| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra016519 |
| Family | CE10 |
| Protein Properties | Length: 328 Molecular Weight: 36724.5 Isoelectric Point: 5.04 |
| Chromosome | Chromosome/Scaffold: 08 Start: 18857629 End: 18858612 |
| Description | alpha/beta-Hydrolases superfamily protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE10 | 32 | 324 | 0 |
| VPPSLTPQNGVVSKDAIYSPEKNLSLRIYLPHQTLETSEANKKKKKLPLLIYFHGGAFIMETAFSPAYNTFLTSTVSAAGCIAFSVDYRRAPVAEHPIPI PYEDSWDAVKWIFTHVAGSGPEDLVNDHADFRRVFVAGDSAGANIAHHMAIRAGEENGSIKFSGMTLFHPFFFSKAILEEQEDGVRRYMEGIWEIVCPNS KKGVEDPWINVVGSDLSGLECGRVLVMVAGKDLLAREGRVYAEKLEECGWGGRVEVVETEDEDHVFHIRNPDSDNARLLVQRFAEFLRQVCSD | |||
| Full Sequence |
|---|
| Protein Sequence Length: 328 Download |
| MDSDQELAYD YSPRFRVYKN GQIQRLVAET FVPPSLTPQN GVVSKDAIYS PEKNLSLRIY 60 LPHQTLETSE ANKKKKKLPL LIYFHGGAFI METAFSPAYN TFLTSTVSAA GCIAFSVDYR 120 RAPVAEHPIP IPYEDSWDAV KWIFTHVAGS GPEDLVNDHA DFRRVFVAGD SAGANIAHHM 180 AIRAGEENGS IKFSGMTLFH PFFFSKAILE EQEDGVRRYM EGIWEIVCPN SKKGVEDPWI 240 NVVGSDLSGL ECGRVLVMVA GKDLLAREGR VYAEKLEECG WGGRVEVVET EDEDHVFHIR 300 NPDSDNARLL VQRFAEFLRQ VCSDDKE* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00312 | Esterase_lipase | 0.0005 | 18 | 210 | 201 | + Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate. | ||
| PRK10162 | PRK10162 | 7.0e-6 | 81 | 248 | 177 | + acetyl esterase; Provisional | ||
| COG2272 | PnbA | 2.0e-7 | 28 | 177 | 161 | + Carboxylesterase type B [Lipid metabolism] | ||
| COG0657 | Aes | 2.0e-27 | 21 | 319 | 309 | + Esterase/lipase [Lipid metabolism] | ||
| pfam07859 | Abhydrolase_3 | 2.0e-55 | 81 | 280 | 207 | + alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAO41964.1 | 0 | 7 | 320 | 1 | 308 | putative esterase [Arabidopsis thaliana] |
| GenBank | AAT70485.1 | 0 | 15 | 320 | 2 | 301 | At2g03550 [Arabidopsis thaliana] |
| RefSeq | NP_173353.1 | 0 | 4 | 320 | 2 | 315 | hydrolase [Arabidopsis thaliana] |
| RefSeq | NP_178453.1 | 0 | 4 | 320 | 2 | 312 | hydrolase [Arabidopsis thaliana] |
| RefSeq | NP_190439.1 | 0 | 4 | 319 | 2 | 326 | ATCXE13 (ARABIDOPSIS THALIANA CARBOXYESTERASE 13); hydrolase [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ed1_F | 5e-29 | 3 | 318 | 24 | 347 | A Chain A, Pectin Methylesterase From Carrot |
| PDB | 3ed1_E | 5e-29 | 3 | 318 | 24 | 347 | A Chain A, Pectin Methylesterase From Carrot |
| PDB | 3ed1_D | 5e-29 | 3 | 318 | 24 | 347 | A Chain A, Pectin Methylesterase From Carrot |
| PDB | 3ed1_C | 5e-29 | 3 | 318 | 24 | 347 | A Chain A, Pectin Methylesterase From Carrot |
| PDB | 3ed1_B | 5e-29 | 3 | 318 | 24 | 347 | A Chain A, Pectin Methylesterase From Carrot |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| formononetin biosynthesis | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| formononetin biosynthesis | RXN-3625 | - | 2,7-dihydroxy-4'-methoxyisoflavanone dehydratase |
| isoflavonoid biosynthesis I | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-3303 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-5502 | - | 2,7,5-trihydroxy-4'-methoxyisoflavanone dehydratase |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| ES909331 | 297 | 5 | 301 | 0 |
| EX133191 | 301 | 7 | 302 | 0 |
| DK473239 | 222 | 1 | 221 | 0 |
| DK532857 | 219 | 108 | 326 | 0 |
| DK473239 | 79 | 199 | 277 | 6e-27 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
