| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra033739 |
| Family | AA7 |
| Protein Properties | Length: 609 Molecular Weight: 68370.5 Isoelectric Point: 8.1205 |
| Chromosome | Chromosome/Scaffold: 06 Start: 25324532 End: 25333472 |
| Description | FAD-binding Berberine family protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 73 | 512 | 0 |
| LRYVTESMPKPGFIFKPVHESHVQASVICSNQLGLHHRVRSGGHDYEGLSFVSLMETPFILIDLSKLRQINVDIEDNSAWVQSGATIGELYYRIAEKSKL HGFPAGLCSTVGIGGHITGGGDGTLLRKYGLAADNVLDAKIVNADGKLLNRAAMGEDLFWAIRGGGGGSFGVILAWKLKLVPVPETLTMFTVTKTLEQDP ETKILSKWQRIADKLVDDLFLRVMFRVAGDKTLTLEYKGQFLGEKGTLMEVMKKYFPELGLTQEDCIEVTWIESVLANAGFPIDSPLEVLLNPKLSPYVK VYFKGKSDFATEPIPPFGLKGMFKKLVEENTSSMFMIPYGGMMAKIPESETPFAHRKGTILKIHYATNSTSEDGISLNKRTKWIREMYSYMTPYVSSNPR LAYVNYRDLDLGTNTKDSKANFIKAQTWGAQYFKNNFNRL | |||
| Full Sequence |
|---|
| Protein Sequence Length: 609 Download |
| MGKSKALYTF SFLAVYFSFC TITPTSSTSL QDDFINCLHE NTNVDFPLLE TFFSPERNVS 60 IYKEVLQSTA HNLRYVTESM PKPGFIFKPV HESHVQASVI CSNQLGLHHR VRSGGHDYEG 120 LSFVSLMETP FILIDLSKLR QINVDIEDNS AWVQSGATIG ELYYRIAEKS KLHGFPAGLC 180 STVGIGGHIT GGGDGTLLRK YGLAADNVLD AKIVNADGKL LNRAAMGEDL FWAIRGGGGG 240 SFGVILAWKL KLVPVPETLT MFTVTKTLEQ DPETKILSKW QRIADKLVDD LFLRVMFRVA 300 GDKTLTLEYK GQFLGEKGTL MEVMKKYFPE LGLTQEDCIE VTWIESVLAN AGFPIDSPLE 360 VLLNPKLSPY VKVYFKGKSD FATEPIPPFG LKGMFKKLVE ENTSSMFMIP YGGMMAKIPE 420 SETPFAHRKG TILKIHYATN STSEDGISLN KRTKWIREMY SYMTPYVSSN PRLAYVNYRD 480 LDLGTNTKDS KANFIKAQTW GAQYFKNNFN RLEVEIPTPR HENNIQNINY FTKVLSASDT 540 NKKGSFVLNK RHAIECLPLL VQWDESATIP RPDKVSPWEI EPLTHSSNIL KSVFLKNKRQ 600 SEIDDFGK* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR00387 | glcD | 0.0005 | 120 | 258 | 148 | + glycolate oxidase, subunit GlcD. This protein, the glycolate oxidase GlcD subunit, is similar in sequence to that of several D-lactate dehydrogenases, including that of E. coli. The glycolate oxidase has been found to have some D-lactate dehydrogenase activity [Energy metabolism, Other]. | ||
| pfam08031 | BBE | 4.0e-5 | 474 | 525 | 59 | + Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine. | ||
| COG0277 | GlcD | 4.0e-17 | 65 | 311 | 262 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 2.0e-21 | 83 | 222 | 141 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0016491 | oxidoreductase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAL24314.1 | 0 | 1 | 528 | 1 | 540 | berberine bridge enzyme-like protein [Arabidopsis thaliana] |
| RefSeq | NP_199251.1 | 0 | 1 | 530 | 1 | 541 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| RefSeq | NP_199252.1 | 0 | 1 | 528 | 1 | 540 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| RefSeq | NP_199253.1 | 0 | 1 | 512 | 1 | 514 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| RefSeq | XP_002523164.1 | 0 | 32 | 525 | 31 | 528 | Reticuline oxidase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 4dns_B | 0 | 24 | 531 | 3 | 487 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 4dns_A | 0 | 24 | 531 | 3 | 487 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 3vte_A | 0 | 31 | 524 | 4 | 502 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 3tsj_B | 0 | 29 | 514 | 6 | 476 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 3tsj_A | 0 | 29 | 514 | 6 | 476 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cannabinoid biosynthesis | RXN-7854 | EC-1.21.3 | tetrahydrocannabinolic acid synthase |
