| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra026011 |
| Family | GT20 |
| Protein Properties | Length: 845 Molecular Weight: 95666 Isoelectric Point: 6.4496 |
| Chromosome | Chromosome/Scaffold: 06 Start: 6525517 End: 6529562 |
| Description | trehalose-phosphatase/synthase 2 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 5 | 478 | 0 |
| DESCNKRQRLLVVANRLPVSAKRTGENSWSLEMSPGGLISGLLGVAAEFETKWVGWPGVDVYDVVGKNALSKSLADMKCIPVFLEEVFDQYYNGYCNGIL WPILHHMGLPQEDHNDTNKTYQTQYDAYKKANRKFLDVIIENYEEGDIVWIHDYHLLFLPQYLKEYNNKIKIGWFLHSPFPSSEVFKTLPSRSELLRSVL TADLLGFHTYDFASHFVRTCTRILGVEGTHEGIVDHGKVTRVAVFPIGIDPNRFLKACELPEVRQQMTELKQRFAGKKVILGVDRLDMIKGIPQKFLGFE KFLEENMDWRDKVVLVQIAVPTRNNVPEYQKLKSQVHGLAGRINGRFGSVSSLPIHHLDCSVDFNFLCALYAIADVMLVTSLRDGMNLVSYEFIACQEAK KGVLVLSEFAGAAQSLGAGAILVNPWDVTEVSSAIKEALNMSAEERDERHRLNFQYVKTHSAKKWGDDFISELH | |||
| Full Sequence |
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| Protein Sequence Length: 845 Download |
| MVSYDESCNK RQRLLVVANR LPVSAKRTGE NSWSLEMSPG GLISGLLGVA AEFETKWVGW 60 PGVDVYDVVG KNALSKSLAD MKCIPVFLEE VFDQYYNGYC NGILWPILHH MGLPQEDHND 120 TNKTYQTQYD AYKKANRKFL DVIIENYEEG DIVWIHDYHL LFLPQYLKEY NNKIKIGWFL 180 HSPFPSSEVF KTLPSRSELL RSVLTADLLG FHTYDFASHF VRTCTRILGV EGTHEGIVDH 240 GKVTRVAVFP IGIDPNRFLK ACELPEVRQQ MTELKQRFAG KKVILGVDRL DMIKGIPQKF 300 LGFEKFLEEN MDWRDKVVLV QIAVPTRNNV PEYQKLKSQV HGLAGRINGR FGSVSSLPIH 360 HLDCSVDFNF LCALYAIADV MLVTSLRDGM NLVSYEFIAC QEAKKGVLVL SEFAGAAQSL 420 GAGAILVNPW DVTEVSSAIK EALNMSAEER DERHRLNFQY VKTHSAKKWG DDFISELHDT 480 FSVSDMKIKK IPLELPQQDV IQRYSKSNNR LIILGFFGTL TEPMKNQNEE LDLKLNPELK 540 RTLKALCSDP KTTVVVLSRS GKNILDKIFG EYNIWLAAEN GMFLRDTIGE WVTNIPENMD 600 LDWVDGTKNV FKYFTARTPR SFFEASETSL VWNFENADVE FGRAQARDLL QYLWAGPISN 660 ASVDVVRGNH SVEVHAVGET KGVAVGRILE EIVRKKSMTT PVDYVFCSGY FLEKDEDIYT 720 FFKPEVVSSK LSHETRSKSS TSNHSIKKKK HLSSNVLDLE KGNYFSVAIG QAHTKARYVV 780 DSSHDVMDLL HKLAVADTTT ATTSDSFSET EDYQTRNANA DWKPWINYVK IRELAVGDTE 840 PVDI* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03063 | PLN03063 | 0 | 4 | 795 | 792 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03064 | PLN03064 | 0 | 11 | 794 | 836 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PRK14501 | PRK14501 | 0 | 13 | 794 | 794 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| pfam00982 | Glyco_transf_20 | 0 | 12 | 479 | 475 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| cd03788 | GT1_TPS | 0 | 13 | 478 | 469 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAD50035.1 | 0 | 57 | 803 | 3 | 694 | AC007651_30 Very similar to trehalose-6-phosphate synthase [Arabidopsis thaliana] |
| RefSeq | NP_173142.1 | 0 | 2 | 839 | 1 | 816 | ATTPS2; alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | NP_173143.4 | 0 | 2 | 802 | 1 | 777 | ATTPS3; alpha,alpha-trehalose-phosphate synthase (UDP-forming) [Arabidopsis thaliana] |
| Swiss-Prot | Q9FZ57 | 0 | 1 | 839 | 1 | 817 | TPS2_ARATH RecName: Full=Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 2; AltName: Full=Trehalose-6-phosphate synthase 2; Short=AtTPS2 |
| Swiss-Prot | Q9SHG0 | 0 | 2 | 802 | 1 | 724 | TPS3_ARATH RecName: Full=Probable alpha,alpha-trehalose-phosphate synthase [UDP-forming] 3; AltName: Full=Trehalose-6-phosphate synthase 3; Short=AtTPS3 |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 13 | 477 | 3 | 451 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 13 | 477 | 3 | 451 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 13 | 477 | 3 | 451 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 13 | 477 | 3 | 451 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1gz5_D | 0 | 13 | 477 | 2 | 450 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| ES813375 | 361 | 374 | 728 | 0 |
| DW069910 | 288 | 99 | 386 | 0 |
| CB629539 | 271 | 191 | 461 | 0 |
| ES332740 | 306 | 127 | 431 | 0 |
| CV711307 | 294 | 11 | 303 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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