| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra033740 |
| Family | AA7 |
| Protein Properties | Length: 548 Molecular Weight: 62297.9 Isoelectric Point: 9.6054 |
| Chromosome | Chromosome/Scaffold: 06 Start: 25321018 End: 25322661 |
| Description | FAD-binding Berberine family protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 95 | 542 | 0 |
| SSEKPILIATPRTESEIQRSLLCSRKLGVQVRTKSGGHDYEGLSYLSSQSPFIVLDLVNLRSIDINLREETAWVGAGATIGELYYKIAKASKIHGFPAGT CPSVGVGGHFSGGGFGAMMRKHGLAADNVVDARFVDANGRIYNGRREMGEDLFWAIRGGGAASFGVVTSWKVKLVRVPEKVTCFTRYLPFTQNMTKIVHR WQSIAAALDNDLFIRVIIYNSGGSVQATFQANYLGGIDKLIPLMNEKFPELGLRFQDCSEMSWIDSIMYFNWKRGQPLETLLDREQRYNDLYFKAKSDFV KNPIPETGLEGIWKRFHEVESPMMIMEPLGGRMYEIGETETPFPHRIGNLYNIQYMVKWRVKDIEEMEKYVRWMRLLYGYMRVYVSGSPRGAYLNYRDLD LGMNKAINCSFEDASLWGMRYFGSNFKRLAMVKGKIDPTNFFRNEQSV | |||
| Full Sequence |
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| Protein Sequence Length: 548 Download |
| MLFLCNSKLK KTKALKMRTV EAFALSLLLL FLVKWANSDS KVSPIRDQFL SCMSTHSVSS 60 FMNSKSLIHE PDSRLYTDLS QTVSQNYRFS SLNFSSEKPI LIATPRTESE IQRSLLCSRK 120 LGVQVRTKSG GHDYEGLSYL SSQSPFIVLD LVNLRSIDIN LREETAWVGA GATIGELYYK 180 IAKASKIHGF PAGTCPSVGV GGHFSGGGFG AMMRKHGLAA DNVVDARFVD ANGRIYNGRR 240 EMGEDLFWAI RGGGAASFGV VTSWKVKLVR VPEKVTCFTR YLPFTQNMTK IVHRWQSIAA 300 ALDNDLFIRV IIYNSGGSVQ ATFQANYLGG IDKLIPLMNE KFPELGLRFQ DCSEMSWIDS 360 IMYFNWKRGQ PLETLLDREQ RYNDLYFKAK SDFVKNPIPE TGLEGIWKRF HEVESPMMIM 420 EPLGGRMYEI GETETPFPHR IGNLYNIQYM VKWRVKDIEE MEKYVRWMRL LYGYMRVYVS 480 GSPRGAYLNY RDLDLGMNKA INCSFEDASL WGMRYFGSNF KRLAMVKGKI DPTNFFRNEQ 540 SVPPLIV* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG0277 | GlcD | 4.0e-16 | 99 | 545 | 478 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam08031 | BBE | 2.0e-17 | 486 | 543 | 58 | + Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine. | ||
| pfam01565 | FAD_binding_4 | 5.0e-21 | 99 | 237 | 140 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0016491 | oxidoreductase activity |
| GO:0050660 | flavin adenine dinucleotide binding |
| GO:0055114 | oxidation-reduction process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAK56258.1 | 0 | 43 | 545 | 23 | 529 | AF367269_1 AT4g20860/T13K14_20 [Arabidopsis thaliana] |
| RefSeq | NP_193818.1 | 0 | 43 | 545 | 23 | 529 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| RefSeq | NP_199249.1 | 0 | 17 | 547 | 1 | 532 | FAD-binding domain-containing protein [Arabidopsis thaliana] |
| RefSeq | XP_002267029.1 | 0 | 16 | 545 | 242 | 770 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002330615.1 | 0 | 35 | 545 | 24 | 532 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3vte_A | 0 | 46 | 545 | 4 | 513 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 4dns_B | 0 | 45 | 546 | 9 | 497 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 4dns_A | 0 | 45 | 546 | 9 | 497 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 3tsj_B | 0 | 46 | 546 | 8 | 497 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 3tsj_A | 0 | 46 | 546 | 8 | 497 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cannabinoid biosynthesis | RXN-7854 | EC-1.21.3 | tetrahydrocannabinolic acid synthase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| GT085426 | 304 | 211 | 514 | 0 |
| FG559455 | 249 | 233 | 481 | 0 |
| FG563587 | 235 | 173 | 407 | 0 |
| HO777438 | 515 | 46 | 545 | 0 |
| ES268640 | 209 | 340 | 548 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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