| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra030750 |
| Family | GT8 |
| Protein Properties | Length: 527 Molecular Weight: 61113.4 Isoelectric Point: 9.5381 |
| Chromosome | Chromosome/Scaffold: 08 Start: 20505459 End: 20507835 |
| Description | plant glycogenin-like starch initiation protein 5 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT8 | 237 | 444 | 9.6e-39 |
| QRVAYVTLLHSSESYVCGAIALAQSIRQSGSRHDMILLHDDSIKNKSRIGLSLAGWKLRRVERISSPFSQKGSYNEWNYSKLRVWQVTDYDKLVFIDADF IIAKNVDYLFFYPQLSAAGNNRVLFNSGVMVLEPSACLFEELMQQSFKIKSYNGGDQGFLNEYFVWWHRLSKRVNTMKYFGEETNIGPKRNLPDNLEGIH YLGIKPWR | |||
| Full Sequence |
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| Protein Sequence Length: 527 Download |
| MITSPSIIEI HYSLPVVPVN RNPRWLRLIK NYLPDQKKIR VGLLNIAENE RESYEATGTS 60 ILENVHVLLD PLPKNLTWES LFPVWIDEDH TWNAPVCPEV PLPQVEGTAA DVDVVVVKTP 120 CDGFSESKGL RDVFRLQVNL AAAKLVVESG RRNVDRTVYV VFIGSCEPMH EIFRCDERVR 180 RVGKYWVYKP NLKKLKQKLL MPVGSCQIAP PVTELDQETW REQKNESLSS TTTFAAQRVA 240 YVTLLHSSES YVCGAIALAQ SIRQSGSRHD MILLHDDSIK NKSRIGLSLA GWKLRRVERI 300 SSPFSQKGSY NEWNYSKLRV WQVTDYDKLV FIDADFIIAK NVDYLFFYPQ LSAAGNNRVL 360 FNSGVMVLEP SACLFEELMQ QSFKIKSYNG GDQGFLNEYF VWWHRLSKRV NTMKYFGEET 420 NIGPKRNLPD NLEGIHYLGI KPWRCYRDYD CNWDLTTRRV YASESVNKKW WKVYDKMPKK 480 LQLYCGLTRK MDKNIEKWMK AAKVKGFPEK HWRVQVRDPR KKNLVD* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG5597 | COG5597 | 8.0e-8 | 229 | 403 | 207 | + Alpha-N-acetylglucosamine transferase [Cell envelope biogenesis, outer membrane] | ||
| PLN00176 | PLN00176 | 3.0e-16 | 222 | 475 | 310 | + galactinol synthase | ||
| cd06914 | GT8_GNT1 | 1.0e-16 | 240 | 400 | 171 | + GNT1 is a fungal enzyme that belongs to the GT 8 family. N-acetylglucosaminyltransferase is a fungal enzyme that catalyzes the addition of N-acetyl-D-glucosamine to mannotetraose side chains by an alpha 1-2 linkage during the synthesis of mannan. The N-acetyl-D-glucosamine moiety in mannan plays a role in the attachment of mannan to asparagine residues in proteins. The mannotetraose and its N-acetyl-D-glucosamine derivative side chains of mannan are the principle immunochemical determinants on the cell surface. N-acetylglucosaminyltransferase is a member of glycosyltransferase family 8, which are, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed, retaining glycosyltransferases. | ||
| pfam01501 | Glyco_transf_8 | 2.0e-36 | 242 | 446 | 248 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd02537 | GT8_Glycogenin | 1.0e-67 | 239 | 477 | 252 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAB70408.1 | 0 | 1 | 523 | 40 | 542 | F7G19.14 [Arabidopsis thaliana] |
| EMBL | CAN81983.1 | 0 | 21 | 526 | 44 | 545 | hypothetical protein [Vitis vinifera] |
| RefSeq | NP_172373.3 | 0 | 1 | 523 | 40 | 562 | PGSIP5 (PLANT GLYCOGENIN-LIKE STARCH INITIATION PROTEIN 5); transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | NP_175891.1 | 0 | 2 | 525 | 37 | 555 | PGSIP4 (PLANT GLYCOGENIN-LIKE STARCH INITIATION PROTEIN 4); transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002280832.1 | 0 | 21 | 526 | 44 | 545 | PREDICTED: hypothetical protein [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1zcv_A | 5e-19 | 240 | 448 | 25 | 245 | A Chain A, Apo Form Of A Mutant Of Glycogenin In Which Asp159 Is Replaced By Asn |
| PDB | 3v8z_A | 9e-19 | 240 | 448 | 25 | 245 | A Chain A, Apo Form Of A Mutant Of Glycogenin In Which Asp159 Is Replaced By Asn |
| PDB | 3v8y_A | 9e-19 | 240 | 448 | 25 | 245 | A Chain A, Structure Of Apo-Glycogenin Truncated At Residue 270 |
| PDB | 1zct_B | 9e-19 | 240 | 448 | 25 | 245 | A Chain A, Structure Of Glycogenin Truncated At Residue 270 In A Complex With Udp |
| PDB | 1zct_A | 9e-19 | 240 | 448 | 25 | 245 | A Chain A, Structure Of Glycogenin Truncated At Residue 270 In A Complex With Udp |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| ES903404 | 278 | 1 | 274 | 0 |
| EV045011 | 246 | 92 | 333 | 0 |
| EL450640 | 331 | 148 | 477 | 0 |
| EL376918 | 273 | 248 | 520 | 0 |
| ES909091 | 182 | 346 | 527 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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