| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra016262 |
| Family | CBM45 |
| Protein Properties | Length: 897 Molecular Weight: 100145 Isoelectric Point: 5.7922 |
| Chromosome | Chromosome/Scaffold: 07 Start: 18572951 End: 18577588 |
| Description | alpha-amylase-like 3 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CBM45 | 314 | 390 | 2.5e-25 |
| VHWGVCKNGNKKWEIPAEPYPEETSLFKNKALRTRLQRKDDGNGSSGLFSLDGELEGLCFVLKLNENTWLNNRGEDF | |||
| CBM45 | 127 | 210 | 4.5e-29 |
| LHWGVSYVGDTGSEWDQPPEDMRPPGSLAIKDYAIETPLEKLSEGDSFHEVTINLNLESSVAVLNFVLKDEETGAWYQHKGRDF | |||
| GH13 | 525 | 815 | 3.6e-38 |
| LQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYNLNSRYGTIDELKETVKRFHKVGIKVLGDAVLNHRCAHFKNSNGVWNLFGGRLNWDDRAVVADD PHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMREEVGYDGWRLDFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIV DWINATSGAAGAFDVTTKGILHAALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPAVF | |||
| Full Sequence |
|---|
| Protein Sequence Length: 897 Download |
| MSTVPIEPLL HLSPLRRTST IHRGPTTSSL NLRCHFTSKK LHSIGRSIAC GNSLGFRRRS 60 VAIRASSSDT AVVETTSQSD DVIIFKGNFP VERIDKAEGK IYVRLKQVKE NDWELSVGCS 120 LPGKWILHWG VSYVGDTGSE WDQPPEDMRP PGSLAIKDYA IETPLEKLSE GDSFHEVTIN 180 LNLESSVAVL NFVLKDEETG AWYQHKGRDF KVPLLDDVPD DGNLIGAKKG FGALGKLSNI 240 VVKPDEPGAD VQEKGKSSSG STKEGKGLEE FYEEMPISKH VAGDNSVSVT ARKCPETSKN 300 IVSIETDLPG DVTVHWGVCK NGNKKWEIPA EPYPEETSLF KNKALRTRLQ RKDDGNGSSG 360 LFSLDGELEG LCFVLKLNEN TWLNNRGEDF YVPFLTSAAL PVESGAAQVS GKTPKTNQEV 420 SDSGFTAEII TEIRNLAIDI SSHKNQKTNV KEVQENILQE IEKLAAEAYS IFRSTTPAFT 480 PESDLESEDE KPEIKISSGT GSGFEILCQG FNWESHKSGR WYQELQEKAD ELASLGFTVL 540 WLPPPTESVS PEGYMPKDLY NLNSRYGTID ELKETVKRFH KVGIKVLGDA VLNHRCAHFK 600 NSNGVWNLFG GRLNWDDRAV VADDPHFQGR GNKSSGDNFH AAPNIDHSQD FVRKDIKEWL 660 CWMREEVGYD GWRLDFVRGF WGGYVKDYMD ASKPYFAVGE YWDSLSYTYG EMDYNQDAHR 720 QRIVDWINAT SGAAGAFDVT TKGILHAALQ KCEYWRLSDP KGKPPGVVGW WPSRAVTFIE 780 NHDTGSTQGH WRFPGGKEMQ GYAYILTHPG TPAVFFDHIY SDYRSEIASL LSLRNRQKLH 840 CRSEVNIVKS ERDVYAAIID EKVAMKIGPG HYEPPSGSQN WSVAVEGRDY KVWETS* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PRK09441 | PRK09441 | 3.0e-48 | 505 | 823 | 396 | + cytoplasmic alpha-amylase; Reviewed | ||
| PLN00196 | PLN00196 | 9.0e-138 | 496 | 894 | 416 | + alpha-amylase; Provisional | ||
| cd11314 | AmyAc_arch_bac_plant_AmyA | 1.0e-163 | 506 | 845 | 343 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02361 | PLN02361 | 8.0e-173 | 502 | 894 | 399 | + alpha-amylase | ||
| PLN02784 | PLN02784 | 0 | 1 | 896 | 904 | + alpha-amylase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004556 | alpha-amylase activity |
| GO:0005509 | calcium ion binding |
| GO:0005975 | carbohydrate metabolic process |
| GO:0043169 | cation binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAG52558.1 | 2e-18 | 1 | 121 | 1 | 119 | AC010675_6 putative alpha-amylase; 60344-64829 [Arabidopsis thaliana] |
| GenBank | AAG52558.1 | 0 | 157 | 896 | 95 | 826 | AC010675_6 putative alpha-amylase; 60344-64829 [Arabidopsis thaliana] |
| GenBank | AAX33231.1 | 0 | 1 | 896 | 1 | 901 | plastid alpha-amylase [Malus x domestica] |
| RefSeq | NP_564977.1 | 0 | 1 | 896 | 1 | 887 | AMY3 (ALPHA-AMYLASE-LIKE 3); alpha-amylase [Arabidopsis thaliana] |
| RefSeq | XP_002520134.1 | 0 | 1 | 896 | 1 | 900 | alpha-amylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2qpu_C | 0 | 505 | 894 | 2 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 2qpu_B | 0 | 505 | 894 | 2 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 2qpu_A | 0 | 505 | 894 | 2 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 3bsg_A | 0 | 505 | 894 | 2 | 403 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
| PDB | 1rpk_A | 0 | 505 | 894 | 2 | 403 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1823 | EC-3.2.1.1 | α-amylase |
| starch degradation I | RXN-1825 | EC-3.2.1.1 | α-amylase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EG631183 | 911 | 1 | 897 | 0 |
| HO826981 | 407 | 491 | 897 | 0 |
| GR441435 | 293 | 449 | 741 | 0 |
| ES805448 | 328 | 462 | 789 | 0 |
| HO811991 | 299 | 599 | 897 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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