| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra031526 |
| Family | GT20 |
| Protein Properties | Length: 858 Molecular Weight: 97003.1 Isoelectric Point: 6.4374 |
| Chromosome | Chromosome/Scaffold: 01 Start: 16714545 End: 16717320 |
| Description | trehalose phosphate synthase |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 50 | 544 | 0 |
| ISLPCRERKIIVANFLPLNCKKDSETGQWIFSLDNDSPLLHLKDGFSPETEVVYVGSLKTDVDLSEQDEVAQILFEEFSCVPTFLPQDVHKKFYLGFCKQ QLWPLFHYMLPMCPDHGERFDRSLWQAYVSANKIFADKVMGAINLEEDCIWIHDYHLMLLPTFLRRRFYRVKLGFFLHSPFPSSEIYRTLPVREELLRGL LNCDLIGFHTFDYARHFLSCCCRMLGLEYESKRGHIALDYLGRTVFLKILPIGIQMGRLESVLNLPSTAEKLKEIQEKYRGKKVILGVDDMDIFKGLSLK ILAFEHLLQQYPSMLGKVVLIQIVNPARGSGKDVQEAKKETYYTVNRINERYGSPGYEPVVLIDRPVPRFEKSAYYAMAECCIVNAVRDGMNLVPYKYTV CRQGTPEMDKSMGLSDDSPPRTSTLVLSEFIGCSPSLSGAIRVNPWDVDAVADSMYSALTMSDSEKQLRHKKHYQYISTHDVAYWSRSFAQDLER | |||
| Full Sequence |
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| Protein Sequence Length: 858 Download |
| MGSKSFGNLL DLASGDLLDI PHTPRSLPRV MTVPGIIDGY GISDRDSDAI SLPCRERKII 60 VANFLPLNCK KDSETGQWIF SLDNDSPLLH LKDGFSPETE VVYVGSLKTD VDLSEQDEVA 120 QILFEEFSCV PTFLPQDVHK KFYLGFCKQQ LWPLFHYMLP MCPDHGERFD RSLWQAYVSA 180 NKIFADKVMG AINLEEDCIW IHDYHLMLLP TFLRRRFYRV KLGFFLHSPF PSSEIYRTLP 240 VREELLRGLL NCDLIGFHTF DYARHFLSCC CRMLGLEYES KRGHIALDYL GRTVFLKILP 300 IGIQMGRLES VLNLPSTAEK LKEIQEKYRG KKVILGVDDM DIFKGLSLKI LAFEHLLQQY 360 PSMLGKVVLI QIVNPARGSG KDVQEAKKET YYTVNRINER YGSPGYEPVV LIDRPVPRFE 420 KSAYYAMAEC CIVNAVRDGM NLVPYKYTVC RQGTPEMDKS MGLSDDSPPR TSTLVLSEFI 480 GCSPSLSGAI RVNPWDVDAV ADSMYSALTM SDSEKQLRHK KHYQYISTHD VAYWSRSFAQ 540 DLERACRDHY SKRCWGVGWG LGFRLIALSP NFRRLSVEQT VSAYRRSSKR AIFLDYDGTL 600 VPETLIVKEP SADVMSALKA LCSDPNNTVF IVSGRGKVSL SEWLAPCQNL GIAAEHGYFT 660 RWNESCDWET SGFSNDFEWK KIVEPIMRLY TETTDGSTIE AKESALVWHH QDADRDFGSC 720 QAKELLDHLE SVLVNEPVVV NRGHQIVEVK PQGVSKGLVT GKVLRRMLEE GNAPDFVVCI 780 GDDRSDEDMF ESITTTLSAQ PSSEIFACTV GRKPSKAKYF LDEVSDVVKL LQGLANTSSS 840 PKPRYPSHLR VSFESVV* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03063 | PLN03063 | 1.0e-169 | 55 | 843 | 834 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN02205 | PLN02205 | 0 | 1 | 843 | 859 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] | ||
| pfam00982 | Glyco_transf_20 | 0 | 56 | 544 | 493 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PRK14501 | PRK14501 | 0 | 57 | 835 | 788 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| cd03788 | GT1_TPS | 0 | 59 | 543 | 485 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAF01061.1 | 0 | 1 | 857 | 1 | 861 | putative trehalose-6-phosphate synthase [Arabidopsis thaliana] |
| EMBL | CAN78769.1 | 0 | 1 | 857 | 1 | 857 | hypothetical protein [Vitis vinifera] |
| RefSeq | NP_176221.1 | 0 | 1 | 857 | 1 | 861 | ATTPS10 (trehalose phosphate synthase); transferase, transferring glycosyl groups / trehalose-phosphatase [Arabidopsis thaliana] |
| RefSeq | XP_002264873.1 | 0 | 1 | 844 | 1 | 844 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002521023.1 | 0 | 1 | 857 | 1 | 861 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 141 | 544 | 75 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 141 | 544 | 75 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 141 | 544 | 75 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 141 | 544 | 75 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1gz5_D | 0 | 141 | 544 | 74 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO777775 | 767 | 78 | 838 | 0 |
| HO779327 | 516 | 96 | 611 | 0 |
| HO779327 | 255 | 608 | 858 | 0 |
| HO797286 | 480 | 373 | 848 | 0 |
| HO777775 | 78 | 1 | 74 | 0.05 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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