| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra026850 |
| Family | AA3 |
| Protein Properties | Length: 515 Molecular Weight: 56727.3 Isoelectric Point: 7.2635 |
| Chromosome | Chromosome/Scaffold: 09 Start: 33982935 End: 33984479 |
| Description | Glucose-methanol-choline (GMC) oxidoreductase family protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA3 | 23 | 498 | 0 |
| SGKSFDYIVVGGGTAGCSLAATLSEKFSVLVIERGGSPFGDPLVEEKKYFGYSLLKTDEYSSVAQSFTSRDGVENYRGRVLGGSSAINGGFYSRASEEFV KKAGWDKDMVQESYKWVESKVVFMPELTQWQSVVQFGFLEAGFYPYNGYNLEHTQGTKIGGSIYDQCGKRHTSADLLGFGRPNHITVLLNATVKSVIFDG SNTRAVGVRFMKSDGSSSKSYKVHVEKRRGEVILTAGALGSPQILLLSGIGPEDHLNDLNIPVVVNLRDVGRRMSDNPAISLLVDRFSQNRTLEPPQVAA IAEGYKYILESEVLPTSITTTRISIAAKIAFPKSRGRLKLNSTNPRENPSVKFNYLGSKEDLEACLEMVLLLQHVARSETVTFYLGLQNREKLLAGDEEL KSFCKDNVRTYYHYHGGCVVGSVVDEDYRVSGVRRLRVVDGSTFEESPGTNPMATVLMLGRYQGIKMLKEREAEKE | |||
| Full Sequence |
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| Protein Sequence Length: 515 Download |
| MLINLSHGAM QMPYMTSDPR EVSGKSFDYI VVGGGTAGCS LAATLSEKFS VLVIERGGSP 60 FGDPLVEEKK YFGYSLLKTD EYSSVAQSFT SRDGVENYRG RVLGGSSAIN GGFYSRASEE 120 FVKKAGWDKD MVQESYKWVE SKVVFMPELT QWQSVVQFGF LEAGFYPYNG YNLEHTQGTK 180 IGGSIYDQCG KRHTSADLLG FGRPNHITVL LNATVKSVIF DGSNTRAVGV RFMKSDGSSS 240 KSYKVHVEKR RGEVILTAGA LGSPQILLLS GIGPEDHLND LNIPVVVNLR DVGRRMSDNP 300 AISLLVDRFS QNRTLEPPQV AAIAEGYKYI LESEVLPTSI TTTRISIAAK IAFPKSRGRL 360 KLNSTNPREN PSVKFNYLGS KEDLEACLEM VLLLQHVARS ETVTFYLGLQ NREKLLAGDE 420 ELKSFCKDNV RTYYHYHGGC VVGSVVDEDY RVSGVRRLRV VDGSTFEESP GTNPMATVLM 480 LGRYQGIKML KEREAEKEGD ESFLSPQGSP QPQP* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01810 | betA | 6.0e-29 | 28 | 299 | 312 | + choline dehydrogenase. Choline dehydrogenase catalyzes the conversion of exogenously supplied choline into the intermediate glycine betaine aldehyde, as part of a two-step oxidative reaction leading to the formation of osmoprotectant betaine. This enzymatic system can be found in both gram-positive and gram-negative bacteria. As in Escherichia coli , Staphylococcus xylosus , and Sinorhizobium meliloti, this enzyme is found associated in a transciptionally co-induced gene cluster with betaine aldehyde dehydrogenase, the second catalytic enzyme in this reaction. Other gram-positive organisms have been shown to employ a different enzymatic system, utlizing a soluable choline oxidase or type III alcohol dehydrogenase instead of choline dehydrogenase. This enzyme is a member of the GMC oxidoreductase family (pfam00732 and pfam05199), sharing a common evoluntionary origin and enzymatic reaction with alcohol dehydrogenase. Outgrouping from this model, Caulobacter crescentus shares sequence homology with choline dehydrogenase, yet other genes participating in this enzymatic reaction have not currently been identified [Cellular processes, Adaptations to atypical conditions]. | ||
| pfam05199 | GMC_oxred_C | 1.0e-31 | 354 | 482 | 136 | + GMC oxidoreductase. This domain found associated with pfam00732. | ||
| TIGR03970 | Rv0697 | 1.0e-43 | 28 | 482 | 500 | + dehydrogenase, Rv0697 family. This model describes a set of dehydrogenases belonging to the glucose-methanol-choline oxidoreductase (GMC oxidoreductase) family. Members of the present family are restricted to Actinobacterial genome contexts containing also members of families TIGR03962 and TIGR03969 (the mycofactocin system), and are proposed to be uniform in function. | ||
| COG2303 | BetA | 2.0e-54 | 23 | 483 | 540 | + Choline dehydrogenase and related flavoproteins [Amino acid transport and metabolism] | ||
| PLN02785 | PLN02785 | 2.0e-162 | 6 | 499 | 557 | + Protein HOTHEAD | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016614 | oxidoreductase activity, acting on CH-OH group of donors |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_172871.1 | 0 | 12 | 514 | 1 | 501 | glucose-methanol-choline (GMC) oxidoreductase family protein [Arabidopsis thaliana] |
| RefSeq | NP_563939.1 | 0 | 1 | 493 | 12 | 503 | glucose-methanol-choline (GMC) oxidoreductase family protein [Arabidopsis thaliana] |
| RefSeq | XP_002312249.1 | 0 | 15 | 492 | 1 | 486 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002315047.1 | 0 | 11 | 492 | 3 | 489 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002522448.1 | 0 | 1 | 494 | 19 | 526 | glucose-methanol-choline (gmc) oxidoreductase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3gdp_B | 0 | 26 | 495 | 26 | 519 | A Chain A, Solution Structure Of The C-Terminal Domain Ole E 9 |
| PDB | 3gdp_A | 0 | 26 | 495 | 26 | 519 | A Chain A, Solution Structure Of The C-Terminal Domain Ole E 9 |
| PDB | 3gdn_B | 0 | 26 | 495 | 26 | 519 | A Chain A, Almond Hydroxynitrile Lyase In Complex With Benzaldehyde |
| PDB | 3gdn_A | 0 | 26 | 495 | 26 | 519 | A Chain A, Almond Hydroxynitrile Lyase In Complex With Benzaldehyde |
| PDB | 1ju2_B | 0 | 26 | 495 | 26 | 519 | A Chain A, Crystal Structure Of The Hydroxynitrile Lyase From Almond |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| amygdalin and prunasin degradation | MANDELONITRILE-LYASE-RXN | EC-4.1.2.10 | (R)-mandelonitrile lyase |
| vicianin bioactivation | MANDELONITRILE-LYASE-RXN | EC-4.1.2.10 | (R)-mandelonitrile lyase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EX098969 | 279 | 168 | 446 | 0 |
| HO796743 | 535 | 28 | 499 | 0 |
| JZ191231 | 268 | 21 | 278 | 0 |
| JZ194700 | 272 | 12 | 273 | 0 |
| JZ191665 | 243 | 12 | 249 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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