| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra023123 |
| Family | AA7 |
| Protein Properties | Length: 513 Molecular Weight: 57259.1 Isoelectric Point: 9.554 |
| Chromosome | Chromosome/Scaffold: 09 Start: 21542898 End: 21544551 |
| Description | FAD-binding Berberine family protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 69 | 187 | 3.8e-36 |
| RNLRYFNNTTRKPIAIVAAADVTHIQATITCAKKLSLQLRIRSGGHDYDGLSYLSTVDFVVLDMFNLRSIEFGRKLDTAWVQSGATLGEIYYGVANKSND LRGFPAGICPGLGAGGHFS | |||
| AA7 | 188 | 506 | 3.1e-36 |
| DAKIVDAKGRVLDRTSMGEDLFWALRGGGAASFCVVLAWKIKLVPVPEKVTVFNVEAVGKRGGVNTKELVVKWQEIADKIDNSLFIRLTLSTSNKTVKAS FMGMFLGDSSRLLEIMTKEFPELGLNKTECIEMKWIESVLFWLSIPPGTAPTSVMLNRIPQKQIYLKRKSDYVQKPISRTGLDAIFKVLLENENVTMAWN PYGGRMSEIPSTETAFPHRAGNKFKIQYAANWFVPGEEVAKECMSQTERVFEAMTPYVSKNPREAFLNYRDVDIGTSLNSTYEEGKVYGVKYFKNNFERL VSVKSRVDPDNFFRYEQSI | |||
| Full Sequence |
|---|
| Protein Sequence Length: 513 Download |
| MKSPTIQTLI ISVFLFTIQT SLASPPTLGS VFTQCIIGLK PSNPCSPIQN FTYTPQNPKF 60 LIILNNYVRN LRYFNNTTRK PIAIVAAADV THIQATITCA KKLSLQLRIR SGGHDYDGLS 120 YLSTVDFVVL DMFNLRSIEF GRKLDTAWVQ SGATLGEIYY GVANKSNDLR GFPAGICPGL 180 GAGGHFSDAK IVDAKGRVLD RTSMGEDLFW ALRGGGAASF CVVLAWKIKL VPVPEKVTVF 240 NVEAVGKRGG VNTKELVVKW QEIADKIDNS LFIRLTLSTS NKTVKASFMG MFLGDSSRLL 300 EIMTKEFPEL GLNKTECIEM KWIESVLFWL SIPPGTAPTS VMLNRIPQKQ IYLKRKSDYV 360 QKPISRTGLD AIFKVLLENE NVTMAWNPYG GRMSEIPSTE TAFPHRAGNK FKIQYAANWF 420 VPGEEVAKEC MSQTERVFEA MTPYVSKNPR EAFLNYRDVD IGTSLNSTYE EGKVYGVKYF 480 KNNFERLVSV KSRVDPDNFF RYEQSIPVPS SH* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG0277 | GlcD | 3.0e-6 | 64 | 242 | 207 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 3.0e-14 | 81 | 200 | 139 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| pfam08031 | BBE | 6.0e-17 | 452 | 507 | 56 | + Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0016491 | oxidoreductase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAF79255.1 | 0 | 1 | 512 | 1 | 527 | AC023279_4 F12K21.9 [Arabidopsis thaliana] |
| RefSeq | NP_174358.1 | 0 | 1 | 512 | 1 | 531 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| RefSeq | NP_564449.1 | 0 | 1 | 512 | 1 | 527 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| RefSeq | XP_002317086.1 | 0 | 1 | 511 | 1 | 528 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002523155.1 | 0 | 32 | 507 | 29 | 522 | Reticuline oxidase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 4dns_B | 0 | 53 | 507 | 27 | 494 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 4dns_A | 0 | 53 | 507 | 27 | 494 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 3vte_A | 0 | 32 | 512 | 7 | 517 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 3tsj_B | 0 | 25 | 507 | 4 | 494 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 3tsj_A | 0 | 25 | 507 | 4 | 494 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cannabinoid biosynthesis | RXN-7854 | EC-1.21.3 | tetrahydrocannabinolic acid synthase |
