| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra033098 |
| Family | GT35 |
| Protein Properties | Length: 934 Molecular Weight: 105250 Isoelectric Point: 5.8241 |
| Chromosome | Chromosome/Scaffold: 02 Start: 21444132 End: 21448923 |
| Description | Glycosyl transferase, family 35 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT35 | 146 | 928 | 0 |
| ALKSLGFELESVAGQEPDPALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQIITKDGQEEAAEDWLELSNPWEIVRNDVSYPIKFYGKVVFGS DGKKRWIGGEDVVAVAYDVPIPGYKTKTTINLRLWSTKAPSSDFDLSSYNSGKHTEAAEALFNAEKICYVLYPGDESDEGKALRLKQQYTLCSASLQDIV ARFETRSGGSVNWEEFPEKVAVQMNDTHPTLCIPELMRILMDLKGLSWEDAWRITQRTVAYTNHTVLPEALEKWSLELMEKLLPRHVEIIEKIDEELVST IVSEYGTENPDLLKEKLKAMRILENVELPSAFADVIVKPESKPAIAEKTVDQTKDSKEDAQTVVKKEVEEEETAGVVAEVIPEPPKMVRMANLAVVGGHA VNGVAEIHSEIVKTDVFNDFVKLWPDKFQNKTNGVTPRRWIRFCNPYLSDIITNWIGTEDWVLNTEKLAELRKFADDEDLQSEWRAAKKKNKLKVVSLIK ERTGYTVNPDAMFDIQIKRIHEYKRQLLNILGIVYRYKKMKEMSASERKKAFVPRVCIFGGKAFATYVQAKRIVKFITDVGSTINHDPEIGDLLKVIFVP DYNVSVAELLIPASELSQHISTAGMEASGTSNMKFSMNGCILIGSLDGANVEIREEVGEENFFLFGAKADEIVSLRKERAEGKFVPDPSFEEVKQFVRSG VFGSNNYDELIGSLEGNEGFGRADYFLVGKDFPSYVECQEKVDKAYRDQKRWTRMSILNTAGSSKFSSDRTIHEYAKDIWNIK | |||
| Full Sequence |
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| Protein Sequence Length: 934 Download |
| MDTMRISAGA EKIPVKCNSL SSLVTRRWRT KTFPARIRTW RLLSVKSIST NPKTLVTDSE 60 QVASSIKHHS EFTPLFSPEK FELPKAFFAT AQSVRDALIV NWNATYEYYN RVNPKQAYYL 120 SMEFLQGRAL SNAVGNLGLT GAYGDALKSL GFELESVAGQ EPDPALGNGG LGRLASCFLD 180 SLATLNYPAW GYGLRYKYGL FKQIITKDGQ EEAAEDWLEL SNPWEIVRND VSYPIKFYGK 240 VVFGSDGKKR WIGGEDVVAV AYDVPIPGYK TKTTINLRLW STKAPSSDFD LSSYNSGKHT 300 EAAEALFNAE KICYVLYPGD ESDEGKALRL KQQYTLCSAS LQDIVARFET RSGGSVNWEE 360 FPEKVAVQMN DTHPTLCIPE LMRILMDLKG LSWEDAWRIT QRTVAYTNHT VLPEALEKWS 420 LELMEKLLPR HVEIIEKIDE ELVSTIVSEY GTENPDLLKE KLKAMRILEN VELPSAFADV 480 IVKPESKPAI AEKTVDQTKD SKEDAQTVVK KEVEEEETAG VVAEVIPEPP KMVRMANLAV 540 VGGHAVNGVA EIHSEIVKTD VFNDFVKLWP DKFQNKTNGV TPRRWIRFCN PYLSDIITNW 600 IGTEDWVLNT EKLAELRKFA DDEDLQSEWR AAKKKNKLKV VSLIKERTGY TVNPDAMFDI 660 QIKRIHEYKR QLLNILGIVY RYKKMKEMSA SERKKAFVPR VCIFGGKAFA TYVQAKRIVK 720 FITDVGSTIN HDPEIGDLLK VIFVPDYNVS VAELLIPASE LSQHISTAGM EASGTSNMKF 780 SMNGCILIGS LDGANVEIRE EVGEENFFLF GAKADEIVSL RKERAEGKFV PDPSFEEVKQ 840 FVRSGVFGSN NYDELIGSLE GNEGFGRADY FLVGKDFPSY VECQEKVDKA YRDQKRWTRM 900 SILNTAGSSK FSSDRTIHEY AKDIWNIKQV ELP* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00343 | Phosphorylase | 6.0e-151 | 146 | 469 | 324 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 528 | 927 | 405 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 63 | 470 | 412 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| pfam00343 | Phosphorylase | 0 | 531 | 928 | 403 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| TIGR02093 | P_ylase | 0 | 531 | 927 | 402 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACJ11757.1 | 0 | 49 | 933 | 30 | 935 | alpha-1,4 glucan phosphorylase [Gossypium hirsutum] |
| EMBL | CBI22291.1 | 0 | 20 | 933 | 42 | 982 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_189578.1 | 0 | 1 | 933 | 1 | 962 | glucan phosphorylase, putative [Arabidopsis thaliana] |
| Swiss-Prot | P53536 | 0 | 50 | 932 | 85 | 1002 | PHSL_VICFA RecName: Full=Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic; AltName: Full=Starch phosphorylase L; Flags: Precursor |
| RefSeq | XP_002279075.1 | 0 | 20 | 933 | 42 | 958 | PREDICTED: similar to Alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2zb2_B | 0 | 46 | 932 | 14 | 835 | A Chain A, Human Liver Glycogen Phosphorylase A Complexed With Glcose And 5- Chloro-N-[4-(1,2-Dihydroxyethyl)phenyl]-1h-Indole-2-Carboxamide |
| PDB | 2zb2_A | 0 | 46 | 932 | 14 | 835 | A Chain A, Human Liver Glycogen Phosphorylase A Complexed With Glcose And 5- Chloro-N-[4-(1,2-Dihydroxyethyl)phenyl]-1h-Indole-2-Carboxamide |
| PDB | 2qll_A | 0 | 46 | 932 | 12 | 833 | A Chain A, Human Liver Glycogen Phosphorylase- Gl Complex |
| PDB | 1l7x_B | 0 | 46 | 932 | 12 | 833 | A Chain A, Human Liver Glycogen Phosphorylase- Gl Complex |
| PDB | 1l7x_A | 0 | 46 | 932 | 12 | 833 | A Chain A, Human Liver Glycogen Phosphorylase- Gl Complex |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1826 | EC-2.4.1.1 | phosphorylase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO778303 | 864 | 86 | 934 | 0 |
| HO797178 | 384 | 551 | 934 | 0 |
| HO613954 | 512 | 423 | 934 | 0 |
| HO418036 | 585 | 272 | 853 | 0 |
| HO778303 | 120 | 3 | 88 | 0.00007 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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