| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra018809 |
| Family | CE10 |
| Protein Properties | Length: 317 Molecular Weight: 35108.7 Isoelectric Point: 5.9513 |
| Chromosome | Chromosome/Scaffold: 06 Start: 1820313 End: 1821263 |
| Description | alpha/beta-Hydrolases superfamily protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE10 | 34 | 311 | 0 |
| DPQNDVVTKDVVYSPEHNLSVRLFLPHKSTKLAAGKKLPLLIYFHGGAYIMESPFSPVYHNYLAEVVKTSNCLAASVQYRRAPEHPIPAAYEDSWAAIQW IFSHSDGFGEEVWINELADFGRVFVAGANISHHMAIKAGKEKLHPRIKGVAMVHPGFWGKDPVDEKDVQDERIRSQVAEVWELASPNSVDGVDDPWLNVV GSGSDISGLGCERVLVAVAGKDVFGRQGLAYAAKLEKSGWRGELEVVEDEEEGHCFHIHNANSENALKLMKTFVEFMS | |||
| Full Sequence |
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| Protein Sequence Length: 317 Download |
| MEPHRTSHLP PIRIHKDGRV ERLSGNDVIP TSLDPQNDVV TKDVVYSPEH NLSVRLFLPH 60 KSTKLAAGKK LPLLIYFHGG AYIMESPFSP VYHNYLAEVV KTSNCLAASV QYRRAPEHPI 120 PAAYEDSWAA IQWIFSHSDG FGEEVWINEL ADFGRVFVAG ANISHHMAIK AGKEKLHPRI 180 KGVAMVHPGF WGKDPVDEKD VQDERIRSQV AEVWELASPN SVDGVDDPWL NVVGSGSDIS 240 GLGCERVLVA VAGKDVFGRQ GLAYAAKLEK SGWRGELEVV EDEEEGHCFH IHNANSENAL 300 KLMKTFVEFM SSTVEE* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00135 | COesterase | 0.0004 | 45 | 113 | 71 | + Carboxylesterase family. | ||
| cd00312 | Esterase_lipase | 6.0e-5 | 52 | 188 | 153 | + Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate. | ||
| COG2272 | PnbA | 5.0e-5 | 30 | 113 | 84 | + Carboxylesterase type B [Lipid metabolism] | ||
| COG0657 | Aes | 1.0e-23 | 54 | 272 | 228 | + Esterase/lipase [Lipid metabolism] | ||
| pfam07859 | Abhydrolase_3 | 4.0e-50 | 74 | 291 | 228 | + alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM61628.1 | 0 | 10 | 311 | 67 | 373 | putative esterase [Arabidopsis thaliana] |
| GenBank | AAT68324.1 | 0 | 1 | 312 | 1 | 315 | hypothetical protein At1g49640 [Arabidopsis thaliana] |
| RefSeq | NP_175387.1 | 0 | 1 | 312 | 1 | 315 | hydrolase [Arabidopsis thaliana] |
| RefSeq | NP_175389.1 | 0 | 6 | 310 | 7 | 317 | AtCXE5 (Arabidopsis thaliana carboxyesterase 5); carboxylesterase |
| RefSeq | NP_564550.1 | 0 | 10 | 311 | 67 | 373 | cell death associated protein-related [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2o7v_A | 8e-38 | 11 | 272 | 23 | 293 | A Chain A, Crystal Structure Of The Glycogen Synthase From A. Tumefaciens In Complex With Adp |
| PDB | 2o7r_A | 8e-38 | 11 | 272 | 23 | 293 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 2zsi_A | 4e-26 | 29 | 308 | 60 | 349 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 2zsh_A | 4e-26 | 29 | 308 | 60 | 349 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 3ed1_F | 3e-24 | 63 | 314 | 104 | 351 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| formononetin biosynthesis | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| formononetin biosynthesis | RXN-3625 | - | 2,7-dihydroxy-4'-methoxyisoflavanone dehydratase |
| isoflavonoid biosynthesis I | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-3303 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-5502 | - | 2,7,5-trihydroxy-4'-methoxyisoflavanone dehydratase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EV067951 | 208 | 1 | 204 | 0 |
| DK458597 | 269 | 1 | 263 | 0 |
| FG570445 | 197 | 1 | 193 | 0 |
| CD817022 | 211 | 1 | 207 | 0 |
| FG570445 | 29 | 190 | 218 | 0.000001 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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