phosphate Synthase (Sps) Of Halothermothrix Orenii |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose biosynthesis | SUCROSE-PHOSPHATE-SYNTHASE-RXN | EC-2.4.1.14 | sucrose-phosphate synthase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO795640 | 518 | 342 | 853 | 0 |
| HO780861 | 367 | 146 | 510 | 0 |
| HO796578 | 372 | 1 | 372 | 0 |
| HO796578 | 84 | 359 | 442 | 7.00649e-45 |
| HO796578 | 27 | 452 | 478 | 7.00649e-45 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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