| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra023127 |
| Family | AA7 |
| Protein Properties | Length: 397 Molecular Weight: 44944.5 Isoelectric Point: 9.9486 |
| Chromosome | Chromosome/Scaffold: 09 Start: 21530319 End: 21531509 |
| Description | FAD-binding Berberine family protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 5 | 388 | 0 |
| GGSAWVQTGATLGELFFRIWEKSEVHAFPAGICPTVGVGGHVSGGGYGHMIRKFGLTIDYVVDATIVDVNGRVLDRKSMGEDLFWALRGGGAGSFGVVLA FKVKLVEVPKTVTVFRIDRTTDQNALDMVYKWQFVAPRTDPGLFIRVLLASPTKNKTQTVNAKVRALYLGNASDVVSMMAKEFPELGLKMDDCKEMTWIQ SLLWWMNYKDVEKVKPEVLLEREPDKAKFVKRKSDYVEKEITKPELNRLIQQLATLDRTGLVLNPYGGNLNATATNETAFPHRHKLYKIQHSSTWSDARP EADRLYVGSLRTTYRIMTPFVSKNPRSAYLNYRDVDIGVNDHGKDSYRKGENYGRKYFGENFDRLVRVKTAVDPDNFFRNEQSI | |||
| Full Sequence |
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| Protein Sequence Length: 397 Download |
| MKDDGGSAWV QTGATLGELF FRIWEKSEVH AFPAGICPTV GVGGHVSGGG YGHMIRKFGL 60 TIDYVVDATI VDVNGRVLDR KSMGEDLFWA LRGGGAGSFG VVLAFKVKLV EVPKTVTVFR 120 IDRTTDQNAL DMVYKWQFVA PRTDPGLFIR VLLASPTKNK TQTVNAKVRA LYLGNASDVV 180 SMMAKEFPEL GLKMDDCKEM TWIQSLLWWM NYKDVEKVKP EVLLEREPDK AKFVKRKSDY 240 VEKEITKPEL NRLIQQLATL DRTGLVLNPY GGNLNATATN ETAFPHRHKL YKIQHSSTWS 300 DARPEADRLY VGSLRTTYRI MTPFVSKNPR SAYLNYRDVD IGVNDHGKDS YRKGENYGRK 360 YFGENFDRLV RVKTAVDPDN FFRNEQSIPT LPRKRR* 420 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam01565 | FAD_binding_4 | 0.0007 | 3 | 79 | 77 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| COG0277 | GlcD | 6.0e-11 | 56 | 391 | 357 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam08031 | BBE | 5.0e-19 | 332 | 389 | 58 | + Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0016491 | oxidoreductase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABK28425.1 | 0 | 1 | 396 | 138 | 533 | unknown [Arabidopsis thaliana] |
| GenBank | ACE79740.1 | 0 | 7 | 394 | 15 | 402 | At1g11770 [Arabidopsis thaliana] |
| DDBJ | BAD94511.1 | 0 | 1 | 394 | 132 | 528 | hypothetical protein [Arabidopsis thaliana] |
| RefSeq | NP_171700.1 | 0 | 1 | 394 | 141 | 537 | ATSEC1A; FAD binding / catalytic/ electron carrier/ oxidoreductase [Arabidopsis thaliana] |
| RefSeq | NP_174361.1 | 0 | 1 | 396 | 138 | 533 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3vte_A | 0 | 7 | 392 | 119 | 514 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 3tsj_B | 0 | 7 | 394 | 120 | 499 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 3tsj_A | 0 | 7 | 394 | 120 | 499 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 3tsh_A | 0 | 7 | 394 | 120 | 499 | A Chain A, Crystal Structure Of Phl P 4, A Grass Pollen Allergen With Glucose Dehydrogenase Activity |
| PDB | 4dns_B | 0 | 5 | 391 | 120 | 496 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cannabinoid biosynthesis | RXN-7854 | EC-1.21.3 | tetrahydrocannabinolic acid synthase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| FY430214 | 287 | 53 | 339 | 0 |
| EV532149 | 240 | 158 | 397 | 0 |
| EV528058 | 240 | 158 | 397 | 0 |
| EX886938 | 225 | 78 | 302 | 0 |
| DK566238 | 217 | 181 | 397 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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