| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra017241 |
| Family | GH13 |
| Protein Properties | Length: 2271 Molecular Weight: 257031 Isoelectric Point: 6.0857 |
| Chromosome | Chromosome/Scaffold: 04 Start: 15804449 End: 15818281 |
| Description | pleiotropic drug resistance 11 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 1772 | 2092 | 3.2e-28 |
| LPRIKKLGYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRFGTPDDLKSLIDKAHELGLVVLMDIVHSHASKNTLDGLNMFDGTDGQYFHSGERGYHWMWD SRLFNYGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVEFTGNYSEYFGYSTDVDAVVYLMLVNDMIHGLYPEAIVVGEDVSGMPAFCIP VQDGGVGFDYRLHMAVADKWIELLKKRDEDWQVGDIVFTLTNRRWGEKCVVYAESHDQALVGDKTIAFWLMDKDMYDFMAVDGQATPRVDRGIALHKMIR LVTMGLGGEGYLNFMGNEFGH | |||
| Full Sequence |
|---|
| Protein Sequence Length: 2271 Download |
| MLGRDEDLHV RTMSGRGSLG STSHRSLPGA SKSFRDVFAP PAADEVFGSS KRREDDDVEL 60 RWAAIERLPT FDRLRKGMLP QEATVNGKGK LEEVDLTNLA PKEKKHLMEM IFKFVEEDNE 120 KFLRRLRERT DRVGIEVPKI EVRYENISVE GDVRSASRAL PTLFNVTLNT LESILGMCHL 180 LPSKKRKIQI LKNISGIVKP SRMTLLLGPP SSGKTTLLQV LAGKLDDTLQ MSGKITYCGH 240 EFREFVPQKT CAYISQHDLH FGEMTVRETL DFSGRCLGVG TRYQLLTELS RREREAGIKP 300 DPEIDAFMKS IAISGQETSL VTDYVLKILG LDICADILVG DVMRRGVSGG QRKRLTTGEM 360 LVGPATALFM DEISTGLDSS TTFQICKFMR QLVHISDVTM IISLLQPAPE TFELFDDIIL 420 LSEGHIVYQG PRDNVLEFFE YMGFQCPERK GVADFLQEVT SKKDQEQYWN RREQPYSYVS 480 VNDFSTGFKS FHTGQQLASE IRTPYDKSKT HPAALVTQKY GISNWELFKA CFDREWLLMK 540 RNSFIYVFKT VQITIMSLIA MTVYLRTEMH VGTVQDGQKF YGALFFSLIN VMFNGMAELA 600 FTVMRLPVFY KQRDFLFYPA WAFALPAWLL KIPLSLIESG IWIVLTYYTI GFAPAASRFF 660 RQLLAYFCVN QMALSLFRFL GAVGRTEVIS NSVGTFTLLI VFTLGGFIIA KDDIPPWMTW 720 AYYISPMMYG QTAIVMNEFL DERWGAPNTD TRIDAKTVGE VLLKSRGFFT EPYWFWICIV 780 ALLGFSLLFN LFYIIALMYL NPLGDSKATV VEEGKDKHKG NSRGPDSIVE LSNRSSNGPK 840 RGMVLPFQPL SLAFQNVNYY VDMPAEMKAQ GVEGDRLQLL RDVGGAFRPG VLTALVGVSG 900 AGKTTLMDVL AGRKTGGYVE GSISISGYPK NQSTFARVSG YCEQNDIHSP HVTVYESLIY 960 SAWLRLSVDI DAKTREMFVE EVMELVELKP LRNSIVGLPG VDGLSTEQRK RLTIAVELVA 1020 NPSIIFMDEP TSGLDARAAA IVMRTVRNTV DTGRTVVCTI HQPSIDIFES FDELLLMKRG 1080 GQVIYAGSLG HHSQKLVEYF EAVEGVPKIK DGYNPATWML DVTTPSMESQ MSLDFAQLFA 1140 NSSLYRRNQE LIKQLSTPPP GSNDLYFPTK YSQPFWTQTK ACFWKQYWSN WRYPQYNAIR 1200 FLMTIVIGVM FGLIFWQTGT KIEKEQDLNN FFGAMYAAIL FLGATNAATV QPAIAIERTV 1260 FYREKAAGMY SAIPYAISQV AVEIMYNTIQ TAVYTLILYS MIGYDWTVAK FLWFYYYMLT 1320 SFIYFTLYGM MLVALTPNYQ IAGICMSFFL SLWNLFSGFL IPRPQIPIWW RWYYWATPVA 1380 WTLYGIITSQ VGDQDSIVQI AGVGNMSLKT LMKDGFGFEH DFLPVVAAVH IGWILLFVFV 1440 FAYGIKFLNF QRSFSLSHSL TLSLLYLAMV YTMSGVRFAH LPSIKSSSLP TFNDDRRGNA 1500 VSFSLRKDTR SSGKIFGRKP SSDSESSSLA TTPSEKLGGH QSDSSSAVTG HVEPLETVSQ 1560 DTTQVLDDVH AQRIETEGQD TSGSMSESFV QEVGQRRIPP PGDGKKIYDI DPMLKSHNGH 1620 LDYRYGQYIK LREQIDKNEG GLEAFSRGYE IFGFTRSATG ITYREWAPGA KAASLIGDFN 1680 NWNSKVDVMS RNEFGVWEIF LPNNADGSPA IPHGSRVKIR MDTPTGIKDS IPAWIKYSVQ 1740 APGEIPYNGV YYDPAEEEPM INTYANFRDD VLPRIKKLGY NAVQIMAIQE HSYYASFGYH 1800 VTNFFAPSSR FGTPDDLKSL IDKAHELGLV VLMDIVHSHA SKNTLDGLNM FDGTDGQYFH 1860 SGERGYHWMW DSRLFNYGSW EVLRYLLSNA RWWLEEYKFD GFRFDGVTSM MYTHHGLQVE 1920 FTGNYSEYFG YSTDVDAVVY LMLVNDMIHG LYPEAIVVGE DVSGMPAFCI PVQDGGVGFD 1980 YRLHMAVADK WIELLKKRDE DWQVGDIVFT LTNRRWGEKC VVYAESHDQA LVGDKTIAFW 2040 LMDKDMYDFM AVDGQATPRV DRGIALHKMI RLVTMGLGGE GYLNFMGNEF GHPEWIDFPR 2100 TDQHLPDGRV IPGNNASYDK CRRRFDLGDA EYLRYHGLQE FDRAMQHLEE KYGFMTSEHL 2160 YISRKDEGDR VVVFERGSLV FVFNFHWTNS YSDYRIGCSV PGKYKIVLDS DGSLFGGFGR 2220 LDDSAEFFTN DGRYDDRPCS LMVYAPCRTA VVYAAVDGDE DSISLVPEDV * 2280 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02960 | PLN02960 | 3.0e-162 | 1709 | 2253 | 577 | + alpha-amylase | ||
| PLN03140 | PLN03140 | 0 | 12 | 1451 | 1472 | + ABC transporter G family member; Provisional | ||
| PLN02447 | PLN02447 | 0 | 1530 | 2267 | 772 | + 1,4-alpha-glucan-branching enzyme | ||
| TIGR00956 | 3a01205 | 0 | 116 | 1395 | 1346 | + Pleiotropic Drug Resistance (PDR) Family protein. [Transport and binding proteins, Other]. | ||
| cd11321 | AmyAc_bac_euk_BE | 0 | 1755 | 2143 | 390 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005524 | ATP binding |
| GO:0005975 | carbohydrate metabolic process |
| GO:0016020 | membrane |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAD24623.1 | 0 | 1 | 1452 | 1 | 1449 | putative ABC transporter [Arabidopsis thaliana] |
| GenBank | AAF98206.1 | 0 | 1 | 1452 | 4 | 1434 | AC007152_2 Putative ABC transporter [Arabidopsis thaliana] |
| GenBank | AAG50592.1 | 0 | 1 | 1452 | 4 | 1433 | AC083891_6 ABC transporter, putative [Arabidopsis thaliana] |
| RefSeq | NP_176867.2 | 0 | 1 | 1452 | 4 | 1453 | PDR11 (PLEIOTROPIC DRUG RESISTANCE 11); ATPase, coupled to transmembrane movement of substances [Arabidopsis thaliana] |
| RefSeq | NP_181179.2 | 0 | 1 | 1452 | 1 | 1452 | PDR6; ATPase, coupled to transmembrane movement of substances [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3amk_A | 0 | 1603 | 2263 | 9 | 700 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3vu2_B | 0 | 1603 | 2263 | 9 | 700 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3vu2_A | 0 | 1603 | 2263 | 9 | 700 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3aml_A | 0 | 1607 | 2263 | 13 | 700 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 1m7x_D | 6.02558e-44 | 1647 | 2217 | 9 | 577 | A Chain A, The X-Ray Crystallographic Structure Of Branching Enzyme |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch biosynthesis | RXN-7710 | EC-2.4.1.18 | 1,4-α-glucan branching enzyme |
