| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra034558 |
| Family | GT8 |
| Protein Properties | Length: 593 Molecular Weight: 69653.3 Isoelectric Point: 9.3743 |
| Chromosome | Chromosome/Scaffold: 08 Start: 12832721 End: 12836074 |
| Description | plant glycogenin-like starch initiation protein 3 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT8 | 293 | 509 | 3.80004e-41 |
| RRPKREAYATVLHSSESYVCGAITLAQSLLKTNTKRDLILLHDDSISITKLRALAAAGWKLRRILRIRNPLAEKDSYNEYNYSKFRLWQLTDYDKVVFID ADIIVLLNLDLLFHFPQMSATGNDVWIFNSGVMVIEPSNCTFSTIMSLRNDIVSYNGGDQGYLNEIFVWWHRLPRRVNFLKNFWSNTTNERNIKNSLFAA EPPQLYAVHYLGWKPWL | |||
| Full Sequence |
|---|
| Protein Sequence Length: 593 Download |
| MMMVMKIAPS KSTLIRFNLI LFALSFLLYT AFSLSPSSSV YFRSAASFVG CSFRHCTPKV 60 TRGVKMQELI VENQINKIGS QIASNQTKLE APSFMEEILT RGLGKTKIGM VNMEEADLTQ 120 WKRYGETNHI HFDRVSKLFT WHDLFPEWID EEEDHEVPTC PEIPMPDLER LEKFDLIVVK 180 LPCKYPEEGW RREVLRLQVN LVAANLVAKK GKTDWTWKSK VLFWSKCQPM IEIFRCDDME 240 KREGNWWLYR PEVVRLQQKV SLPIGSCNLA LPLWAPQGID KVYDLTKIKA ETRRPKREAY 300 ATVLHSSESY VCGAITLAQS LLKTNTKRDL ILLHDDSISI TKLRALAAAG WKLRRILRIR 360 NPLAEKDSYN EYNYSKFRLW QLTDYDKVVF IDADIIVLLN LDLLFHFPQM SATGNDVWIF 420 NSGVMVIEPS NCTFSTIMSL RNDIVSYNGG DQGYLNEIFV WWHRLPRRVN FLKNFWSNTT 480 NERNIKNSLF AAEPPQLYAV HYLGWKPWLC YRDYDCNFDV EQHVYANDAA HARWWKVHDA 540 MDEDLQRFCR LTKKRRTELN WERRRSRRRG STDFHWRINV TDPRRRRSYL IG* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN00176 | PLN00176 | 1.0e-10 | 275 | 408 | 143 | + galactinol synthase | ||
| cd04194 | GT8_A4GalT_like | 3.0e-12 | 306 | 508 | 247 | + A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS. | ||
| cd06914 | GT8_GNT1 | 2.0e-14 | 300 | 438 | 146 | + GNT1 is a fungal enzyme that belongs to the GT 8 family. N-acetylglucosaminyltransferase is a fungal enzyme that catalyzes the addition of N-acetyl-D-glucosamine to mannotetraose side chains by an alpha 1-2 linkage during the synthesis of mannan. The N-acetyl-D-glucosamine moiety in mannan plays a role in the attachment of mannan to asparagine residues in proteins. The mannotetraose and its N-acetyl-D-glucosamine derivative side chains of mannan are the principle immunochemical determinants on the cell surface. N-acetylglucosaminyltransferase is a member of glycosyltransferase family 8, which are, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed, retaining glycosyltransferases. | ||
| pfam01501 | Glyco_transf_8 | 2.0e-41 | 301 | 511 | 253 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd02537 | GT8_Glycogenin | 9.0e-69 | 298 | 541 | 254 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAB38791.1 | 0 | 278 | 592 | 18 | 333 | putative protein [Arabidopsis thaliana] |
| RefSeq | NP_001154284.1 | 0 | 8 | 586 | 11 | 590 | transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | NP_195059.3 | 0 | 8 | 592 | 11 | 596 | transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002282762.1 | 0 | 3 | 585 | 11 | 588 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002510223.1 | 0 | 1 | 586 | 7 | 586 | glycogenin, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1zcv_A | 2e-19 | 298 | 508 | 24 | 240 | A Chain A, Apo Form Of A Mutant Of Glycogenin In Which Asp159 Is Replaced By Asn |
| PDB | 3u2x_B | 3e-19 | 298 | 508 | 5 | 221 | A Chain A, Apo Form Of A Mutant Of Glycogenin In Which Asp159 Is Replaced By Asn |
| PDB | 3u2x_A | 3e-19 | 298 | 508 | 5 | 221 | A Chain A, Apo Form Of A Mutant Of Glycogenin In Which Asp159 Is Replaced By Asn |
| PDB | 3u2v_B | 3e-19 | 298 | 508 | 5 | 221 | A Chain A, Apo Form Of A Mutant Of Glycogenin In Which Asp159 Is Replaced By Asn |
| PDB | 3u2v_A | 3e-19 | 298 | 508 | 5 | 221 | A Chain A, Apo Form Of A Mutant Of Glycogenin In Which Asp159 Is Replaced By Asn |
