| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra028332 |
| Family | GH31 |
| Protein Properties | Length: 990 Molecular Weight: 110285 Isoelectric Point: 5.2191 |
| Chromosome | Chromosome/Scaffold: 01 Start: 18447727 End: 18454453 |
| Description | heteroglycan glucosidase 1 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH31 | 174 | 585 | 0 |
| STPTAVLQSLSHAVGTVFMPPKWALGYHQCRWSYMSGKRVAEIAQTFRDKKIPSDVIWMDIDYMDGFRCFTFDKERFPDPSALAKHLHNNGFKAIWMLDP GIKQEEGFDVYDSGSKNNVWVKGADGEPFIGEVWPGPCVFPDYTNSKARSWWANLVKDFISNGVDGIWNDMNEPAVFKVVTKTMPENNIHRGDDELGGVQ YHSHYHNVYGMLMARSTYEGMELADKNKRPFVLTRAGFIGSQRYAATWTGDNLSTWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVG AMFPFCRGHSEAGTDDHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYIAHTTGAPVATPIFFADPKDSRLRTIENAFLLGSLLIQASTLSNQGSHELQ PIMPRGTWLRFD | |||
| Full Sequence |
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| Protein Sequence Length: 990 Download |
| MSVSGGDGSE TAPSDMIFGP ILEDGVFRFD CSVEHRKAAF PSVSFKNSKD REVPIPSHIV 60 PAFTPTYSLL EEQQVVTFQF SPGTSFYGTG EVGGQLERTG KRVFTWNTDA WGYGPGTTSL 120 YQSHPWVLVV LPTGETLGVL ADTTRKCEID LRKEGVIRII SPPSYPIITF GPFSTPTAVL 180 QSLSHAVGTV FMPPKWALGY HQCRWSYMSG KRVAEIAQTF RDKKIPSDVI WMDIDYMDGF 240 RCFTFDKERF PDPSALAKHL HNNGFKAIWM LDPGIKQEEG FDVYDSGSKN NVWVKGADGE 300 PFIGEVWPGP CVFPDYTNSK ARSWWANLVK DFISNGVDGI WNDMNEPAVF KVVTKTMPEN 360 NIHRGDDELG GVQYHSHYHN VYGMLMARST YEGMELADKN KRPFVLTRAG FIGSQRYAAT 420 WTGDNLSTWE HLHMSISMVL QLGLSGQPLS GPDIGGFAGN ATPRLFGRWM GVGAMFPFCR 480 GHSEAGTDDH EPWSFGEECE EVCRAALKRR YQLLPHFYTL FYIAHTTGAP VATPIFFADP 540 KDSRLRTIEN AFLLGSLLIQ ASTLSNQGSH ELQPIMPRGT WLRFDFEDSH PDLPTLYLQG 600 GSIIALGPPH LHVGESSLSD DLTLLVSLDE NGKAVGLLFE DDGDGYGYTE GRYLITHYIA 660 ERQSSVVTVK ILKTEGEWER PKRRIHVQLL LGGGAMLDAW GMDGETIQIK MPSESEVSAL 720 ISTSNERFAL HMENTKLIPE KEVLHGQKGM ELSREPVELN SGDWKLNIVP WIGGRILSMT 780 HVPSGVQWLH SRIEMNGYEE YSGTEYRSAG CTEEYNVIER DLEQAGEDES LILEGDVGGG 840 LVLRRKIAIP KDNPRVVQIA SSIEARSVGA GSGGFSRLVC LRVHPTFCLM HPTESFVSFT 900 SIDGTKHEVW PDSGEQLYEG NNLPHGEWML VDKSLNLGLV NRFDVSQVFK CVVHWDCGTV 960 NLELWSEDRP VSKESPLKIE HEYEVASFS* 1020 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd06603 | GH31_GANC_GANAB_alpha | 5.0e-132 | 188 | 528 | 345 | + This family includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae. | ||
| COG1501 | COG1501 | 7.0e-133 | 62 | 615 | 576 | + Alpha-glucosidases, family 31 of glycosyl hydrolases [Carbohydrate transport and metabolism] | ||
| pfam01055 | Glyco_hydro_31 | 4.0e-167 | 175 | 604 | 448 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
| PLN02763 | PLN02763 | 0 | 15 | 985 | 971 | + hydrolase, hydrolyzing O-glycosyl compounds | ||
| cd06604 | GH31_glucosidase_II_MalA | 0 | 188 | 528 | 342 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAB02784.1 | 0 | 14 | 988 | 4 | 958 | alpha glucosidase-like protein [Arabidopsis thaliana] |
| EMBL | CBI37476.1 | 0 | 15 | 985 | 83 | 1053 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_566736.1 | 0 | 6 | 988 | 5 | 990 | HGL1 (heteroglycan glucosidase 1); hydrolase, hydrolyzing O-glycosyl compounds [Arabidopsis thaliana] |
| RefSeq | XP_002263148.1 | 0 | 16 | 985 | 1 | 969 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002519886.1 | 0 | 9 | 985 | 11 | 987 | neutral alpha-glucosidase ab precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3w38_A | 0 | 54 | 707 | 178 | 848 | A Chain A, Endocellulase E1 From Acidothermus Cellulolyticus Mutant Y245g |
| PDB | 3w37_A | 0 | 54 | 707 | 178 | 848 | A Chain A, Endocellulase E1 From Acidothermus Cellulolyticus Mutant Y245g |
| PDB | 3ctt_A | 0 | 82 | 644 | 173 | 774 | A Chain A, Endocellulase E1 From Acidothermus Cellulolyticus Mutant Y245g |
| PDB | 2qmj_A | 0 | 82 | 644 | 173 | 774 | A Chain A, Endocellulase E1 From Acidothermus Cellulolyticus Mutant Y245g |
| PDB | 2qly_A | 0 | 82 | 644 | 173 | 774 | A Chain A, Crystral Structure Of The N-Terminal Subunit Of Human Maltase- Glucoamylase |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-2141 | EC-3.2.1.20 | α-glucosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO779383 | 644 | 343 | 985 | 0 |
| EG431953 | 280 | 322 | 601 | 0 |
| ES901722 | 269 | 271 | 539 | 0 |
| HO783906 | 313 | 225 | 537 | 0 |
| HO783906 | 77 | 471 | 547 | 0.0005 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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