| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra019749 |
| Family | GH32 |
| Protein Properties | Length: 663 Molecular Weight: 73325.5 Isoelectric Point: 6.2117 |
| Chromosome | Chromosome/Scaffold: 06 Start: 4626655 End: 4630693 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 123 | 443 | 0 |
| HFQPGQNWMNDPNGPLFYKGWYHFFYQYNPNAAVWGDIVWGHAVSKDLIHWVHLPLAMVADQWYDANGVWTGSATFLEDGSIVMLYTGSTDKSVQVQNLA YPEDLNDPLLLKWVKFSGNPVLVPPPGILPKDFRDPTTAWKTSAGKWRITIGSKINRTGISLVYDTTDFKTYEKLDTLLHKVPNTGMWECVDFYPVSKTL VKGLDTSVNGPDVKHIVKASMDDTRIDHYAIGTYFDSNGTWIPDDPTIDVGISTSLRYDYGKFYASKTFYDQNKGRRILWGWIGESDSESADVQKGWSSL QGIPRTVVLDTKTGKNLVQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 663 Download |
| MASSDALLPI SAREEDPLLS DGSRSDPNAE THGRRRPVKG LLAVTFGLFF IAFYVALIAT 60 HDGSRSNDVK FESDGTATTA SRARLAGVSE KSNDQLWKLS GDRNTVAFSW NNSMLSWQRT 120 GFHFQPGQNW MNDPNGPLFY KGWYHFFYQY NPNAAVWGDI VWGHAVSKDL IHWVHLPLAM 180 VADQWYDANG VWTGSATFLE DGSIVMLYTG STDKSVQVQN LAYPEDLNDP LLLKWVKFSG 240 NPVLVPPPGI LPKDFRDPTT AWKTSAGKWR ITIGSKINRT GISLVYDTTD FKTYEKLDTL 300 LHKVPNTGMW ECVDFYPVSK TLVKGLDTSV NGPDVKHIVK ASMDDTRIDH YAIGTYFDSN 360 GTWIPDDPTI DVGISTSLRY DYGKFYASKT FYDQNKGRRI LWGWIGESDS ESADVQKGWS 420 SLQGIPRTVV LDTKTGKNLV QWPVDEVKSL RLSSKKFDME VGPGSFVNID VGSAAQLDIE 480 AEFEIKKESL DKILGDASAA AEAEEFSCQK SGGSTVRGAL GPFGFSVLAH ESLSEQTPVY 540 FYVAKGKDSK LKTFFCTDSS RSSFANDVVK PVYGSSVPVL EGEKLTMRIL VDHSIVEAFG 600 QGGRTCTTSR VYPTKAIYGA AKLFLFNNAI DATITASFNV WQMNSAFIHP YSEEAVRALS 660 RT* 720 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 2.0e-56 | 119 | 613 | 513 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 2.0e-67 | 119 | 614 | 508 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 8.0e-94 | 129 | 446 | 324 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 1.0e-149 | 123 | 443 | 329 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 0 | 123 | 603 | 491 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAG36942.1 | 0 | 1 | 662 | 1 | 663 | AF274298_1 acid invertase [Brassica oleracea] |
| GenBank | AAG36943.1 | 0 | 1 | 662 | 1 | 662 | AF274299_1 acid invertase [Brassica oleracea] |
| EMBL | CAA66330.1 | 0 | 5 | 662 | 1 | 660 | beta-fructosidase [Arabidopsis thaliana] |
| RefSeq | NP_563901.1 | 0 | 1 | 662 | 1 | 664 | ATBETAFRUCT4; beta-fructofuranosidase/ hydrolase, hydrolyzing O-glycosyl compounds [Arabidopsis thaliana] |
| RefSeq | NP_564798.1 | 0 | 1 | 662 | 1 | 648 | beta-fructosidase (BFRUCT3) / beta-fructofuranosidase / invertase, vacuolar [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 104 | 651 | 4 | 544 | A Chain A, Pectin Methylesterase Pema From Erwinia Chrysanthemi |
| PDB | 3ugh_A | 0 | 104 | 651 | 4 | 544 | A Chain A, Pectin Methylesterase Pema From Erwinia Chrysanthemi |
| PDB | 3ugg_B | 0 | 104 | 651 | 4 | 544 | A Chain A, Pectin Methylesterase Pema From Erwinia Chrysanthemi |
| PDB | 3ugg_A | 0 | 104 | 651 | 4 | 544 | A Chain A, Pectin Methylesterase Pema From Erwinia Chrysanthemi |
| PDB | 3ugf_B | 0 | 104 | 651 | 4 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| fructan biosynthesis | RXN-1781 | EC-2.4.1.99 | sucrose:sucrose fructosyltransferase |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
