PlantCAZyme

Basic Information
Species	Brassica rapa
Cazyme ID	Bra034569
Family	CE8
Protein Properties	Length: 363 Molecular Weight: 38751.1 Isoelectric Point: 7.529
Chromosome	Chromosome/Scaffold: 08 Start: 12790101 End: 12791189
Description	Plant invertase/pectin methylesterase inhibitor superfamily
View CDS

External Links
NCBI Taxonomy
CAZyDB

Signature Domain Download full data set without filtering

Family	Start	End	Evalue
CE8	273	358	1.5e-24
DVTVCKDGGKGCGYKTVQEAVDAAPGSNGDVKFVIGIREGVYEETVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTYNSATV

Full Sequence
Protein Sequence Length: 363 Download
MDSPKLPHSL STSSSTPLAS SAVKPHRKNL PPRAYILIII AVSAILLLLL TLILYANVSK 60 SSHNRHNTPQ QPPPPPTAQI RLACNATRFP DHCFSSLSKP GLVPQDPKPV QIIHSAISVS 120 YESLKSGQSQ IKSILDSSAG DKNRTNIATI CLEILSYSQH RTESSDVAVT SGGIKDARAW 180 MSAALAYQYD CWSGLKTVND TKQVVDTITF LDDLVKLTGT ALSMMVSFDN FGDDVVSWIH 240 PATERDGFWE KTGPSSGPVK EAGLSFPSGL KEDVTVCKDG GKGCGYKTVQ EAVDAAPGSN 300 GDVKFVIGIR EGVYEETVRV PFEKKNVVFI GDGMGKTVIT GSLNVGQPGM TTYNSATVGE 360 LI*

Full Sequence

Protein Sequence Length: 363 Download

MDSPKLPHSL STSSSTPLAS SAVKPHRKNL PPRAYILIII AVSAILLLLL TLILYANVSK    60
SSHNRHNTPQ QPPPPPTAQI RLACNATRFP DHCFSSLSKP GLVPQDPKPV QIIHSAISVS    120
YESLKSGQSQ IKSILDSSAG DKNRTNIATI CLEILSYSQH RTESSDVAVT SGGIKDARAW    180
MSAALAYQYD CWSGLKTVND TKQVVDTITF LDDLVKLTGT ALSMMVSFDN FGDDVVSWIH    240
PATERDGFWE KTGPSSGPVK EAGLSFPSGL KEDVTVCKDG GKGCGYKTVQ EAVDAAPGSN    300
GDVKFVIGIR EGVYEETVRV PFEKKNVVFI GDGMGKTVIT GSLNVGQPGM TTYNSATVGE    360
LI* 

Functional Domains Download unfiltered results here

Cdd ID	Domain	E-Value	Start	End	Length	Domain Description
PLN02990	PLN02990	5.0e-24	61	358	316	+ Probable pectinesterase/pectinesterase inhibitor
smart00856	PMEI	1.0e-24	80	224	145	+ Plant invertase/pectin methylesterase inhibitor. This domain inhibits pectin methylesterases (PMEs) and invertases through formation of a non-covalent 1:1 complex. It has been implicated in the regulation of fruit development, carbohydrate metabolism and cell wall extension. It may also be involved in inhibiting microbial pathogen PMEs. It has been observed that it is often expressed as a large inactive preprotein. It is also found at the N-termini of PMEs predicted from DNA sequences, suggesting that both PMEs and their inhibitors are expressed as a single polyprotein and subsequently processed. It has two disulphide bridges and is mainly alpha-helical.
pfam01095	Pectinesterase	5.0e-31	273	359	87	+ Pectinesterase.
PLN02301	PLN02301	1.0e-32	124	358	247	+ pectinesterase/pectinesterase inhibitor
PLN02708	PLN02708	9.0e-168	58	361	313	+ Probable pectinesterase/pectinesterase inhibitor

Gene Ontology
GO Term	Description
GO:0004857	enzyme inhibitor activity
GO:0005618	cell wall
GO:0030599	pectinesterase activity
GO:0042545	cell wall modification

Annotations - NR Download unfiltered results here
Source	Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
DDBJ	BAB09534.1	0	68	359	59	350	pectin methylesterase-like protein [Arabidopsis thaliana]
RefSeq	NP_196538.1	0	68	359	33	324	pectinesterase family protein [Arabidopsis thaliana]
RefSeq	NP_568991.2	0	1	361	1	376	pectinesterase family protein [Arabidopsis thaliana]
RefSeq	XP_002275218.1	0	65	359	46	346	PREDICTED: hypothetical protein [Vitis vinifera]
RefSeq	XP_002281640.1	0	65	359	28	328	PREDICTED: hypothetical protein [Vitis vinifera]

Annotations - PDB Download unfiltered results here
Source	Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
PDB	1gq8_A	0.000000000000003	273	361	8	92	A Chain A, Pectin Methylesterase From Carrot
PDB	1xg2_A	0.0000000006	286	361	15	88	B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein

EST Download unfiltered results here

Hit	Length	Start	End	EValue
DK466132	276	23	298	0
DK479670	260	23	282	0
EX021538	244	80	320	0
EX021807	214	121	331	0
DK478318	203	23	225	0

Orthologous Group
Species	ID
Linum usitatissimum	Lus10013720
Picea abies	MA_10629g0010	MA_10430460g0010.217.314	MA_10430460g0010.217.314

CAZyme Information