| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra025392 |
| Family | GT35 |
| Protein Properties | Length: 961 Molecular Weight: 108343 Isoelectric Point: 6.0621 |
| Chromosome | Chromosome/Scaffold: 06 Start: 21832854 End: 21837294 |
| Description | Glycosyl transferase, family 35 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT35 | 180 | 955 | 0 |
| ALKSLGFDLESVASQEPDPALGNGGLGRLASCFLDSMATLNYPAWGYGLRYKYGLFKQRITKDGQEEAAEDWLELSNPWEIVRNDVSYPVKFYGKVVSGS DGKKQWIGGEDIVAVAYDVPIPGYKTKTTINLRLWSTKAPSADFDLSSYNSGKHTEAAQALFNAEKICYVLYPGDESNEGKALRLKQQYTLCSASLQDII ARFETRSGGRVNWEEFPEKVAVQMNDTHPTLCIPELMRILMDLKGLSWEDAWKITQRTVAYTNHTVLPEALEKWSLDLMGKLLPRHVEIIEMIDEELVST IVSEYGTEDPDLLKEKLKAMRILENVELPSAFADVIVKPKKKTVTAKNTEESKEDAQTVVKKEQEEEVEVIPEPTVKLPKMVRMANLAVVGGHAVNGVAE IHSEIVKKDVFNEFVKLWPDKFQNKTNGVTPRRWIRFCNPYLSDIITNWIGTEDWVLNTEKLAELRKFADDEALQSEWRAAKKKNKLKVVSLIKERTGYT VNPDAMFDIQIKRIHEYKRQLLNILGIVYRYKKMKEMSASEREKAFVPRVCIFGGKAFATYLQAKRIVKFITDVGSTINNDPEIGDLLKVIFVPDYNVSV AELLIPASELSQHISTAGMEASGTSNMKFSMNGCILIGTLDGANVEIREEVGEENFFLFGAKADEIVNLRKERAEGKFVPDPIFEEVKQYVRSGVFGSNY DELIGSLEGNEGFGRADYFLVGKDFPSYVECQEKVDEAYRDQKRWTRMSILNTAGSFKFSSDRTIHEYAKDIWNIK | |||
| Full Sequence |
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| Protein Sequence Length: 961 Download |
| MDTMRISGVS SGAESLMHHN ALSSLVGRRC DNGRWRRTRM FPARSRTWRL SPKKTLFPSV 60 KAVSSEPKEK VADVVIDSEQ EGFSSLSPFG PDAASVASSI KYHSEFTPLF SPEKFELPKA 120 FFATAQSVRD ALIINWNATY EYYNRVNPKQ AYYLSMEFLQ GRALSNAVGN LGLTGAYADA 180 LKSLGFDLES VASQEPDPAL GNGGLGRLAS CFLDSMATLN YPAWGYGLRY KYGLFKQRIT 240 KDGQEEAAED WLELSNPWEI VRNDVSYPVK FYGKVVSGSD GKKQWIGGED IVAVAYDVPI 300 PGYKTKTTIN LRLWSTKAPS ADFDLSSYNS GKHTEAAQAL FNAEKICYVL YPGDESNEGK 360 ALRLKQQYTL CSASLQDIIA RFETRSGGRV NWEEFPEKVA VQMNDTHPTL CIPELMRILM 420 DLKGLSWEDA WKITQRTVAY TNHTVLPEAL EKWSLDLMGK LLPRHVEIIE MIDEELVSTI 480 VSEYGTEDPD LLKEKLKAMR ILENVELPSA FADVIVKPKK KTVTAKNTEE SKEDAQTVVK 540 KEQEEEVEVI PEPTVKLPKM VRMANLAVVG GHAVNGVAEI HSEIVKKDVF NEFVKLWPDK 600 FQNKTNGVTP RRWIRFCNPY LSDIITNWIG TEDWVLNTEK LAELRKFADD EALQSEWRAA 660 KKKNKLKVVS LIKERTGYTV NPDAMFDIQI KRIHEYKRQL LNILGIVYRY KKMKEMSASE 720 REKAFVPRVC IFGGKAFATY LQAKRIVKFI TDVGSTINND PEIGDLLKVI FVPDYNVSVA 780 ELLIPASELS QHISTAGMEA SGTSNMKFSM NGCILIGTLD GANVEIREEV GEENFFLFGA 840 KADEIVNLRK ERAEGKFVPD PIFEEVKQYV RSGVFGSNYD ELIGSLEGNE GFGRADYFLV 900 GKDFPSYVEC QEKVDEAYRD QKRWTRMSIL NTAGSFKFSS DRTIHEYAKD IWNIKQVELP 960 * 1020 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PRK14985 | PRK14985 | 7.0e-133 | 559 | 955 | 411 | + maltodextrin phosphorylase; Provisional | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 97 | 954 | 868 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| TIGR02093 | P_ylase | 0 | 100 | 954 | 864 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| pfam00343 | Phosphorylase | 0 | 180 | 955 | 782 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| COG0058 | GlgP | 0 | 111 | 955 | 851 | + Glucan phosphorylase [Carbohydrate transport and metabolism] | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACJ11757.1 | 0 | 59 | 960 | 5 | 935 | alpha-1,4 glucan phosphorylase [Gossypium hirsutum] |
| RefSeq | NP_189578.1 | 0 | 1 | 960 | 1 | 962 | glucan phosphorylase, putative [Arabidopsis thaliana] |
| RefSeq | XP_002279075.1 | 0 | 57 | 960 | 55 | 958 | PREDICTED: similar to Alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic [Vitis vinifera] |
| RefSeq | XP_002305367.1 | 0 | 49 | 960 | 5 | 949 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002526085.1 | 0 | 11 | 960 | 18 | 977 | glycogen phosphorylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1z8d_A | 0 | 78 | 955 | 6 | 829 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 2ati_B | 0 | 92 | 959 | 23 | 832 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 2ati_A | 0 | 92 | 959 | 23 | 832 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 1fc0_B | 0 | 92 | 959 | 23 | 832 | A Chain A, Human Liver Glycogen Phosphorylase Complexed With N-Acetyl-Beta-D- Glucopyranosylamine |
| PDB | 1fc0_A | 0 | 92 | 959 | 23 | 832 | A Chain A, Human Liver Glycogen Phosphorylase Complexed With N-Acetyl-Beta-D- Glucopyranosylamine |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1826 | EC-2.4.1.1 | phosphorylase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO778303 | 864 | 120 | 961 | 0 |
| HO797178 | 384 | 579 | 961 | 0 |
| HO613954 | 506 | 457 | 961 | 0 |
| HO418036 | 575 | 306 | 877 | 0 |
| HO778303 | 121 | 3 | 122 | 9e-16 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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