| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra015873 |
| Family | GH17 |
| Protein Properties | Length: 465 Molecular Weight: 50816.9 Isoelectric Point: 4.9062 |
| Chromosome | Chromosome/Scaffold: 07 Start: 20410124 End: 20419434 |
| Description | beta-1,3-glucanase 2 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH17 | 152 | 463 | 0 |
| IGVCFGQFGSNLPKPSDVVAMYKQYNIRRMRMYGPNPEALDALRGSNIELILDVPNGYLKRLADSQTEANTWVRDNVQKYNDVRFKYISVGNEVMPRGPG GAGTVLIQAMQKIDKALSEAGLSIPVSTTTNMGAFTDTYPPSRGRFSDEYRNFLQPVIGFLVSKRSPLLVNIYTYFGFKNGNFSLDFALFIPSNDEFKDP NNQHYYQNLFDANLDSVYAALDKSGGGSLEVVVSESGWPTQGGPGASAENARIYIYNLIQHVKNGSPRRPWKAIETYIFAMFDENERLDGSGMSYFGLFL PTTKQLKYGVNF | |||
| Full Sequence |
|---|
| Protein Sequence Length: 465 Download |
| MANFSSFHIL FFMFITSGVA AFATEDSCPS TVWQGTLTRK DDNNLGAGGF QLNPGASAQL 60 TASSEWSGHV WARTGCEFDS SGQGSCVIGD CGVDFTCAGS GGDSSVTMAE FIHGEKDYFD 120 VVDGTNVKLG VKPQGDNNYL ITFCPATTAE PIGVCFGQFG SNLPKPSDVV AMYKQYNIRR 180 MRMYGPNPEA LDALRGSNIE LILDVPNGYL KRLADSQTEA NTWVRDNVQK YNDVRFKYIS 240 VGNEVMPRGP GGAGTVLIQA MQKIDKALSE AGLSIPVSTT TNMGAFTDTY PPSRGRFSDE 300 YRNFLQPVIG FLVSKRSPLL VNIYTYFGFK NGNFSLDFAL FIPSNDEFKD PNNQHYYQNL 360 FDANLDSVYA ALDKSGGGSL EVVVSESGWP TQGGPGASAE NARIYIYNLI QHVKNGSPRR 420 PWKAIETYIF AMFDENERLD GSGMSYFGLF LPTTKQLKYG VNFN* 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd09215 | Thaumatin-like | 2.0e-24 | 22 | 138 | 123 | + the sweet-tasting protein, thaumatin, and thaumatin-like proteins involved in host defense. This family is represented by the sweet-tasting protein thaumatin from the African berry Thaumatococcus daniellii and thaumatin-like proteins (TLPs) involved in host defense and a wide range of developmental processes in fungi, plants, and animals. Plant TLPs are classified as pathogenesis-related (PR) protein family 5 (PR5), their expression is induced by environmental stresses such as pathogen/pest attack, drought and cold. TLPs included in this family are such proteins as zeamatin, found in high concentrations in cereal seeds; osmotin, a salt-induced protein in osmotically stressed plants; and PpAZ44, a propylene-induced TLP in abscission of young fruit. Several members of the plant TLP family have been reported as food allergens from fruits (i.e., cherry, Pru av 2; bell pepper, Cap a1; tomatoes, Lyc e NP24) and pollen allergens from conifers (i.e., mountain cedar, Jun a 3; Arizona cypress, Cup a3; Japanese cedar, Cry j3). Thaumatin and TLPs are three-domain, crescent-fold structures with either an electronegative, electropositive, or neutral cleft occurring between domains I and II. It has been proposed that the antifungal activity of plant PR5 proteins relies on the strong electronegative character of this cleft. Some TLPs hydrolyze the beta-1,3-glucans of the type commonly found in fungal walls. Most TLPs contain 16 conserved Cys residues. A deletion within the third domain (domain II) of the Triticum aestivum thaumatin-like xylanase inhibitor is observed, thus, only 10 conserved Cys residues are present within this smaller TLP and similar homologs. | ||
| smart00205 | THN | 8.0e-31 | 22 | 135 | 120 | + Thaumatin family. The thaumatin family gathers proteins related to plant pathogenesis. The thaumatin family includes very basic members with extracellular and vacuolar localization. Thaumatin itsel is a potent sweet-tasting protein. Several members of this family display significant in vitro activity of inhibiting hyphal growth or spore germination of various fungi probably by a membrane permeabilizing mechanism. | ||
| cd09218 | TLP-PA | 1.0e-34 | 27 | 135 | 114 | + allergenic/antifungal thaumatin-like proteins: plant and animal homologs. This subfamily is represented by the thaumatin-like proteins (TLPs), Cherry Allergen Pru Av 2 TLP, Peach PpAZ44 TLP (a propylene-induced TLP in abscission), the Caenorhabditis elegans thaumatin family member (thn-6), and other plant and animal homologs. TLPs are involved in host defense and a wide range of developmental processes in fungi, plants, and animals. Due to their inducible expression by environmental stresses such as pathogen/pest attack, drought and cold, plant TLPs are classified as the pathogenesis-related (PR) protein family 5 (PR5). Several members of the plant TLP family have been reported as food allergens from fruits (i.e., cherry, Pru av 2; bell pepper, Cap a1; tomatoes, Lyc e NP24) and pollen allergens from conifers (i.e., mountain cedar, Jun a 3; Arizona cypress, Cup a3; Japanese cedar, Cry j3). TLPs are three-domain, crescent-fold structures with either an electronegative, electropositive, or neutral cleft occurring between domains I and II. It has been proposed that the antifungal activity of plant PR5 proteins relies on the strong electronegative character of this cleft. Some TLPs hydrolyze the beta-1,3-glucans of the type commonly found in fungal walls. TLPs within this subfamily contain 16 conserved Cys residues. | ||
| pfam00314 | Thaumatin | 5.0e-36 | 28 | 146 | 124 | + Thaumatin family. | ||
| pfam00332 | Glyco_hydro_17 | 9.0e-126 | 152 | 463 | 317 | + Glycosyl hydrolases family 17. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAX76839.1 | 0 | 147 | 464 | 42 | 363 | beta-1,3-glucanase [Brassica rapa subsp. chinensis] |
| GenBank | ABC94639.1 | 0 | 147 | 464 | 29 | 346 | glucanase [Brassica juncea] |
| GenBank | ABO87659.1 | 0 | 147 | 464 | 29 | 351 | beta-1,3 glucanase [Brassica oleracea] |
| RefSeq | NP_191285.1 | 0 | 148 | 464 | 28 | 339 | BGL2 (BETA-1,3-GLUCANASE 2); cellulase/ glucan 1,3-beta-glucosidase/ hydrolase, hydrolyzing O-glycosyl compounds [Arabidopsis thaliana] |
| Swiss-Prot | P49236 | 0 | 147 | 464 | 22 | 342 | E13B_BRACM RecName: Full=Glucan endo-1,3-beta-glucosidase; AltName: Full=(1- |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3f55_D | 0 | 152 | 464 | 2 | 316 | A Chain A, Crystal Structure Of The Clp Gene Regulator Clgr From Corynebacterium Glutamicum |
| PDB | 3f55_C | 0 | 152 | 464 | 2 | 316 | A Chain A, Crystal Structure Of The Clp Gene Regulator Clgr From Corynebacterium Glutamicum |
| PDB | 3f55_B | 0 | 152 | 464 | 2 | 316 | A Chain A, Crystal Structure Of The Clp Gene Regulator Clgr From Corynebacterium Glutamicum |
| PDB | 3f55_A | 0 | 152 | 464 | 2 | 316 | A Chain A, Crystal Structure Of The Clp Gene Regulator Clgr From Corynebacterium Glutamicum |
| PDB | 3em5_D | 0 | 152 | 464 | 2 | 316 | A Chain A, Crystal Structure Of A Native Endo Beta-1,3-Glucanase (Hev B 2), A Major Allergen From Hevea Brasiliensis |
