| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra031780 |
| Family | AA7 |
| Protein Properties | Length: 539 Molecular Weight: 59948.8 Isoelectric Point: 9.3706 |
| Chromosome | Chromosome/Scaffold: 09 Start: 34772412 End: 34774028 |
| Description | FAD-binding Berberine family protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 67 | 522 | 0 |
| NARFNTTASHKPAIVIVARSEAHVQAAVVCTKTLKFQLKTRSGGHDYEGVSYTSNLPFFLLDMSSFRDITVEGETAWIGAGVTLGEVYYRIWEKTKTHGF PAGVCPTVGAGGHISGGGYGKMIRKYGLSVDYVTDAKIVDVNGRVLDRKGMGEDLFWAINGGGGASFGVILGFKITLVPVPETVTVFRVQKNLDENAVEM VHKWQFVAPKTDPGLFMRVLLQPVTRNKVETVRASVLALFLGKSSEVLSLLNKDFPELGLKQENCTELTWIQSVLWWDNHDNATLVKPEVLLERNLDTAS YGKRKSDFVEKEITKEGLDYLMKKMIEVGKIGLVFNPYGGKVSEVATTKTPFPHRTKLYKVQHSMNWKDPGPEAETSFLEKAKSFYSYMAPFVTKNPRHT YLNYRDLDIGVNSHGPNAYREGEVYGRKYFGENFDRLVKVKSTVDPENFFRDEQSV | |||
| Full Sequence |
|---|
| Protein Sequence Length: 539 Download |
| MKLLCLTFVF LVSTTLTAAT PPDATVYQNF LQCFTTQTRA LPNTLADVVF PQTAAGYTPA 60 LRNYIRNARF NTTASHKPAI VIVARSEAHV QAAVVCTKTL KFQLKTRSGG HDYEGVSYTS 120 NLPFFLLDMS SFRDITVEGE TAWIGAGVTL GEVYYRIWEK TKTHGFPAGV CPTVGAGGHI 180 SGGGYGKMIR KYGLSVDYVT DAKIVDVNGR VLDRKGMGED LFWAINGGGG ASFGVILGFK 240 ITLVPVPETV TVFRVQKNLD ENAVEMVHKW QFVAPKTDPG LFMRVLLQPV TRNKVETVRA 300 SVLALFLGKS SEVLSLLNKD FPELGLKQEN CTELTWIQSV LWWDNHDNAT LVKPEVLLER 360 NLDTASYGKR KSDFVEKEIT KEGLDYLMKK MIEVGKIGLV FNPYGGKVSE VATTKTPFPH 420 RTKLYKVQHS MNWKDPGPEA ETSFLEKAKS FYSYMAPFVT KNPRHTYLNY RDLDIGVNSH 480 GPNAYREGEV YGRKYFGENF DRLVKVKSTV DPENFFRDEQ SVPTLPSKPA NTPAKPAL* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG0277 | GlcD | 2.0e-13 | 59 | 525 | 498 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam08031 | BBE | 7.0e-16 | 466 | 523 | 58 | + Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine. | ||
| pfam01565 | FAD_binding_4 | 7.0e-18 | 78 | 213 | 139 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0016491 | oxidoreductase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAD94511.1 | 0 | 18 | 532 | 11 | 532 | hypothetical protein [Arabidopsis thaliana] |
| RefSeq | NP_171700.1 | 0 | 1 | 532 | 1 | 541 | ATSEC1A; FAD binding / catalytic/ electron carrier/ oxidoreductase [Arabidopsis thaliana] |
| RefSeq | NP_172642.3 | 0 | 1 | 530 | 1 | 534 | FAD binding / catalytic/ electron carrier/ oxidoreductase [Arabidopsis thaliana] |
| RefSeq | NP_193816.1 | 0 | 26 | 528 | 34 | 538 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| RefSeq | NP_974580.1 | 0 | 25 | 528 | 34 | 539 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3vte_A | 0 | 28 | 526 | 5 | 514 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 4dns_B | 0 | 19 | 525 | 1 | 496 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 4dns_A | 0 | 19 | 525 | 1 | 496 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 3tsj_B | 0 | 22 | 528 | 3 | 499 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 3tsj_A | 0 | 22 | 528 | 3 | 499 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cannabinoid biosynthesis | RXN-7854 | EC-1.21.3 | tetrahydrocannabinolic acid synthase |
