| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra033571 |
| Family | GH35 |
| Protein Properties | Length: 957 Molecular Weight: 109676 Isoelectric Point: 8.9704 |
| Chromosome | Chromosome/Scaffold: 06 Start: 26171217 End: 26175970 |
| Description | beta-galactosidase 14 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
 | |||
| Family | Start | End | Evalue |
| GH35 | 119 | 423 | 0 |
| LIINGKRELLYSGSVHYPRSTPDMWPSIIEKARVGRLNTIQTYVFWNVHEPEQGKWDFKGRFDLVKFIKLIHEKGLYVTIRLGPFIQAEWNHGGLPVWLR EVPDVYFRTDNEPFKEHTERYVRKILGILKEEKLFASQGGPIILGQIENEYNAVRLAYKENGERYIKWAANLVESMNLGIPWVMCKQDNAPGNIINACNG RHCGDTFPGPKRPDMPSLWTENWTTQFRVFGDPTTNRRSEDIAFAVSRFFSKNGSHVNYYMYHGGTNFGRTSAHFVTTRYYDHAPLDEYGMEKDPKYGHL KHVHR | |||
| Full Sequence |
|---|
| Protein Sequence Length: 957 Download |
| MNSLHVNKVI FAGDFEDLFG AVLRPQAWPS FLFQRDEFIR SVAGIEEWRV RVVKNETNRG 60 ASFIADSVNR HRLVQSYVAA GPPSWLVEFF ESYAKEEKDD DENNKKKNKK GVTYDGTSLI 120 INGKRELLYS GSVHYPRSTP DMWPSIIEKA RVGRLNTIQT YVFWNVHEPE QGKWDFKGRF 180 DLVKFIKLIH EKGLYVTIRL GPFIQAEWNH GGLPVWLREV PDVYFRTDNE PFKEHTERYV 240 RKILGILKEE KLFASQGGPI ILGQIENEYN AVRLAYKENG ERYIKWAANL VESMNLGIPW 300 VMCKQDNAPG NIINACNGRH CGDTFPGPKR PDMPSLWTEN WTTQFRVFGD PTTNRRSEDI 360 AFAVSRFFSK NGSHVNYYMY HGGTNFGRTS AHFVTTRYYD HAPLDEYGME KDPKYGHLKH 420 VHRALELCKK ALLWGHPRFQ KLGPDTEVRY YEQPGTNVCA AFLANNNTRE PNTVKFKGQK 480 FVLPSRSISI LPDCKTVVYN TAQIVAQHSW RNFVKSEKSS KGLKFDMYSE NVPSKLEGDT 540 LIPGELYYLT KDKTDYAWYT TSIKIKEDDL PDKKGVKTIL RVASLGHAAI VYVNGEYVGN 600 KHGSHEMKSF VFKKPVKLKP GDNHISILGV LTGFPDSGSY MEKRFAGPRG ASIIGLKSGE 660 RDLTENNHWG HLAGLEGEKK EVYTEQGSKK VKWVKNGERK PLTWYKTYFE TPEGENTVAI 720 RMNGMGKGLI WVNGIGIGRY WMSFLSPTGQ PTQSEYHIPR TFMKKKEKNM LVIFEEEPGV 780 KLEAIDFVLV NRDTICSFVE ENYPASVKYW KRQGPNIVPR NKDMRLKSLI KCPPGKQIVS 840 VEFASFGDPT GTCGNFTMGK CSASKSKEVV EKKCLGKSRC SIVVERETFE DKKCQDIVKT 900 LAVQVKCEKK QGNEQKHKRE DAEEEDDDEE EEEEEEKERE GNNQDLKDRE KRKQDH* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
 | ||||||||
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam02449 | Glyco_hydro_42 | 2.0e-7 | 134 | 269 | 154 | + Beta-galactosidase. This group of beta-galactosidase enzymes belong to the glycosyl hydrolase 42 family. The enzyme catalyzes the hydrolysis of terminal, non-reducing terminal beta-D-galactosidase residues. | ||
| pfam02140 | Gal_Lectin | 4.0e-11 | 830 | 907 | 81 | + Galactose binding lectin domain. | ||
| COG1874 | LacA | 3.0e-19 | 112 | 300 | 197 | + Beta-galactosidase [Carbohydrate transport and metabolism] | ||
| pfam01301 | Glyco_hydro_35 | 9.0e-142 | 118 | 425 | 322 | + Glycosyl hydrolases family 35. | ||
| PLN03059 | PLN03059 | 0 | 112 | 907 | 824 | + beta-galactosidase; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0004565 | beta-galactosidase activity |
| GO:0005529 | Interacting selectively and non-covalently with any carbohydrate, which includes monosaccharides, oligosaccharides and polysaccharides as well as substances derived from monosaccharides by reduction of the carbonyl group (alditols), by oxidation of one or more hydroxy groups to afford the corresponding aldehydes, ketones, or carboxylic acids, or by replacement of one or more hydroxy group(s) by a hydrogen atom. Cyclitols are generally not regarded as carbohydrates." [CHEBI:16646, GOC:mah] |
| GO:0005975 | carbohydrate metabolic process |
| GO:0009341 | beta-galactosidase complex |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAB37515.1 | 0 | 173 | 955 | 80 | 864 | galactosidase like protein [Arabidopsis thaliana] |
| EMBL | CAB64750.1 | 0 | 94 | 955 | 27 | 886 | putative beta-galactosidase [Arabidopsis thaliana] |
| RefSeq | NP_001154292.1 | 0 | 94 | 914 | 19 | 838 | glycosyl hydrolase family 35 protein [Arabidopsis thaliana] |
| RefSeq | NP_195571.2 | 0 | 142 | 955 | 1 | 816 | glycosyl hydrolase family 35 protein [Arabidopsis thaliana] |
| Swiss-Prot | Q9SCU8 | 0 | 94 | 955 | 27 | 886 | BGL14_ARATH RecName: Full=Beta-galactosidase 14; Short=Lactase 14; Flags: Precursor |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3d3a_A | 2e-40 | 118 | 421 | 14 | 325 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
| PDB | 3d3a_A | 0.001 | 683 | 773 | 490 | 571 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
| PDB | 3thd_D | 8e-33 | 104 | 426 | 3 | 335 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
| PDB | 3thd_D | 0.003 | 685 | 826 | 508 | 651 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
| PDB | 3thd_C | 8e-33 | 104 | 426 | 3 | 335 | A Chain A, Crystal Structure Of A Beta-Galactosidase From Bacteroides Thetaiotaomicron |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| lactose degradation III | BETAGALACTOSID-RXN | EC-3.2.1.23 | β-galactosidase |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
 | ||||
| Hit | Length | Start | End | EValue |
| HO778160 | 695 | 112 | 779 | 0 |
| HO780602 | 593 | 162 | 746 | 0 |
| GR453801 | 261 | 204 | 464 | 0 |
| CO128575 | 295 | 141 | 435 | 0 |
| HO795552 | 530 | 202 | 723 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
