| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra019558 |
| Family | CE10 |
| Protein Properties | Length: 324 Molecular Weight: 35520.4 Isoelectric Point: 5.0031 |
| Chromosome | Chromosome/Scaffold: 06 Start: 12225264 End: 12226235 |
| Description | alpha/beta-Hydrolases superfamily protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE10 | 12 | 320 | 0 |
| LLKIYKSGRIERLMGETAVPPSLTPENGVISKDVVYSPDDNLSVRVYLPEKAADTDEKLPLLVYFHGGGFIIETAFSPTYHNFLTAAVSASDCIAVSVDY RRAPEHPLPISFDDSWTSLKWVFTHIAGSGPESWLNKHADFGKVFLAGDSAGANIAHHMAMRAAKEKLSPELSDSGIFGIILVHPYFWSKAPVDDKETTD AAVRSKIEAIWMMASPNNKDGVDDPWLNVVQSDSVDISGLGCGKVLVLVAEKDALVRQGWGYKAKLEKNGWKGKVELVESEGEDHVFHLTRPDCENALEA MKKFAGFIK | |||
| Full Sequence |
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| Protein Sequence Length: 324 Download |
| MDSEIAFDYS PLLKIYKSGR IERLMGETAV PPSLTPENGV ISKDVVYSPD DNLSVRVYLP 60 EKAADTDEKL PLLVYFHGGG FIIETAFSPT YHNFLTAAVS ASDCIAVSVD YRRAPEHPLP 120 ISFDDSWTSL KWVFTHIAGS GPESWLNKHA DFGKVFLAGD SAGANIAHHM AMRAAKEKLS 180 PELSDSGIFG IILVHPYFWS KAPVDDKETT DAAVRSKIEA IWMMASPNNK DGVDDPWLNV 240 VQSDSVDISG LGCGKVLVLV AEKDALVRQG WGYKAKLEKN GWKGKVELVE SEGEDHVFHL 300 TRPDCENALE AMKKFAGFIK GEM* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00312 | Esterase_lipase | 5.0e-8 | 51 | 176 | 136 | + Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate. | ||
| pfam00135 | COesterase | 2.0e-10 | 51 | 179 | 148 | + Carboxylesterase family. | ||
| COG2272 | PnbA | 1.0e-12 | 51 | 176 | 140 | + Carboxylesterase type B [Lipid metabolism] | ||
| COG0657 | Aes | 4.0e-31 | 11 | 320 | 319 | + Esterase/lipase [Lipid metabolism] | ||
| pfam07859 | Abhydrolase_3 | 5.0e-61 | 73 | 300 | 233 | + alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAL57633.1 | 0 | 1 | 321 | 1 | 322 | AT3g48690/T8P19_200 [Arabidopsis thaliana] |
| DDBJ | BAE98942.1 | 0 | 1 | 321 | 1 | 322 | hypothetical protein [Arabidopsis thaliana] |
| RefSeq | NP_178453.1 | 0 | 1 | 319 | 1 | 310 | hydrolase [Arabidopsis thaliana] |
| RefSeq | NP_190438.1 | 0 | 1 | 321 | 1 | 322 | CXE12; carboxylesterase [Arabidopsis thaliana] |
| RefSeq | NP_190439.1 | 0 | 1 | 322 | 1 | 328 | ATCXE13 (ARABIDOPSIS THALIANA CARBOXYESTERASE 13); hydrolase [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2o7v_A | 6e-35 | 28 | 281 | 41 | 293 | A Chain A, Polygalacturonase From Erwinia Carotovora Ssp. Carotovora |
| PDB | 2o7r_A | 6e-35 | 28 | 281 | 41 | 293 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ed1_F | 2e-32 | 19 | 323 | 38 | 351 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ed1_E | 2e-32 | 19 | 323 | 38 | 351 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ed1_D | 2e-32 | 19 | 323 | 38 | 351 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| formononetin biosynthesis | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| formononetin biosynthesis | RXN-3625 | - | 2,7-dihydroxy-4'-methoxyisoflavanone dehydratase |
| isoflavonoid biosynthesis I | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-3303 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-5502 | - | 2,7,5-trihydroxy-4'-methoxyisoflavanone dehydratase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EX019947 | 281 | 1 | 281 | 0 |
| EX109246 | 276 | 1 | 276 | 0 |
| EX047810 | 270 | 24 | 293 | 0 |
| FY427215 | 250 | 75 | 324 | 0 |
| GR443701 | 249 | 2 | 250 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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