| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra029152 |
| Family | AA3 |
| Protein Properties | Length: 533 Molecular Weight: 58649.5 Isoelectric Point: 5.3458 |
| Chromosome | Chromosome/Scaffold: 03 Start: 6583595 End: 6585446 |
| Description | Glucose-methanol-choline (GMC) oxidoreductase family protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA3 | 11 | 526 | 0 |
| ARFDYIVIGGGTSGCSLAATLSQNASVLVLERGGSPYDNPRATDIENVANTLLNITPNSWSQPFISEDGVYNTRAGVLGGDSVLKAGFYSRAEEYYVKEA EWEMEEVEAAYEWVERKLVFEPQVTGWQSALKDGLLEAGVLPYNGFTFKHIIGTKIGGSTFDSAGHKHSAADLLEYANPDKIAVYLHATVHKILFTTKGN QRPKAYGMIYQDADGMFHKVELAENAMNEVILSAGALGSPQLLMLSGVGPRAHLEAQGVDPVVIDHPMVGQGMGDNPMNSVIVPSPQPVELSLPQVVGIT RFGNFIEGFSGLSLSYNLTRMFFETRLSTQSITSFINSSDFQLNLIEIDGVIFQKVDGPFSRGYLELRNTNPDDNPSVTFNYYQEPEDLEKCVKGLETII EVINSNAFSKYKYLNATGRELLNRMLGLPTNLRPRHVTSVFNLRQFCIDTVMSVWHYHGGCQVGRVVDKDYKVLGIDALRVIDGSTFLKSPGTNPQATVM MLGRYMGQKILRERNA | |||
| Full Sequence |
|---|
| Protein Sequence Length: 533 Download |
| MKDATSAPTY ARFDYIVIGG GTSGCSLAAT LSQNASVLVL ERGGSPYDNP RATDIENVAN 60 TLLNITPNSW SQPFISEDGV YNTRAGVLGG DSVLKAGFYS RAEEYYVKEA EWEMEEVEAA 120 YEWVERKLVF EPQVTGWQSA LKDGLLEAGV LPYNGFTFKH IIGTKIGGST FDSAGHKHSA 180 ADLLEYANPD KIAVYLHATV HKILFTTKGN QRPKAYGMIY QDADGMFHKV ELAENAMNEV 240 ILSAGALGSP QLLMLSGVGP RAHLEAQGVD PVVIDHPMVG QGMGDNPMNS VIVPSPQPVE 300 LSLPQVVGIT RFGNFIEGFS GLSLSYNLTR MFFETRLSTQ SITSFINSSD FQLNLIEIDG 360 VIFQKVDGPF SRGYLELRNT NPDDNPSVTF NYYQEPEDLE KCVKGLETII EVINSNAFSK 420 YKYLNATGRE LLNRMLGLPT NLRPRHVTSV FNLRQFCIDT VMSVWHYHGG CQVGRVVDKD 480 YKVLGIDALR VIDGSTFLKS PGTNPQATVM MLGRYMGQKI LRERNASGEK RD* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG2303 | BetA | 6.0e-7 | 13 | 101 | 94 | + Choline dehydrogenase and related flavoproteins [Amino acid transport and metabolism] | ||
| TIGR01810 | betA | 2.0e-23 | 197 | 521 | 356 | + choline dehydrogenase. Choline dehydrogenase catalyzes the conversion of exogenously supplied choline into the intermediate glycine betaine aldehyde, as part of a two-step oxidative reaction leading to the formation of osmoprotectant betaine. This enzymatic system can be found in both gram-positive and gram-negative bacteria. As in Escherichia coli , Staphylococcus xylosus , and Sinorhizobium meliloti, this enzyme is found associated in a transciptionally co-induced gene cluster with betaine aldehyde dehydrogenase, the second catalytic enzyme in this reaction. Other gram-positive organisms have been shown to employ a different enzymatic system, utlizing a soluable choline oxidase or type III alcohol dehydrogenase instead of choline dehydrogenase. This enzyme is a member of the GMC oxidoreductase family (pfam00732 and pfam05199), sharing a common evoluntionary origin and enzymatic reaction with alcohol dehydrogenase. Outgrouping from this model, Caulobacter crescentus shares sequence homology with choline dehydrogenase, yet other genes participating in this enzymatic reaction have not currently been identified [Cellular processes, Adaptations to atypical conditions]. | ||
| pfam05199 | GMC_oxred_C | 2.0e-25 | 369 | 513 | 150 | + GMC oxidoreductase. This domain found associated with pfam00732. | ||
| COG2303 | BetA | 6.0e-27 | 234 | 527 | 314 | + Choline dehydrogenase and related flavoproteins [Amino acid transport and metabolism] | ||
| PLN02785 | PLN02785 | 0 | 13 | 528 | 533 | + Protein HOTHEAD | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016614 | oxidoreductase activity, acting on CH-OH group of donors |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAB11043.1 | 0 | 1 | 514 | 34 | 563 | mandelonitrile lyase-like protein [Arabidopsis thaliana] |
| EMBL | CAB87405.1 | 0 | 1 | 514 | 34 | 557 | ADHESION OF CALYX EDGES-like protein [Arabidopsis thaliana] |
| RefSeq | NP_001119417.1 | 0 | 1 | 524 | 1 | 543 | glucose-methanol-choline (GMC) oxidoreductase family protein [Arabidopsis thaliana] |
| RefSeq | NP_200008.1 | 0 | 1 | 524 | 34 | 576 | glucose-methanol-choline (GMC) oxidoreductase family protein [Arabidopsis thaliana] |
| RefSeq | NP_567032.1 | 0 | 1 | 526 | 34 | 569 | glucose-methanol-choline (GMC) oxidoreductase family protein [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1ju2_B | 0 | 3 | 530 | 17 | 523 | A Chain A, Crystal Structure Of The Hydroxynitrile Lyase From Almond |
| PDB | 1ju2_A | 0 | 3 | 530 | 17 | 523 | A Chain A, Crystal Structure Of The Hydroxynitrile Lyase From Almond |
| PDB | 3gdp_B | 0 | 3 | 527 | 17 | 520 | A Chain A, Crystal Structure Of The Hydroxynitrile Lyase From Almond |
| PDB | 3gdp_A | 0 | 3 | 527 | 17 | 520 | A Chain A, Crystal Structure Of The Hydroxynitrile Lyase From Almond |
| PDB | 3gdn_B | 0 | 3 | 527 | 17 | 520 | A Chain A, Almond Hydroxynitrile Lyase In Complex With Benzaldehyde |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cutin biosynthesis | RXN-2121 | EC-1.1.1 | 18-hydroxyoleate dehydrogenase |
| cutin biosynthesis | RXN-9802 | EC-1.1.1 | 16-hydroxypalmitate dehydrogenase |
| suberin biosynthesis | RXN-2121 | EC-1.1.1 | 18-hydroxyoleate dehydrogenase |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO796743 | 527 | 14 | 524 | 0 |
| ES264389 | 220 | 217 | 436 | 0 |
| EX050677 | 249 | 57 | 305 | 0 |
| CX666126 | 290 | 238 | 527 | 0 |
| FG564536 | 204 | 127 | 330 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
