| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra029913 |
| Family | CE10 |
| Protein Properties | Length: 327 Molecular Weight: 35788 Isoelectric Point: 5.242 |
| Chromosome | Chromosome/Scaffold: 01 Start: 14832206 End: 14833186 |
| Description | alpha/beta-Hydrolases superfamily protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE10 | 13 | 320 | 0 |
| LKIYKSGRIERLMGETTVPASLTPQNGVVSKDVVYSPDDNLSVRMYLPVKAAETGEKLPLLVYFHGGGFIIETAFSPTYHTFLTAAVSASDCVAVSVDYR RAPEHPIPISFDDSWTCLKWVFTHIAGSGSESWLNKHVDFSKLFLAGDSAGATIAHHMAMRAAKENLSPELSDSGISGIILVHPYFWSKTPIDDKETKDE TLRSKIEAFWMMAATPNNKDGVDDPLINVVQSEAVDISGLGCGKVLVMVAEKDALARQGWGYAAKLEKSEWKGKVELVESEGEDHVFHLMRPDCEKALEA MKIFAGFL | |||
| Full Sequence |
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| Protein Sequence Length: 327 Download |
| MDSEIAVDCS PLLKIYKSGR IERLMGETTV PASLTPQNGV VSKDVVYSPD DNLSVRMYLP 60 VKAAETGEKL PLLVYFHGGG FIIETAFSPT YHTFLTAAVS ASDCVAVSVD YRRAPEHPIP 120 ISFDDSWTCL KWVFTHIAGS GSESWLNKHV DFSKLFLAGD SAGATIAHHM AMRAAKENLS 180 PELSDSGISG IILVHPYFWS KTPIDDKETK DETLRSKIEA FWMMAATPNN KDGVDDPLIN 240 VVQSEAVDIS GLGCGKVLVM VAEKDALARQ GWGYAAKLEK SEWKGKVELV ESEGEDHVFH 300 LMRPDCEKAL EAMKIFAGFL KGQMKI* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00135 | COesterase | 0.002 | 46 | 179 | 154 | + Carboxylesterase family. | ||
| cd00312 | Esterase_lipase | 0.002 | 51 | 176 | 136 | + Esterases and lipases (includes fungal lipases, cholinesterases, etc.) These enzymes act on carboxylic esters (EC: 3.1.1.-). The catalytic apparatus involves three residues (catalytic triad): a serine, a glutamate or aspartate and a histidine.These catalytic residues are responsible for the nucleophilic attack on the carbonyl carbon atom of the ester bond. In contrast with other alpha/beta hydrolase fold family members, p-nitrobenzyl esterase and acetylcholine esterase have a Glu instead of Asp at the active site carboxylate. | ||
| COG2272 | PnbA | 5.0e-6 | 31 | 176 | 160 | + Carboxylesterase type B [Lipid metabolism] | ||
| COG0657 | Aes | 7.0e-26 | 32 | 321 | 296 | + Esterase/lipase [Lipid metabolism] | ||
| pfam07859 | Abhydrolase_3 | 3.0e-54 | 73 | 301 | 232 | + alpha/beta hydrolase fold. This catalytic domain is found in a very wide range of enzymes. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAL57633.1 | 0 | 1 | 322 | 1 | 322 | AT3g48690/T8P19_200 [Arabidopsis thaliana] |
| DDBJ | BAE98942.1 | 0 | 1 | 322 | 1 | 322 | hypothetical protein [Arabidopsis thaliana] |
| RefSeq | NP_178453.1 | 0 | 1 | 321 | 1 | 311 | hydrolase [Arabidopsis thaliana] |
| RefSeq | NP_190438.1 | 0 | 1 | 322 | 1 | 322 | CXE12; carboxylesterase [Arabidopsis thaliana] |
| RefSeq | NP_190439.1 | 0 | 1 | 322 | 1 | 327 | ATCXE13 (ARABIDOPSIS THALIANA CARBOXYESTERASE 13); hydrolase [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2o7v_A | 7e-32 | 31 | 198 | 47 | 204 | A Chain A, Pectin Methylesterase Pema From Erwinia Chrysanthemi |
| PDB | 2o7r_A | 7e-32 | 31 | 198 | 47 | 204 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 2zsi_A | 8e-32 | 19 | 323 | 46 | 351 | A Chain A, Plant Carboxylesterase Aecxe1 From Actinidia Eriantha With Acyl Adduct |
| PDB | 2zsh_A | 8e-32 | 19 | 323 | 46 | 351 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| PDB | 3ed1_F | 2e-31 | 19 | 324 | 38 | 351 | B Chain B, Structural Basis Of Gibberellin(Ga3)-Induced Della Recognition By The Gibberellin Receptor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| formononetin biosynthesis | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| formononetin biosynthesis | RXN-3625 | - | 2,7-dihydroxy-4'-methoxyisoflavanone dehydratase |
| isoflavonoid biosynthesis I | RXN-3284 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-3303 | EC-4.2.1.105 | 2-hydroxyisoflavanone dehydratase |
| isoflavonoid biosynthesis II | RXN-5502 | - | 2,7,5-trihydroxy-4'-methoxyisoflavanone dehydratase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| FY433054 | 271 | 1 | 271 | 0 |
| EX019947 | 281 | 1 | 281 | 0 |
| EX109246 | 277 | 1 | 277 | 0 |
| EX114931 | 240 | 1 | 240 | 0 |
| EX047810 | 271 | 24 | 294 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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