| Basic Information | |
|---|---|
| Species | Brassica rapa |
| Cazyme ID | Bra034569 |
| Family | CE8 |
| Protein Properties | Length: 363 Molecular Weight: 38751.1 Isoelectric Point: 7.529 |
| Chromosome | Chromosome/Scaffold: 08 Start: 12790101 End: 12791189 |
| Description | Plant invertase/pectin methylesterase inhibitor superfamily |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CE8 | 273 | 358 | 1.5e-24 |
| DVTVCKDGGKGCGYKTVQEAVDAAPGSNGDVKFVIGIREGVYEETVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTYNSATV | |||
| Full Sequence |
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| Protein Sequence Length: 363 Download |
| MDSPKLPHSL STSSSTPLAS SAVKPHRKNL PPRAYILIII AVSAILLLLL TLILYANVSK 60 SSHNRHNTPQ QPPPPPTAQI RLACNATRFP DHCFSSLSKP GLVPQDPKPV QIIHSAISVS 120 YESLKSGQSQ IKSILDSSAG DKNRTNIATI CLEILSYSQH RTESSDVAVT SGGIKDARAW 180 MSAALAYQYD CWSGLKTVND TKQVVDTITF LDDLVKLTGT ALSMMVSFDN FGDDVVSWIH 240 PATERDGFWE KTGPSSGPVK EAGLSFPSGL KEDVTVCKDG GKGCGYKTVQ EAVDAAPGSN 300 GDVKFVIGIR EGVYEETVRV PFEKKNVVFI GDGMGKTVIT GSLNVGQPGM TTYNSATVGE 360 LI* 420 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02990 | PLN02990 | 5.0e-24 | 61 | 358 | 316 | + Probable pectinesterase/pectinesterase inhibitor | ||
| smart00856 | PMEI | 1.0e-24 | 80 | 224 | 145 | + Plant invertase/pectin methylesterase inhibitor. This domain inhibits pectin methylesterases (PMEs) and invertases through formation of a non-covalent 1:1 complex. It has been implicated in the regulation of fruit development, carbohydrate metabolism and cell wall extension. It may also be involved in inhibiting microbial pathogen PMEs. It has been observed that it is often expressed as a large inactive preprotein. It is also found at the N-termini of PMEs predicted from DNA sequences, suggesting that both PMEs and their inhibitors are expressed as a single polyprotein and subsequently processed. It has two disulphide bridges and is mainly alpha-helical. | ||
| pfam01095 | Pectinesterase | 5.0e-31 | 273 | 359 | 87 | + Pectinesterase. | ||
| PLN02301 | PLN02301 | 1.0e-32 | 124 | 358 | 247 | + pectinesterase/pectinesterase inhibitor | ||
| PLN02708 | PLN02708 | 9.0e-168 | 58 | 361 | 313 | + Probable pectinesterase/pectinesterase inhibitor | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004857 | enzyme inhibitor activity |
| GO:0005618 | cell wall |
| GO:0030599 | pectinesterase activity |
| GO:0042545 | cell wall modification |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAB09534.1 | 0 | 68 | 359 | 59 | 350 | pectin methylesterase-like protein [Arabidopsis thaliana] |
| RefSeq | NP_196538.1 | 0 | 68 | 359 | 33 | 324 | pectinesterase family protein [Arabidopsis thaliana] |
| RefSeq | NP_568991.2 | 0 | 1 | 361 | 1 | 376 | pectinesterase family protein [Arabidopsis thaliana] |
| RefSeq | XP_002275218.1 | 0 | 65 | 359 | 46 | 346 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002281640.1 | 0 | 65 | 359 | 28 | 328 | PREDICTED: hypothetical protein [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1gq8_A | 0.000000000000003 | 273 | 361 | 8 | 92 | A Chain A, Pectin Methylesterase From Carrot |
| PDB | 1xg2_A | 0.0000000006 | 286 | 361 | 15 | 88 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
| Transmembrane Domains | |||||
|---|---|---|---|---|---|
| Start | End | ||||
| 34 | 56 | ||||
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DK466132 | 276 | 23 | 298 | 0 |
| DK479670 | 260 | 23 | 282 | 0 |
| EX021538 | 244 | 80 | 320 | 0 |
| EX021807 | 214 | 121 | 331 | 0 |
| DK478318 | 203 | 23 | 225 | 0 |
| Orthologous Group | |||||
|---|---|---|---|---|---|
| Species | ID | ||||
| Linum usitatissimum | Lus10013720 | ||||
| Picea abies | MA_10629g0010 | MA_10430460g0010.217.314 | MA_10430460g0010.217.314 | ||
| Sequence Alignments (This image is cropped. Click for full image.) |
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| Phylogeny |
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