| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra022342 |
| Family | GT8 |
| Protein Properties | Length: 631 Molecular Weight: 72819.3 Isoelectric Point: 8.5953 |
| Chromosome | Chromosome/Scaffold: 05 Start: 18300466 End: 18304058 |
| Description | plant glycogenin-like starch initiation protein 1 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT8 | 324 | 508 | 1.3e-37 |
| TILHSAHVYVCGAIAAAQSIRQSGSTRDLVILVDENISGYHRSGLEAAGWQIRTIQRIRNPKAEKDAYNEWNYSKFRLWQLTDYNKIIFIDADLLILRNI DFLFSMPEISATGNNGTLFNSGVMVIEPCNCTFQLLMEHINEIESYNGGDQGYLNEVFTWWHRIPKHMNFLKHFWVGDEDDVRRK | |||
| Full Sequence |
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| Protein Sequence Length: 631 Download |
| MANPPAAAVA TSATGDTRRR LSASISEAIC KRRFRRNSKS GGRSDMVKPF NILNFPTQDK 60 NSSCCCSVFQ IVKVLLCILL SATLFTIIYS PEAYHHSLSH SSRWIWRRQD PRYVSDLEIS 120 WDDVTKTIES VQEGRTIGVL NFDTKEIRRW RDVAKTKDNE DDENVVELDL EHADKNVTWD 180 ALYPEWIDEE QEKDVPVCPN LPKIKVPTRR LDLVVVKLPC RKEGNWSRDV ARLHLQMAAA 240 TVAVSAKGFF RGHVMFVSRC FPIPNLFRCK DLVSRRGNVW LYKPNLDTLR DKLQLPIGSC 300 ELSLPLGIKE RPTLGNPKRE TYATILHSAH VYVCGAIAAA QSIRQSGSTR DLVILVDENI 360 SGYHRSGLEA AGWQIRTIQR IRNPKAEKDA YNEWNYSKFR LWQLTDYNKI IFIDADLLIL 420 RNIDFLFSMP EISATGNNGT LFNSGVMVIE PCNCTFQLLM EHINEIESYN GGDQGYLNEV 480 FTWWHRIPKH MNFLKHFWVG DEDDVRRKKT ELFGAEPPIL YFATDIAHRR WWMVHDAMPQ 540 ELHQFCYLRS KQKAQLEYDR RQAEAANYAD GHWKIRVKDP RFKICIDKLC NWKSMLRHWG 600 ESNWTDYESF VPTPPAITAV RKTSLPGHNL * 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00505 | Glyco_transf_8 | 4.0e-10 | 325 | 494 | 207 | + Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+. | ||
| cd06914 | GT8_GNT1 | 7.0e-15 | 332 | 482 | 164 | + GNT1 is a fungal enzyme that belongs to the GT 8 family. N-acetylglucosaminyltransferase is a fungal enzyme that catalyzes the addition of N-acetyl-D-glucosamine to mannotetraose side chains by an alpha 1-2 linkage during the synthesis of mannan. The N-acetyl-D-glucosamine moiety in mannan plays a role in the attachment of mannan to asparagine residues in proteins. The mannotetraose and its N-acetyl-D-glucosamine derivative side chains of mannan are the principle immunochemical determinants on the cell surface. N-acetylglucosaminyltransferase is a member of glycosyltransferase family 8, which are, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed, retaining glycosyltransferases. | ||
| PLN00176 | PLN00176 | 5.0e-15 | 301 | 430 | 133 | + galactinol synthase | ||
| pfam01501 | Glyco_transf_8 | 2.0e-37 | 323 | 525 | 246 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd02537 | GT8_Glycogenin | 1.0e-60 | 322 | 498 | 186 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAX33317.1 | 0 | 1 | 627 | 1 | 635 | secondary cell wall-related glycosyltransferase family 8 [Populus tremula x Populus tremuloides] |
| RefSeq | NP_001030722.1 | 0 | 15 | 630 | 16 | 659 | PGSIP1 (PLANT GLYCOGENIN-LIKE STARCH INITIATION PROTEIN 1); transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | NP_566615.1 | 0 | 15 | 630 | 16 | 655 | PGSIP1 (PLANT GLYCOGENIN-LIKE STARCH INITIATION PROTEIN 1); transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002310513.1 | 0 | 10 | 627 | 3 | 629 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002517237.1 | 0 | 12 | 627 | 8 | 629 | glycogenin, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3u2w_B | 2e-20 | 326 | 494 | 10 | 187 | A Chain A, Crystal Structure Of The Legionella Pneumophila Gap Domain Of Lepb In Complex With Rab1b Bound To Gdp And Bef3 |
| PDB | 3u2w_A | 2e-20 | 326 | 494 | 10 | 187 | A Chain A, Crystal Structure Of The Legionella Pneumophila Gap Domain Of Lepb In Complex With Rab1b Bound To Gdp And Bef3 |
| PDB | 3qvb_A | 2e-20 | 326 | 494 | 10 | 187 | A Chain A, Crystal Structure Of The Legionella Pneumophila Gap Domain Of Lepb In Complex With Rab1b Bound To Gdp And Bef3 |
| PDB | 3q4s_A | 2e-20 | 326 | 494 | 10 | 187 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1), Apo Form |
| PDB | 3u2x_B | 2e-20 | 326 | 494 | 10 | 187 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1), Apo Form |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| FG552677 | 315 | 224 | 536 | 0 |
| EE490483 | 247 | 208 | 454 | 0 |
| GT009571 | 265 | 253 | 517 | 0 |
| JG942762 | 273 | 246 | 518 | 0 |
| FG552677 | 40 | 512 | 551 | 6.7 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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