| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra022570 |
| Family | AA3 |
| Protein Properties | Length: 535 Molecular Weight: 59074.2 Isoelectric Point: 6.7618 |
| Chromosome | Chromosome/Scaffold: 02 Start: 8847883 End: 8849739 |
| Description | Glucose-methanol-choline (GMC) oxidoreductase family protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA3 | 7 | 529 | 0 |
| APTHAHFDYIVIGGGTAGCALATTLSQNANVLVLERGGSPYDNPTATDLENFTNTLFNITPDSWSQPFISEDGVYNVRARVLGGESVLNAGFYSRAEEYY VEYAEWERDEVEAAYKWVESKLVYEPQVTGWQSALKHGLLEAGVLPYNGFTLDHIVGTKISGTIFDPAGHRHSAADLLEYANPDTIVVYLHALVHKILFT TKGGQRPRAYGVIYQDADGVFHKAELAKNAMSEVILCAGAIASPQLLMLSGVGPRAHLEAHGVNPVVLDHPMVGQGMGDNPLNTVFVSSPRPVETSLIKV VGITNFDTFIEGFSGLKLTNNLMHRFSEGILNETSRSTLAITEFFKSIDLRSNVLEIDGWIVNKVDGPVSRGHLELRNKNPDDNPSVRFNYYQEPEDLEK CVKGLKTIIKVINSNAFSSYKYPNATARELLNRMLTLPINLRPRHESAKFNLTQFCIDTVITIWHYHGGCQVGRVVDKNYRVLGIDALRVIDGSTFLKAP GTNPQATVMMLGRYMGKKILRER | |||
| Full Sequence |
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| Protein Sequence Length: 535 Download |
| MKDATLAPTH AHFDYIVIGG GTAGCALATT LSQNANVLVL ERGGSPYDNP TATDLENFTN 60 TLFNITPDSW SQPFISEDGV YNVRARVLGG ESVLNAGFYS RAEEYYVEYA EWERDEVEAA 120 YKWVESKLVY EPQVTGWQSA LKHGLLEAGV LPYNGFTLDH IVGTKISGTI FDPAGHRHSA 180 ADLLEYANPD TIVVYLHALV HKILFTTKGG QRPRAYGVIY QDADGVFHKA ELAKNAMSEV 240 ILCAGAIASP QLLMLSGVGP RAHLEAHGVN PVVLDHPMVG QGMGDNPLNT VFVSSPRPVE 300 TSLIKVVGIT NFDTFIEGFS GLKLTNNLMH RFSEGILNET SRSTLAITEF FKSIDLRSNV 360 LEIDGWIVNK VDGPVSRGHL ELRNKNPDDN PSVRFNYYQE PEDLEKCVKG LKTIIKVINS 420 NAFSSYKYPN ATARELLNRM LTLPINLRPR HESAKFNLTQ FCIDTVITIW HYHGGCQVGR 480 VVDKNYRVLG IDALRVIDGS TFLKAPGTNP QATVMMLGRY MGKKILRERN AWGK* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PRK02106 | PRK02106 | 7.0e-22 | 169 | 285 | 123 | + choline dehydrogenase; Validated | ||
| pfam05199 | GMC_oxred_C | 9.0e-26 | 374 | 518 | 150 | + GMC oxidoreductase. This domain found associated with pfam00732. | ||
| TIGR01810 | betA | 3.0e-31 | 14 | 526 | 569 | + choline dehydrogenase. Choline dehydrogenase catalyzes the conversion of exogenously supplied choline into the intermediate glycine betaine aldehyde, as part of a two-step oxidative reaction leading to the formation of osmoprotectant betaine. This enzymatic system can be found in both gram-positive and gram-negative bacteria. As in Escherichia coli , Staphylococcus xylosus , and Sinorhizobium meliloti, this enzyme is found associated in a transciptionally co-induced gene cluster with betaine aldehyde dehydrogenase, the second catalytic enzyme in this reaction. Other gram-positive organisms have been shown to employ a different enzymatic system, utlizing a soluable choline oxidase or type III alcohol dehydrogenase instead of choline dehydrogenase. This enzyme is a member of the GMC oxidoreductase family (pfam00732 and pfam05199), sharing a common evoluntionary origin and enzymatic reaction with alcohol dehydrogenase. Outgrouping from this model, Caulobacter crescentus shares sequence homology with choline dehydrogenase, yet other genes participating in this enzymatic reaction have not currently been identified [Cellular processes, Adaptations to atypical conditions]. | ||
| COG2303 | BetA | 3.0e-36 | 7 | 529 | 579 | + Choline dehydrogenase and related flavoproteins [Amino acid transport and metabolism] | ||
| PLN02785 | PLN02785 | 0 | 4 | 529 | 538 | + Protein HOTHEAD | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016614 | oxidoreductase activity, acting on CH-OH group of donors |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAB11043.1 | 0 | 1 | 519 | 34 | 563 | mandelonitrile lyase-like protein [Arabidopsis thaliana] |
| EMBL | CAB87405.1 | 0 | 1 | 519 | 34 | 557 | ADHESION OF CALYX EDGES-like protein [Arabidopsis thaliana] |
| RefSeq | NP_001119417.1 | 0 | 1 | 534 | 1 | 548 | glucose-methanol-choline (GMC) oxidoreductase family protein [Arabidopsis thaliana] |
| RefSeq | NP_200008.1 | 0 | 1 | 534 | 34 | 581 | glucose-methanol-choline (GMC) oxidoreductase family protein [Arabidopsis thaliana] |
| RefSeq | NP_567032.1 | 0 | 1 | 532 | 34 | 570 | glucose-methanol-choline (GMC) oxidoreductase family protein [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3gdp_B | 0 | 3 | 531 | 17 | 519 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
| PDB | 3gdp_A | 0 | 3 | 531 | 17 | 519 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
| PDB | 3gdn_B | 0 | 3 | 531 | 17 | 519 | A Chain A, Almond Hydroxynitrile Lyase In Complex With Benzaldehyde |
| PDB | 3gdn_A | 0 | 3 | 531 | 17 | 519 | A Chain A, Almond Hydroxynitrile Lyase In Complex With Benzaldehyde |
| PDB | 1ju2_B | 0 | 3 | 531 | 17 | 519 | A Chain A, Crystal Structure Of The Hydroxynitrile Lyase From Almond |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cutin biosynthesis | RXN-2121 | EC-1.1.1 | 18-hydroxyoleate dehydrogenase |
| cutin biosynthesis | RXN-9802 | EC-1.1.1 | 16-hydroxypalmitate dehydrogenase |
| suberin biosynthesis | RXN-2121 | EC-1.1.1 | 18-hydroxyoleate dehydrogenase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO796743 | 528 | 14 | 529 | 0 |
| FG564536 | 217 | 114 | 330 | 0 |
| EX050677 | 249 | 57 | 305 | 0 |
| FG568453 | 205 | 1 | 205 | 0 |
| CD827024 | 201 | 1 | 201 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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