| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra031653 |
| Family | GT8 |
| Protein Properties | Length: 529 Molecular Weight: 61568.8 Isoelectric Point: 9.7981 |
| Chromosome | Chromosome/Scaffold: 09 Start: 35379195 End: 35381490 |
| Description | plant glycogenin-like starch initiation protein 5 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT8 | 241 | 446 | 6.9e-37 |
| VAYATLLHSSEVYVCGAIALAQSIRQSGSTHDMILLHDKSITNPSRVGLSRAGWKLRQVERIRSPFSKKDSYNEWNYSKLRVWQVTDYDKLVFIDADFII AKNVDYLFSFPQLSAAGNDKFLFNSGVMVLEPSACLFKELMLKSFKIKSYNGGDQGFLNEYFVWWHRLSKHVNTMKYFGAESKNSQKRHLPDNLEGIHYL GLKPWL | |||
| Full Sequence |
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| Protein Sequence Length: 529 Download |
| MINSPSIVEI RYSLPTEVNR NPRWFRLIKN HLQEKKIRVG LLNIEENERE SYEATGTSIL 60 ETVHVTLDPL PKNLTWESLF PVWIDEDHRW HTPTCPEVPL PRVEGSDADV DVVVVKVPCD 120 GVREDKGLRD VFRLQVNLAA AKLAVESGRR NVDRTVYVVF IGSCGPMHEI FRCDERVRRV 180 GEYWLYRPNL EKLKQKLVMP VGSCQIAPLP AQLDQEAWRR QKNETLTSRT TLSSSSGAQR 240 VAYATLLHSS EVYVCGAIAL AQSIRQSGST HDMILLHDKS ITNPSRVGLS RAGWKLRQVE 300 RIRSPFSKKD SYNEWNYSKL RVWQVTDYDK LVFIDADFII AKNVDYLFSF PQLSAAGNDK 360 FLFNSGVMVL EPSACLFKEL MLKSFKIKSY NGGDQGFLNE YFVWWHRLSK HVNTMKYFGA 420 ESKNSQKRHL PDNLEGIHYL GLKPWLCYRD YDCNWDLKSR RVYASESVHE KWWKVYDKMP 480 KKLRGYCGLT RKMEKNIEKW RKMAKVKGLP ERHWRIQVRD PRKKNLLD* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00505 | Glyco_transf_8 | 5.0e-9 | 307 | 445 | 171 | + Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+. | ||
| PLN00176 | PLN00176 | 3.0e-10 | 242 | 477 | 290 | + galactinol synthase | ||
| cd06914 | GT8_GNT1 | 4.0e-14 | 241 | 402 | 173 | + GNT1 is a fungal enzyme that belongs to the GT 8 family. N-acetylglucosaminyltransferase is a fungal enzyme that catalyzes the addition of N-acetyl-D-glucosamine to mannotetraose side chains by an alpha 1-2 linkage during the synthesis of mannan. The N-acetyl-D-glucosamine moiety in mannan plays a role in the attachment of mannan to asparagine residues in proteins. The mannotetraose and its N-acetyl-D-glucosamine derivative side chains of mannan are the principle immunochemical determinants on the cell surface. N-acetylglucosaminyltransferase is a member of glycosyltransferase family 8, which are, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed, retaining glycosyltransferases. | ||
| pfam01501 | Glyco_transf_8 | 4.0e-35 | 244 | 448 | 248 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd02537 | GT8_Glycogenin | 3.0e-68 | 241 | 479 | 252 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAB70408.1 | 0 | 1 | 527 | 40 | 544 | F7G19.14 [Arabidopsis thaliana] |
| EMBL | CAN81983.1 | 0 | 12 | 528 | 37 | 545 | hypothetical protein [Vitis vinifera] |
| RefSeq | NP_172373.3 | 0 | 1 | 527 | 40 | 564 | PGSIP5 (PLANT GLYCOGENIN-LIKE STARCH INITIATION PROTEIN 5); transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | NP_175891.1 | 0 | 2 | 527 | 37 | 555 | PGSIP4 (PLANT GLYCOGENIN-LIKE STARCH INITIATION PROTEIN 4); transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002280832.1 | 0 | 12 | 528 | 37 | 545 | PREDICTED: hypothetical protein [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3u2x_B | 2e-18 | 242 | 445 | 6 | 221 | A Chain A, Crystal Structure Of The Putative Tetr-Family Transcriptional Regulator From Rhodococcus Sp. Rha1 |
| PDB | 3u2x_A | 2e-18 | 242 | 445 | 6 | 221 | A Chain A, Crystal Structure Of The Putative Tetr-Family Transcriptional Regulator From Rhodococcus Sp. Rha1 |
| PDB | 3u2v_B | 2e-18 | 242 | 445 | 6 | 221 | A Chain A, Crystal Structure Of The Putative Tetr-Family Transcriptional Regulator From Rhodococcus Sp. Rha1 |
| PDB | 3u2v_A | 2e-18 | 242 | 445 | 6 | 221 | A Chain A, Crystal Structure Of The Putative Tetr-Family Transcriptional Regulator From Rhodococcus Sp. Rha1 |
| PDB | 3u2u_B | 2e-18 | 242 | 445 | 6 | 221 | A Chain A, Crystal Structure Of The Putative Tetr-Family Transcriptional Regulator From Rhodococcus Sp. Rha1 |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| ES903404 | 276 | 1 | 276 | 0 |
| EV045011 | 247 | 89 | 335 | 0 |
| ES909091 | 182 | 348 | 529 | 0 |
| EL450640 | 335 | 146 | 479 | 0 |
| EL376918 | 273 | 250 | 522 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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