| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra020646 |
| Family | GH3 |
| Protein Properties | Length: 1226 Molecular Weight: 134976 Isoelectric Point: 8.1967 |
| Chromosome | Chromosome/Scaffold: 02 Start: 23964056 End: 23971296 |
| Description | beta-xylosidase 1 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH3 | 81 | 329 | 0 |
| AAVPRLGIGGYEWWSEALHGVSDVGPGAKFGGAFPGATSFPQVITTAASFNQSLWEEIGRVVSDEARAMYNGGVAGLTYWSPNVNILRDPRWGRGQETPG EDPVVAGKYAASYVRGLQGNGAGNRLKVAACCKHYTAYDLDNWNGVDRFHFNAKVTKQDLEDTYNVPFKSCVYEGKVASVMCSYNQVNGKPTCADENLLK NTIRGQWRLNGYIVSDCDSVDVFFNQQHYTTTPEEAAAASIKAGLDLDC | |||
| Full Sequence |
|---|
| Protein Sequence Length: 1226 Download |
| MFCNNNSLLT GNKVVGVLVF LLCLVHSSES LRPLFACDPA NGLTRTLRFC RVNVPVHARV 60 QDLIGRLSLQ EKIRLLVNNA AAVPRLGIGG YEWWSEALHG VSDVGPGAKF GGAFPGATSF 120 PQVITTAASF NQSLWEEIGR VVSDEARAMY NGGVAGLTYW SPNVNILRDP RWGRGQETPG 180 EDPVVAGKYA ASYVRGLQGN GAGNRLKVAA CCKHYTAYDL DNWNGVDRFH FNAKVTKQDL 240 EDTYNVPFKS CVYEGKVASV MCSYNQVNGK PTCADENLLK NTIRGQWRLN GYIVSDCDSV 300 DVFFNQQHYT TTPEEAAAAS IKAGLDLDCG PFLAIFTEGA VKKGLLTEND VNLALANTIT 360 VQMRLGMFDG NLGPYANLGP RDVCTPAHQH LALEAAHQGI VLLKNSGRSL PLSPRRHRTI 420 AVIGPNSDVT ETMIGNYAGK ACAYTSPLQG ISRYAKTLHQ EGCAGVACAG SQGFGAAEAA 480 ARQADATVLV MGLDQSIEAE TRDRTGLLLP GYQQNLVTRV AQASRGPVIL VLMSGGPVDV 540 SFAKNDPRVA AIIWAGYPGQ AGGAAIADII FGAANPGGKL PMTWYPQDYV TKLPMTIMAM 600 RASGNYPGRT YRFYKGLVVF PFGFGLSYTT FTHTLAQSPL AQLSVSPYKL NTAIFNSSSN 660 SIKVSHANCG TFPKMPLHVE VSNTGELDGT HTVFVFAEPP ENGIKGLGVN KQLVAFEKVH 720 VTAGSKRTVQ VDIEACKHLG VVDEHGMRRI PMGEHKLHIE TRTKKIVRDV TFTMADLKNL 780 FLITKHPSTT QILLTSLFFI SLFLLSSSPL PDFSHSLIVS SFTSRLLTAA NFFSSSSASD 840 SNTLYSVSPS SIRVNNESKK MDKELTSCDI FDGAWVSDDS DPVYLPGYCP YVEDKFNCFK 900 NGRPDSGFLR HRWQPHGCSV PRFDGEKMLE ILRGKRLVFV GDSLNRNMWE SLVCSLRSTL 960 EDKNRVSRVF VKQSNLHNEG FYGFRFKDFE CSIDFIKSPF LVQESEVLDA YGKRKETLRL 1020 DVIQESITKI YRNADIVVFN TGHWWTHQKT NEGKDYFQEG DRVYEKLEVK EAYTKALRTW 1080 ADWVDSSINS TRTRVFFVGY SSSHFRKGAW NAGGQCDGET RPIENETYTG GYPWMMKVVE 1140 SVISDMKTPV FYMNITKMTW YRTDGHPSVY RQPVDARGSS PASGMFQDCS HWCLPGVPDS 1200 WNQLLYATLL VSSGSLPYRS LGTLL* 1260 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PRK15098 | PRK15098 | 4.0e-58 | 118 | 737 | 682 | + beta-D-glucoside glucohydrolase; Provisional | ||
| pfam00933 | Glyco_hydro_3 | 3.0e-61 | 63 | 361 | 305 | + Glycosyl hydrolase family 3 N terminal domain. | ||
| PLN02629 | PLN02629 | 5.0e-101 | 866 | 1210 | 352 | + powdery mildew resistance 5 | ||
| pfam13839 | PC-Esterase | 4.0e-109 | 921 | 1209 | 291 | + GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p. The PC-Esterase family is comprised of Cas1p, the Homo sapiens C7orf58, Arabidopsis thaliana PMR5 and a group of plant freezing resistance/coldacclimatization proteins typified by Arabidopsis thaliana ESKIMO1, animal FAM55D proteins, and animal FAM113 proteins. The PC-Esterase family has features that are both similar and different from the canonical GDSL/SGNH superfamily. The members of this family are predicted to have Acyl esterase activity and predicted to modify cell-surface biopolymers such as glycans and glycoproteins. The Cas1p protein has a Cas1_AcylT domain, in addition, with the opposing acyltransferase activity. The C7orf58 family has a ATP-Grasp domain fused to the PC-Esterase and is the first identified secreted tubulin-tyrosine ligase like enzyme in eukaryotes. The plant family with PMR5, ESK1, TBL3 etc have a N-terminal C rich potential sugar binding domain followed by the PC-Esterase domain. | ||
| PLN03080 | PLN03080 | 0 | 27 | 757 | 751 | + Probable beta-xylosidase; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAS17751.2 | 0 | 5 | 759 | 6 | 754 | beta xylosidase [Fragaria x ananassa] |
| DDBJ | BAG28345.1 | 0 | 32 | 751 | 25 | 743 | arabinofuranosidase [Citrus unshiu] |
| RefSeq | NP_199747.1 | 0 | 1 | 759 | 1 | 761 | BXL1 (BETA-XYLOSIDASE 1); hydrolase, hydrolyzing O-glycosyl compounds [Arabidopsis thaliana] |
| RefSeq | XP_002316021.1 | 0 | 26 | 759 | 20 | 750 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002519194.1 | 0 | 26 | 759 | 34 | 764 | Periplasmic beta-glucosidase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3u48_B | 0 | 118 | 733 | 91 | 696 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
| PDB | 3u48_A | 0 | 118 | 733 | 91 | 696 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
| PDB | 3u4a_B | 0 | 118 | 733 | 91 | 696 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
| PDB | 3u4a_A | 0 | 118 | 733 | 91 | 696 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
| PDB | 2x41_A | 3e-39 | 59 | 634 | 3 | 606 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| (1,4)-β-xylan degradation | 3.2.1.37-RXN | EC-3.2.1.37 | xylan 1,4-β-xylosidase |
