| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra017301 |
| Family | GT8 |
| Protein Properties | Length: 495 Molecular Weight: 56515.1 Isoelectric Point: 8.2947 |
| Chromosome | Chromosome/Scaffold: 04 Start: 15483688 End: 15487282 |
| Description | Nucleotide-diphospho-sugar transferases superfamily protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT8 | 73 | 293 | 3.9e-28 |
| DYEFYVATRVLIRSLRDLNVEADLVVIASLDVPLRWVETLEEEDGAKVVRVENVDNPYRGQTNFNSRFKLTLNKLYAWALSDYDRVVMLDADNLFLKKTD ELFQCGRFCAVFINPCIFHTGLFVLQPSLEVFKDMLHELQVGRENRDGADQGFLVSYFSDLLDQPLFRPPSNGSVLSGHLRLPLGYQMDASYFYLKLRWN IPCGPNSVITFPGAVWLKPWY | |||
| Full Sequence |
|---|
| Protein Sequence Length: 495 Download |
| MDMHRGLVLL CCVLSLLVIE TTAYRERQLL QLHETTVDTT NTAVAVRDRG LKTRRPEHKN 60 AYATMMYMGT PRDYEFYVAT RVLIRSLRDL NVEADLVVIA SLDVPLRWVE TLEEEDGAKV 120 VRVENVDNPY RGQTNFNSRF KLTLNKLYAW ALSDYDRVVM LDADNLFLKK TDELFQCGRF 180 CAVFINPCIF HTGLFVLQPS LEVFKDMLHE LQVGRENRDG ADQGFLVSYF SDLLDQPLFR 240 PPSNGSVLSG HLRLPLGYQM DASYFYLKLR WNIPCGPNSV ITFPGAVWLK PWYWWSWPVL 300 PLGFSWHEQR RTTIGYSAEM PLVVIQTMFY LGIIVVTRLA RPNITKLCYR RSDRNLTTIQ 360 AGFKFIALLS VVAAYVFPFF TIPHTIHPLI GWSLYLMASF ALSSIPINTL LLPTLPVLTP 420 WIGILGTLLV MAFPWYPDGV VRALSVFGYA FCCAPFVWVA FLKITSHLQV LIEKEVLFPR 480 LGDSGITSGF SKLY* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG5597 | COG5597 | 4.0e-6 | 51 | 251 | 216 | + Alpha-N-acetylglucosamine transferase [Cell envelope biogenesis, outer membrane] | ||
| PLN00176 | PLN00176 | 2.0e-7 | 83 | 232 | 187 | + galactinol synthase | ||
| cd06914 | GT8_GNT1 | 2.0e-8 | 62 | 292 | 255 | + GNT1 is a fungal enzyme that belongs to the GT 8 family. N-acetylglucosaminyltransferase is a fungal enzyme that catalyzes the addition of N-acetyl-D-glucosamine to mannotetraose side chains by an alpha 1-2 linkage during the synthesis of mannan. The N-acetyl-D-glucosamine moiety in mannan plays a role in the attachment of mannan to asparagine residues in proteins. The mannotetraose and its N-acetyl-D-glucosamine derivative side chains of mannan are the principle immunochemical determinants on the cell surface. N-acetylglucosaminyltransferase is a member of glycosyltransferase family 8, which are, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed, retaining glycosyltransferases. | ||
| pfam01501 | Glyco_transf_8 | 5.0e-12 | 73 | 296 | 262 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd02537 | GT8_Glycogenin | 1.0e-67 | 60 | 311 | 262 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_193393.4 | 0 | 1 | 494 | 1 | 494 | transferase, transferring glycosyl groups / transferase, transferring hexosyl groups [Arabidopsis thaliana] |
| RefSeq | NP_565817.2 | 0 | 1 | 494 | 1 | 497 | glycogenin glucosyltransferase (glycogenin)-related [Arabidopsis thaliana] |
| RefSeq | NP_973607.1 | 0 | 113 | 494 | 8 | 389 | glycogenin glucosyltransferase (glycogenin)-related [Arabidopsis thaliana] |
| RefSeq | XP_002269578.1 | 0 | 57 | 484 | 36 | 463 | PREDICTED: hypothetical protein isoform 1 [Vitis vinifera] |
| RefSeq | XP_002528922.1 | 0 | 55 | 494 | 44 | 483 | glucosyltransferase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3u2x_B | 0.0000000000002 | 82 | 231 | 22 | 173 | A Chain A, Crystal Structure Of A Native Endo Beta-1,3-Glucanase (Hev B 2), A Major Allergen From Hevea Brasiliensis |
| PDB | 3u2x_A | 0.0000000000002 | 82 | 231 | 22 | 173 | A Chain A, Crystal Structure Of A Native Endo Beta-1,3-Glucanase (Hev B 2), A Major Allergen From Hevea Brasiliensis |
| PDB | 3u2v_B | 0.0000000000002 | 82 | 231 | 22 | 173 | A Chain A, Crystal Structure Of A Native Endo Beta-1,3-Glucanase (Hev B 2), A Major Allergen From Hevea Brasiliensis |
| PDB | 3u2v_A | 0.0000000000002 | 82 | 231 | 22 | 173 | A Chain A, Crystal Structure Of A Native Endo Beta-1,3-Glucanase (Hev B 2), A Major Allergen From Hevea Brasiliensis |
| PDB | 3u2u_B | 0.0000000000002 | 82 | 231 | 22 | 173 | A Chain A, Crystal Structure Of A Native Endo Beta-1,3-Glucanase (Hev B 2), A Major Allergen From Hevea Brasiliensis |
