| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra019043 |
| Family | GT20 |
| Protein Properties | Length: 787 Molecular Weight: 88605.1 Isoelectric Point: 6.5435 |
| Chromosome | Chromosome/Scaffold: 03 Start: 26584328 End: 26588440 |
| Description | trehalose-6-phosphatase synthase S4 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 4 | 469 | 0 |
| PRLLVVSMSLPVTAKRTGEEAWSFTMSPGGLVSALLGLKEFETKWIGWPGVDVHDAVGKKALSIALAEKGCIPVFLEEVCDQYYNGYCNNILWPIFHYLG TPPEYRNDGTITYQSQYDAYKKANRIFFDVVNEHYQEGDVVWCHDYHVMLLPQYLKEYNSKMKVGWFLHTPFPSSEMYKTLPSRSELLRSVLTADLVGFH TYDFARHFVNACMCILGTEATSEGVVDQGKFTSVEVFPIGIEPERFINTSKLSEVTEYMKKFKSDFGGRKLILGVDRLDTIKGIPHKYQAFEKFLEENED WRGKVLLLQIAVPTRNGIGEYQKIKDQCHGVVGRINGRFGSISSVPIIHLDCSIHFHQLCALYAITDVLLVTSLRDGMNLVSSEFVACQKGEKGVLVLSE FAGAGQSLGAGALLVNPWNIKEVSNAIGEALIMSPEEKEKKHQINFQYVKTHSTQQWADDFMNKLN | |||
| Full Sequence |
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| Protein Sequence Length: 787 Download |
| MVRPRLLVVS MSLPVTAKRT GEEAWSFTMS PGGLVSALLG LKEFETKWIG WPGVDVHDAV 60 GKKALSIALA EKGCIPVFLE EVCDQYYNGY CNNILWPIFH YLGTPPEYRN DGTITYQSQY 120 DAYKKANRIF FDVVNEHYQE GDVVWCHDYH VMLLPQYLKE YNSKMKVGWF LHTPFPSSEM 180 YKTLPSRSEL LRSVLTADLV GFHTYDFARH FVNACMCILG TEATSEGVVD QGKFTSVEVF 240 PIGIEPERFI NTSKLSEVTE YMKKFKSDFG GRKLILGVDR LDTIKGIPHK YQAFEKFLEE 300 NEDWRGKVLL LQIAVPTRNG IGEYQKIKDQ CHGVVGRING RFGSISSVPI IHLDCSIHFH 360 QLCALYAITD VLLVTSLRDG MNLVSSEFVA CQKGEKGVLV LSEFAGAGQS LGAGALLVNP 420 WNIKEVSNAI GEALIMSPEE KEKKHQINFQ YVKTHSTQQW ADDFMNKLNE VTSKAELETG 480 KAPHELPQHD VVQQYSKSKN RLLILGFYGT LTKPNKNQER KDEGMNLELH PQLKERLKGL 540 CSDPKTTVVV LSRSEKSVLD QNFGEHDMWL AAENGMFLRH TAGEWVTKMP ENMNLEWIDG 600 VKQVFKYFTE RTPGSYFQAS ETSVVWNYQY ADAEFGRAQA KDMLQHLWAG PTSNASVDVV 660 QGRYSVEVHS VGVTKGSAME RILGEIVLQN KSITTPIDYV LCIGNFLGKD EDVYTFFEPE 720 LTKKTMSSPY YGSGPLKKVS STLVDVKGDN YFSVAIGNTH TKASYFLDSS DDVVQLIHKL 780 CTHNNP* 840 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03063 | PLN03063 | 0 | 1 | 785 | 785 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03064 | PLN03064 | 0 | 3 | 786 | 841 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PRK14501 | PRK14501 | 0 | 5 | 780 | 785 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| TIGR02400 | trehalose_OtsA | 0 | 5 | 470 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| pfam00982 | Glyco_transf_20 | 0 | 4 | 470 | 475 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_173142.1 | 0 | 3 | 782 | 10 | 772 | ATTPS2; alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | NP_177979.1 | 0 | 3 | 721 | 91 | 812 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | NP_177979.1 | 0.0005 | 737 | 781 | 892 | 936 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | NP_194485.1 | 0 | 1 | 785 | 1 | 794 | ATTPS4; alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| Swiss-Prot | Q9FZ57 | 0 | 3 | 782 | 11 | 773 | TPS2_ARATH RecName: Full=Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 2; AltName: Full=Trehalose-6-phosphate synthase 2; Short=AtTPS2 |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1gz5_D | 0 | 30 | 474 | 19 | 456 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_C | 0 | 30 | 474 | 19 | 456 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_B | 0 | 30 | 474 | 19 | 456 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_A | 0 | 30 | 474 | 19 | 456 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 2wtx_D | 0 | 30 | 475 | 20 | 458 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EG462462 | 243 | 1 | 243 | 0 |
| ES813375 | 360 | 365 | 721 | 0 |
| DW069910 | 288 | 90 | 377 | 0 |
| CV711307 | 293 | 3 | 294 | 0 |
| EG462462 | 25 | 232 | 256 | 0.007 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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