| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra028787 |
| Family | GT64 |
| Protein Properties | Length: 718 Molecular Weight: 81484.5 Isoelectric Point: 8.9109 |
| Chromosome | Chromosome/Scaffold: 02 Start: 2061872 End: 2064537 |
| Description | glycosyltransferase family protein 47 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT64 | 471 | 706 | 0 |
| FTLATMTYDARLWNLKMYVKHYSRCPSVKEILVIWNKGQPPELTELDSAVPVRIRVEKLNSLNNRFNIDPLIKTRAVLELDDDIMMPCDTIEKGFRVWRE YPERLVGYYPRFVDETMSYSAEKFARSHKGYNMILTGAAFMDVGFAFGLYQSEKARLGREFVDEQFNCEDVLLNFLYANVSGLGKAVEYVRPSFAIDTSK FSGVAISGNTNDHYRKRSKCLRRFSDLYGSLSDRRW | |||
| Full Sequence |
|---|
| Protein Sequence Length: 718 Download |
| MRRFLFFVSC FGLFALIAIT YAWLAFPPHI GRTDHVSSSS LGCREDNEGS WSIGVFYGDS 60 PFTLKPIETI NVWRNESAAW PVANPVLTCV SLTNYGFPSN FVAGPFLYVQ GDTLYMFFEN 120 KNPITMQGDI GVAQSTDKGA TWKPLGIALD EPWHLSFPFV FNYNEQIYMM PESNQIGELR 180 LYRAVNFPLT WKLEKVILQK PLVDSTILNH QGNYWLFGSD HSSFGHLEIW YSATPLGPWK 240 PHKKNPIHNG ARNGGRAFLH KGNLYRVGQA SSESYEKKIR IYKIEILSKE EYREVQVPFD 300 LETSHKGQNS WNGVRQHRFD VLQLSSGEYI GLVDGDRVTS GDLVFRVFLG SASLAGAITL 360 VVLLGFFLGI LNCIVPSTWC VKYYKGKRTD AVLNLETTSF VSEQLRRMCS RLNRVPPFLR 420 GLVKPNSTFG RLTLGLLLVL GALLTCVGIS YIYGGSGAVL PYTLRGHVSQ FTLATMTYDA 480 RLWNLKMYVK HYSRCPSVKE ILVIWNKGQP PELTELDSAV PVRIRVEKLN SLNNRFNIDP 540 LIKTRAVLEL DDDIMMPCDT IEKGFRVWRE YPERLVGYYP RFVDETMSYS AEKFARSHKG 600 YNMILTGAAF MDVGFAFGLY QSEKARLGRE FVDEQFNCED VLLNFLYANV SGLGKAVEYV 660 RPSFAIDTSK FSGVAISGNT NDHYRKRSKC LRRFSDLYGS LSDRRWEFGG RKDGWDL* 720 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd08978 | GH_F | 0.0001 | 70 | 260 | 210 | + Glycosyl hydrolase families 43 and 62 form CAZY clan GH-F. This glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) includes family 43 (GH43) and 62 (GH62). GH43 includes enzymes with beta-xylosidase (EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanases (beta-xylanases) and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. GH62 includes enzymes characterized as arabinofuranosidases (alpha-L-arabinofuranosidases; EC 3.2.1.55) that specifically cleave either alpha-1,2 or alpha-1,3-L-arabinofuranose side chains from xylans. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. GH62 are also predicted to be inverting enzymes. A common structural feature of both, GH43 and GH62 enzymes, is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam09258 | Glyco_transf_64 | 2.0e-78 | 471 | 709 | 248 | + Glycosyl transferase family 64 domain. Members of this family catalyze the transfer reaction of N-acetylglucosamine and N-acetylgalactosamine from the respective UDP-sugars to the non-reducing end of [glucuronic acid]beta 1-3[galactose]beta 1-O-naphthalenemethanol, an acceptor substrate analog of the natural common linker of various glycosylaminoglycans. They are also required for the biosynthesis of heparan-sulphate. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016758 | transferase activity, transferring hexosyl groups |
| GO:0031227 | intrinsic to endoplasmic reticulum membrane |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAB85556.1 | 0 | 2 | 717 | 38 | 764 | putative protein [Arabidopsis thaliana] |
| RefSeq | NP_196070.2 | 0 | 2 | 717 | 39 | 765 | glycosyltransferase family protein 47 [Arabidopsis thaliana] |
| RefSeq | XP_002262646.1 | 0 | 39 | 717 | 69 | 756 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002315348.1 | 0 | 28 | 717 | 86 | 847 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002532924.1 | 0 | 39 | 717 | 17 | 704 | transferase, transferring glycosyl groups, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1on8_B | 8e-25 | 465 | 701 | 24 | 270 | A Chain A, Native Cytokinin Dehydrogenase |
| PDB | 1on8_A | 8e-25 | 465 | 701 | 24 | 270 | A Chain A, Native Cytokinin Dehydrogenase |
| PDB | 1on6_B | 8e-25 | 465 | 701 | 24 | 270 | A Chain A, Native Cytokinin Dehydrogenase |
| PDB | 1on6_A | 8e-25 | 465 | 701 | 24 | 270 | A Chain A, Native Cytokinin Dehydrogenase |
| PDB | 1omz_B | 8e-25 | 465 | 701 | 24 | 270 | A Chain A, Native Cytokinin Dehydrogenase |
