| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra023053 |
| Family | GH32 |
| Protein Properties | Length: 588 Molecular Weight: 67024 Isoelectric Point: 8.8885 |
| Chromosome | Chromosome/Scaffold: 03 Start: 8421213 End: 8424307 |
| Description | cell wall invertase 4 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 52 | 368 | 0 |
| HFQPPKHWINGPLYYKGFYHLFYQYNTKGAVWGNIVWAHSVSKDLVNWEALEHAIYPSKWFDIKGTWSGSITLVPGKGPVILYTGINQNETQIQNYAIPK DPSDPYLRKWIKPDDNPIAVPDYTMNGSDFRDPTTAWFSKDGHWRTVVGSRRKHRGIGYIYRSKDFKHWVKAKHPIHSKESTGMWECPDFFPVSTTDFEN GLDLDYAGPNAKHVLKVSLDMTRYEYYTLGKYYPNKDRYVPNGESPDGWDGLRFDYGNFYASKTFFDYKKNRRILWGWANESDTVEDDISKGWAGLQVIP RTVLLDSTNKKQLVFWP | |||
| Full Sequence |
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| Protein Sequence Length: 588 Download |
| MGVSSIISVM LLLVLINLGN NIKGIEAFHQ VYEKLQSQSV ESVNHLHRSA FHFQPPKHWI 60 NGPLYYKGFY HLFYQYNTKG AVWGNIVWAH SVSKDLVNWE ALEHAIYPSK WFDIKGTWSG 120 SITLVPGKGP VILYTGINQN ETQIQNYAIP KDPSDPYLRK WIKPDDNPIA VPDYTMNGSD 180 FRDPTTAWFS KDGHWRTVVG SRRKHRGIGY IYRSKDFKHW VKAKHPIHSK ESTGMWECPD 240 FFPVSTTDFE NGLDLDYAGP NAKHVLKVSL DMTRYEYYTL GKYYPNKDRY VPNGESPDGW 300 DGLRFDYGNF YASKTFFDYK KNRRILWGWA NESDTVEDDI SKGWAGLQVI PRTVLLDSTN 360 KKQLVFWPIE EIESLRSSYV RMNNKDIKMG QRLEVKGITP AQADVEVTFN VGSLEKAEPF 420 DRSYTYKPLD LCRVKGSNVT GGVGPFGLIT FATPDLEEYT PVFFRVFKDT STNKPKVLMC 480 SDARPSSLKQ DRGPLKQDRM YKPPFAGFVD VDLADGRISL RSLIDHSVVE SFGALGKTVI 540 TSRVYPIKAV KENAHLYVFN NGTQTVNVES LDAWSMERPL EMNNGAT* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG1621 | SacC | 1.0e-53 | 47 | 395 | 370 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 2.0e-79 | 58 | 371 | 329 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-135 | 52 | 368 | 328 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 1.0e-163 | 52 | 536 | 497 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM61359.1 | 0 | 20 | 586 | 22 | 590 | putative beta-fructofuranosidase (invertase) [Arabidopsis thaliana] |
| GenBank | ABK28599.1 | 0 | 9 | 586 | 9 | 589 | unknown [Arabidopsis thaliana] |
| EMBL | CAB43403.1 | 0 | 9 | 586 | 9 | 586 | beta-fructofuranosidase [Arabidopsis thaliana] |
| RefSeq | NP_190828.2 | 0 | 9 | 586 | 9 | 589 | AtcwINV2 (Arabidopsis thaliana cell wall invertase 2); hydrolase, hydrolyzing O-glycosyl compounds |
| RefSeq | NP_565837.1 | 0 | 20 | 586 | 22 | 590 | AtcwINV4 (Arabidopsis thaliana cell wall invertase 4); hydrolase, hydrolyzing O-glycosyl compounds |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 42 | 576 | 3 | 535 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2qqw_A | 0 | 44 | 576 | 1 | 531 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D23a) From Arabidopsis Thaliana In Complex With Sucrose |
| PDB | 2xqr_K | 0 | 44 | 576 | 1 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 44 | 576 | 1 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 44 | 576 | 1 | 531 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
