| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra026984 |
| Family | GH32 |
| Protein Properties | Length: 691 Molecular Weight: 76862.5 Isoelectric Point: 6.409 |
| Chromosome | Chromosome/Scaffold: 09 Start: 34617520 End: 34622202 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 164 | 471 | 0 |
| GPLFYKGWYHFFYQYNPNAAVWGDIVWGHAVSKDLIHWVHLPIAMVADQWYDANGVWTGSATFLDDGSLVMLYTGSTDKSVQVQNLAYPEDLNDPLLLKW VKYSGNPVLVPPPGILPKDFRDPTTAWKTSDGKWRITIGSKINKTGISLVYDTIDFKTYEKHDTLLHKVPNTGMWECVDFYPVSKTAINGLDTSVNGPNV KHIVKASMDDTRFDHYAVGTYFDSNGTWIPDDPTIDVGMSASLRYDYGKFYASKTFYDQNKGRRILWSWIGESDSEAADVQKGWSSLQGIPRTVVLDTKT GKNLVQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 691 Download |
| MASSDALLPI SAREEDPLVS DGSRSDPNAE THRRKRPVKG LLAVSFGLLF IAFYVVLIAT 60 HHGSSRSNDV KIESDETATT TSRARLAGVS EKSNDQLWKL SGERNTVSFA WNNSVLSWQR 120 TAFHFQPEQN WMNDPNGPEP KRLVADQRSD RREPAETRRR PDPGPLFYKG WYHFFYQYNP 180 NAAVWGDIVW GHAVSKDLIH WVHLPIAMVA DQWYDANGVW TGSATFLDDG SLVMLYTGST 240 DKSVQVQNLA YPEDLNDPLL LKWVKYSGNP VLVPPPGILP KDFRDPTTAW KTSDGKWRIT 300 IGSKINKTGI SLVYDTIDFK TYEKHDTLLH KVPNTGMWEC VDFYPVSKTA INGLDTSVNG 360 PNVKHIVKAS MDDTRFDHYA VGTYFDSNGT WIPDDPTIDV GMSASLRYDY GKFYASKTFY 420 DQNKGRRILW SWIGESDSEA ADVQKGWSSL QGIPRTVVLD TKTGKNLVQW PVEEIKSLRL 480 SSKQFDMEVG PGSVVPVDVN TATQLDIEAE FEIKKETLDK ILGDSSVVAE AEEFSCQKSG 540 GSTVRGALGP FGFSVLADKS LSEQTPVYFY VAKGKDSKLK AFFCTDTSRS TFANDVVKPI 600 YGSSVPVLEG EKLTMRILVD HSIVEGFAQG GRTCITSRVY PTKAIYGAAK LFVFNNAIDA 660 TVTASFKVWQ MNSAFIHPYS EEAVRALSST * 720 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 7.0e-53 | 120 | 641 | 540 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 4.0e-69 | 120 | 670 | 567 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 2.0e-90 | 130 | 474 | 351 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 3.0e-148 | 124 | 471 | 356 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 0 | 124 | 631 | 518 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAG36942.1 | 0 | 1 | 690 | 1 | 663 | AF274298_1 acid invertase [Brassica oleracea] |
| GenBank | AAG36943.1 | 0 | 1 | 690 | 1 | 662 | AF274299_1 acid invertase [Brassica oleracea] |
| EMBL | CAA66330.1 | 0 | 5 | 690 | 1 | 660 | beta-fructosidase [Arabidopsis thaliana] |
| RefSeq | NP_563901.1 | 0 | 1 | 690 | 1 | 664 | ATBETAFRUCT4; beta-fructofuranosidase/ hydrolase, hydrolyzing O-glycosyl compounds [Arabidopsis thaliana] |
| RefSeq | NP_564798.1 | 0 | 1 | 690 | 1 | 648 | beta-fructosidase (BFRUCT3) / beta-fructofuranosidase / invertase, vacuolar [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 105 | 679 | 4 | 544 | A Chain A, Higher-density Crystal Structure Of Potato Endo-1,3-beta-glucanase |
| PDB | 3ugh_A | 0 | 105 | 679 | 4 | 544 | A Chain A, Higher-density Crystal Structure Of Potato Endo-1,3-beta-glucanase |
| PDB | 3ugg_B | 0 | 105 | 679 | 4 | 544 | A Chain A, Higher-density Crystal Structure Of Potato Endo-1,3-beta-glucanase |
| PDB | 3ugg_A | 0 | 105 | 679 | 4 | 544 | A Chain A, Higher-density Crystal Structure Of Potato Endo-1,3-beta-glucanase |
| PDB | 3ugf_B | 0 | 105 | 679 | 4 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| fructan biosynthesis | RXN-1781 | EC-2.4.1.99 | sucrose:sucrose fructosyltransferase |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
