| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra025117 |
| Family | CE10 |
| Protein Properties | Length: 305 Molecular Weight: 33250.3 Isoelectric Point: 5.1117 |
| Chromosome | Chromosome/Scaffold: 06 Start: 23357429 End: 23358590 |
| Description | alpha/beta-Hydrolases superfamily protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE10 | 36 | 278 | 0 |
| TPENGVVSKDTLFSPEHNLSVRIFLPEKASAATGEKLPLLVYFHGGGFVIETAFSPTYHTFLTTTAATADCLAVSVDYRRAPEFPIPVPYEDSWDALKWV LSHISGAGPEQWINKHADFGKLFLAGDSAGGNISHHLATRAKREGIDSVISGVVLIHPYFWGKAPVDEFETRDERKRKGVEARWRVASPNSEEGVDDPLF NVVGSESVDISGLGCGRVLVVVAGDDTFARQGLGYVAKLEKSG | |||
| Full Sequence |
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| Protein Sequence Length: 305 Download |
| MDSEIDVDNS PAYRVYKSGR IERLSGETFK PPSPLTPENG VVSKDTLFSP EHNLSVRIFL 60 PEKASAATGE KLPLLVYFHG GGFVIETAFS PTYHTFLTTT AATADCLAVS VDYRRAPEFP 120 IPVPYEDSWD ALKWVLSHIS GAGPEQWINK HADFGKLFLA GDSAGGNISH HLATRAKREG 180 IDSVISGVVL IHPYFWGKAP VDEFETRDER KRKGVEARWR VASPNSEEGV DDPLFNVVGS 240 ESVDISGLGC GRVLVVVAGD DTFARQGLGY VAKLEKSGWE GEVESPDTDN ARKVVNKVAE 300 FIQK* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00312 | Esterase_lipase | 6.0e-6 | 29 | 172 | 174 | + Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate. | ||
| PRK10162 | PRK10162 | 2.0e-9 | 50 | 195 | 147 | + acetyl esterase; Provisional | ||
| COG2272 | PnbA | 1.0e-9 | 29 | 166 | 170 | + Carboxylesterase type B [Lipid metabolism] | ||
| COG0657 | Aes | 3.0e-27 | 11 | 278 | 277 | + Esterase/lipase [Lipid metabolism] | ||
| pfam07859 | Abhydrolase_3 | 1.0e-54 | 75 | 284 | 216 | + alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAL57633.1 | 0 | 1 | 303 | 1 | 321 | AT3g48690/T8P19_200 [Arabidopsis thaliana] |
| GenBank | AAO41964.1 | 0 | 5 | 304 | 1 | 308 | putative esterase [Arabidopsis thaliana] |
| GenBank | AAT70485.1 | 0 | 13 | 304 | 2 | 301 | At2g03550 [Arabidopsis thaliana] |
| RefSeq | NP_178453.1 | 0 | 1 | 304 | 1 | 312 | hydrolase [Arabidopsis thaliana] |
| RefSeq | NP_190438.1 | 0 | 1 | 303 | 1 | 321 | CXE12; carboxylesterase [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2zsi_A | 1e-34 | 36 | 303 | 65 | 349 | B Chain B, Reassembly And Co-Crystallization Of A Family 9 Processive Endoglucanase From Separately Expressed Gh9 And Cbm3c Modules |
| PDB | 2zsh_A | 1e-34 | 36 | 303 | 65 | 349 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 2o7v_A | 3e-32 | 32 | 278 | 45 | 293 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 2o7r_A | 3e-32 | 32 | 278 | 45 | 293 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 3ed1_F | 3e-31 | 19 | 303 | 38 | 348 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| formononetin biosynthesis | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| formononetin biosynthesis | RXN-3625 | - | 2,7-dihydroxy-4'-methoxyisoflavanone dehydratase |
| isoflavonoid biosynthesis I | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-3303 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-5502 | - | 2,7,5-trihydroxy-4'-methoxyisoflavanone dehydratase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EX113610 | 256 | 1 | 256 | 0 |
| EV061344 | 248 | 27 | 274 | 0 |
| EX019947 | 283 | 1 | 278 | 0 |
| EV070914 | 206 | 59 | 264 | 0 |
| EX109246 | 278 | 1 | 273 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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