| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra019976 |
| Family | PL4 |
| Protein Properties | Length: 675 Molecular Weight: 76995 Isoelectric Point: 6.6415 |
| Chromosome | Chromosome/Scaffold: 06 Start: 3409270 End: 3413008 |
| Description | Rhamnogalacturonate lyase family protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| PL4 | 49 | 656 | 0 |
| VRLHISDRYVVMDNGILQVTLSNPGGIITGINYNGVDNVLEVRNKETNRGYWDLHWNEPGGKGIFDVISGESFRVIVETEEQVEISFLRTWNPSLEGKYI PLNIDKRFIMLRGSSGVYSYGIYEHLKEWPGFELGETRIAFKLRKDKFHYMAVADDRKRIMPFPDDLCKGRCQTLDYQEASLLTAPCDPHLQGEVDDKYQ YSCENKDLRVHGWISFDPPVGFWQITPSNEFRSGGPLKQNLTSHVGPTTLAVFHSTHYAGKTMMPRFECGEPWKKVYGPVFIYLNSTANRDDSLFLWDDA KIKMMAEVESWPYSFVASEDYPKSEERGMARGRLLVCDRFVSNDLISAKGAYVGLAPPGDAGSWQIECKGYQFWAITDEAGYFSIDNVRPGEYNLYAWVP GFIGDYHNNIVVSVTSGCKIELGDIVYEPPRDGPTLWEIGIPDRKASEFFIPDPDPTLVNRVLVHPQDRFRQYGLWKRYTDLYPNDDLVYTIGVSDYRRD WFFAHVPRKKGDVYEGTTWQIRFKLENVDQKANYKLRVAIASATLAELQVRVNDADATRPLFTTGLIGRDNSIARHGVHGVYMLYTVNIPGNRFVQGDNT IFLKQPRC | |||
| Full Sequence |
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| Protein Sequence Length: 675 Download |
| MKKLADRLLH RLTNHETASN ADHHCSTGTG EGTSSLRHRQ DRRMPLQGVR LHISDRYVVM 60 DNGILQVTLS NPGGIITGIN YNGVDNVLEV RNKETNRGYW DLHWNEPGGK GIFDVISGES 120 FRVIVETEEQ VEISFLRTWN PSLEGKYIPL NIDKRFIMLR GSSGVYSYGI YEHLKEWPGF 180 ELGETRIAFK LRKDKFHYMA VADDRKRIMP FPDDLCKGRC QTLDYQEASL LTAPCDPHLQ 240 GEVDDKYQYS CENKDLRVHG WISFDPPVGF WQITPSNEFR SGGPLKQNLT SHVGPTTLAV 300 FHSTHYAGKT MMPRFECGEP WKKVYGPVFI YLNSTANRDD SLFLWDDAKI KMMAEVESWP 360 YSFVASEDYP KSEERGMARG RLLVCDRFVS NDLISAKGAY VGLAPPGDAG SWQIECKGYQ 420 FWAITDEAGY FSIDNVRPGE YNLYAWVPGF IGDYHNNIVV SVTSGCKIEL GDIVYEPPRD 480 GPTLWEIGIP DRKASEFFIP DPDPTLVNRV LVHPQDRFRQ YGLWKRYTDL YPNDDLVYTI 540 GVSDYRRDWF FAHVPRKKGD VYEGTTWQIR FKLENVDQKA NYKLRVAIAS ATLAELQVRV 600 NDADATRPLF TTGLIGRDNS IARHGVHGVY MLYTVNIPGN RFVQGDNTIF LKQPRCNGPF 660 QGIMYDYIRL EGSP* 720 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam13620 | CarboxypepD_reg | 5.0e-5 | 423 | 462 | 40 | + Carboxypeptidase regulatory-like domain. | ||
| cd10316 | RGL4_M | 5.0e-30 | 374 | 473 | 100 | + Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10317 | RGL4_C | 7.0e-52 | 485 | 671 | 189 | + C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10320 | RGL4_N | 1.0e-83 | 49 | 335 | 293 | + N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| pfam06045 | Rhamnogal_lyase | 2.0e-104 | 38 | 243 | 206 | + Rhamnogalacturonate lyase family. Rhamnogalacturonate lyase (EC:4.2.2.-) degrades the rhamnogalacturonan I (RG-I) backbone of pectin. This family contains mainly members from plants, but also contains the plant pathogen Erwinia chrysanthemi. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAB60743.1 | 0 | 44 | 674 | 1 | 727 | F21M12.30 gene product [Arabidopsis thaliana] |
| RefSeq | NP_172462.2 | 0 | 1 | 674 | 1 | 674 | lyase [Arabidopsis thaliana] |
| RefSeq | XP_002306520.1 | 0 | 60 | 674 | 1 | 616 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002527352.1 | 0 | 44 | 672 | 1 | 630 | lyase, putative [Ricinus communis] |
| RefSeq | XP_002527353.1 | 0 | 44 | 674 | 1 | 634 | lyase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1nkg_A | 0.001 | 395 | 652 | 255 | 484 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus |
| PDB | 3njv_A | 0.001 | 395 | 652 | 255 | 484 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus |
| PDB | 2xhn_B | 0.001 | 395 | 652 | 255 | 484 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
| PDB | 2xhn_A | 0.001 | 395 | 652 | 255 | 484 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
| PDB | 3njx_A | 0.001 | 395 | 652 | 255 | 484 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus Mutant H210a |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DK517187 | 242 | 434 | 675 | 0 |
| CN826718 | 235 | 270 | 500 | 0 |
| HO206588 | 225 | 164 | 388 | 0 |
| CD828788 | 238 | 1 | 238 | 0 |
| CN826718 | 21 | 490 | 510 | 0.11 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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