| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra021930 |
| Family | AA7 |
| Protein Properties | Length: 503 Molecular Weight: 56001.4 Isoelectric Point: 8.5226 |
| Chromosome | Chromosome/Scaffold: 04 Start: 15250426 End: 15253867 |
| Description | FAD-binding Berberine family protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 70 | 306 | 0 |
| QNLRYLTQSNPKPVFIFEPLYETHVQAAVVCAKKLQLHMRLRSGGHDYEGLSFVAENVTPFVVVDLSKLRQIDVDLDSNSAWAHAGATVGEVYYRIQEKS QTHGFPAGLCSSLGIGGHLVGGAYGSMMRKFGLGADNVLDARIIDANGKILDRAAMGEDVFWAIRGAGGGSFGVILAWKIKLVPVPATVTVFTVTKTLEQ DGTNVLYKWQQVADKLDEDLFIRVIIQPAGKTTKTGN | |||
| Full Sequence |
|---|
| Protein Sequence Length: 503 Download |
| MAFAISTRNA VAILVTLLLV SARPSSSTLQ QDFIHCLVDN TDMAFPIRAS FFSPDQNATL 60 FKEELESTAQ NLRYLTQSNP KPVFIFEPLY ETHVQAAVVC AKKLQLHMRL RSGGHDYEGL 120 SFVAENVTPF VVVDLSKLRQ IDVDLDSNSA WAHAGATVGE VYYRIQEKSQ THGFPAGLCS 180 SLGIGGHLVG GAYGSMMRKF GLGADNVLDA RIIDANGKIL DRAAMGEDVF WAIRGAGGGS 240 FGVILAWKIK LVPVPATVTV FTVTKTLEQD GTNVLYKWQQ VADKLDEDLF IRVIIQPAGK 300 TTKTGNRTIS TSYQGQFLGD SNRLMQVMQK SFPELGLTKK DCIEMSWIKS VMYIAGFPNS 360 APPEALLAGK SLFKNHFKAK SDFVEEPIPV EGLEGLWKKL LKEDSPLTIW NPYGGMMSRI 420 SESETPFPHR KGTLFKIQWL STWSDGKASE ARHIEWMRDM YSYMEQYVSK KPRRAYVNYR 480 VMLESGGLKY SDKDWLMELG GR* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG0812 | MurB | 0.0006 | 123 | 226 | 113 | + UDP-N-acetylmuramate dehydrogenase [Cell envelope biogenesis, outer membrane] | ||
| TIGR01679 | bact_FAD_ox | 0.0003 | 82 | 244 | 166 | + FAD-linked oxidoreductase. This model represents a family of bacterial oxidoreductases with covalently linked FAD, closely related to two different eukaryotic oxidases, L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae. | ||
| COG0277 | GlcD | 8.0e-18 | 94 | 251 | 167 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 2.0e-25 | 82 | 221 | 141 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0016491 | oxidoreductase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAD25763.1 | 0 | 27 | 429 | 29 | 431 | AC007060_21 Strong similarity to F19I3.2 gi |
| RefSeq | NP_174363.1 | 0 | 27 | 480 | 29 | 482 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| RefSeq | NP_181025.1 | 0 | 1 | 480 | 1 | 480 | MEE23 (MATERNAL EFFECT EMBRYO ARREST 23); FAD binding / catalytic/ electron carrier/ oxidoreductase [Arabidopsis thaliana] |
| RefSeq | XP_002523162.1 | 0 | 33 | 480 | 33 | 477 | Reticuline oxidase precursor, putative [Ricinus communis] |
| RefSeq | XP_002523164.1 | 0 | 33 | 480 | 33 | 477 | Reticuline oxidase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 4dns_B | 0 | 24 | 480 | 4 | 440 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 4dns_A | 0 | 24 | 480 | 4 | 440 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 3vte_A | 0 | 30 | 480 | 4 | 458 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 3tsj_B | 0 | 30 | 480 | 8 | 440 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 3tsj_A | 0 | 30 | 480 | 8 | 440 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cannabinoid biosynthesis | RXN-7854 | EC-1.21.3 | tetrahydrocannabinolic acid synthase |
